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Literature summary extracted from

  • Breitung, J.; Börner, G.; Scholz, S.; Linder, D.; Stetter, K.O.; Thauer, R.K.
    Salt dependence, kinetic properties and catalytic mechanism of N-formylmethanofuran:tetrahydromethanopterin formyltransferase from the extreme thermophile Methanopyrus kandleri (1992), Eur. J. Biochem., 210, 971-981.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.3.1.101 presence of salts, 1.5 M, required for optimal stabilization, order of efficiency in protecting the enzyme from heat inactivation at 90°C: K2HPO4, (NH4)2SO4, KCl, NH4Cl, NaCl, Na2SO4, Na2HPO4 Methanopyrus kandleri

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.101 0.05
-
 formylmethanofuran
-
 Methanopyrus kandleri
2.3.1.101 0.1
-
 5,6,7,8-tetrahydromethanopterin
-
 Methanopyrus kandleri
2.3.1.101 20
-
 N-Furfurylformamide
-
 Methanopyrus kandleri

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.1.101 (NH4)2SO4
-
 Methanopyrus kandleri
2.3.1.101 K2HPO4 no activity in absence, assay mixture contains 2 M Methanopyrus kandleri
2.3.1.101 salts absolutely dependent on the presence of phosphate or sulfate salts for activity, efficiency of activation in decreasing order: K2HPO4, (NH4)2SO4, K2SO4, Na2SO4, Na2HPO4, no activation: NaCl, KCl, NH4Cl Methanopyrus kandleri

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.101 35000
-
 1 * 35000, SDS-PAGE Methanopyrus kandleri

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
2.3.1.101 additional information enzyme completely soluble in 80% ammonium sulfate Methanopyrus kandleri

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.101 Methanopyrus kandleri
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.101
-
 Methanopyrus kandleri

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.1.101 formylmethanofuran + 5,6,7,8-tetrahydromethanopterin = methanofuran + 5-formyl-5,6,7,8-tetrahydromethanopterin ternary complex type mechanism Methanopyrus kandleri
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.101 additional information
-
-
 Methanopyrus kandleri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.101 formylmethanofuran + 5,6,7,8-tetrahydromethanopterin specific for tetrahydromethanopterin Methanopyrus kandleri methanofuran + N5-formyl-5,6,7,8-tetrahydromethanopterin
-
 ?
2.3.1.101 N-furfurylformamide + 5,6,7,8-tetrahydromethanopterin pseudo-substrate Methanopyrus kandleri 2-(aminomethyl)furane + N5-formyl-5,6,7,8-tetrahydromethanopterin
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.101 monomer 1 * 35000, SDS-PAGE Methanopyrus kandleri

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.101 90
-
-
 Methanopyrus kandleri

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.1.101 90
-
 stable up to, in presence of salts Methanopyrus kandleri

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.101 6.5
-
-
 Methanopyrus kandleri