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Literature summary extracted from

  • Schwörer, B.; Breitung, J.; Klein, A.R.; Stetter, K.O.; Thauer, R.K.
    Formylmethanofuran:tetrahydromethanopterin formyltransferase and N5,N10-methylenetetrahydromethanopterin dehydrogenase from the sulfate-reducing Archaeoglobus fulgidus: similarities with the enzymes from methanogenic Archaea (1993), Arch. Microbiol., 159, 225-232.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.5.98.1 thermostable up to 90°C, only in the presence of salts. Complete stability in presence of 0.4-0.5 M K2HPO4 Archaeoglobus fulgidus
2.3.1.101 salts protect against heat inactivation, order of efficiency: K2HPO4, (NH4)2SO4, KCl, NH4Cl, Na2SO4, Na2HPO4 Archaeoglobus fulgidus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.98.1 (NH4)2SO4 0.15M-0.2M (NH4)2SO4 stimulates by a factor of 1.3. Higher concentrations are inhibitory Archaeoglobus fulgidus
1.5.98.1 K2HPO4 0.15M-0.2M K2HPO4 stimulates by a factor of 1.3. Higher concentrations are inhibitory Archaeoglobus fulgidus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.98.1 0.013
-
 coenzyme F420
-
 Archaeoglobus fulgidus
1.5.98.1 0.017
-
 5,10-methylenetetrahydromethanopterin
-
 Archaeoglobus fulgidus
2.3.1.101 0.017
-
 5,6,7,8-tetrahydromethanopterin
-
 Archaeoglobus fulgidus
2.3.1.101 0.032
-
 formylmethanofuran
-
 Archaeoglobus fulgidus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.5.98.1 (NH4)2SO4 0.15M-0.2M (NH4)2SO4 stimulates by a factor of 1.3. Higher concentrations are inhibitory Archaeoglobus fulgidus
1.5.98.1 K+ 0.15M-0.2M K2HPO4 stimulates by a factor of 1.3. Higher concentrations are inhibitory Archaeoglobus fulgidus
2.3.1.101 (NH4)2SO4 can substitute for K2HPO4 in stimulation Archaeoglobus fulgidus
2.3.1.101 K2HPO4 1.5 M stimulates 3.8fold Archaeoglobus fulgidus
2.3.1.101 K2HPO4 no activity in absence, assay mixture contains 2 M Archaeoglobus fulgidus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.98.1 32000
-
 x * 32000, SDS-PAGE Archaeoglobus fulgidus
1.5.98.1 140000
-
 gel filtration Archaeoglobus fulgidus
2.3.1.101 30000
-
 4 * 30000 SDS-PAGE Archaeoglobus fulgidus
2.3.1.101 125000
-
 PAGE Archaeoglobus fulgidus

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
2.3.1.101 additional information enzyme soluble in 60% ammonium sulfate Archaeoglobus fulgidus

Organism

EC Number Organism UniProt Comment Textmining
1.5.98.1 Archaeoglobus fulgidus
-
-
-
2.3.1.101 Archaeoglobus fulgidus
-
-
-
2.3.1.101 Archaeoglobus fulgidus VC-19 / DSM 4304
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
2.3.1.101 relatively insensitive towards inactivation by molecular oxygen Archaeoglobus fulgidus

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.98.1
-
 Archaeoglobus fulgidus
2.3.1.101
-
 Archaeoglobus fulgidus

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.1.101 formylmethanofuran + 5,6,7,8-tetrahydromethanopterin = methanofuran + 5-formyl-5,6,7,8-tetrahydromethanopterin ternary complex type mechanism Archaeoglobus fulgidus
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.98.1 1560
-
-
 Archaeoglobus fulgidus
2.3.1.101 1415
-
-
 Archaeoglobus fulgidus

Storage Stability

EC Number Storage Stability Organism
1.5.98.1 -20°C, stable for several weeks Archaeoglobus fulgidus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.98.1 5,10-methylenetetrahydromethanopterin + oxidized coenzyme F420 r Archaeoglobus fulgidus 5,10-methenyltetrahydromethanopterin + reduced coenzyme F420
-
 ?
1.5.98.1 5,10-methylenetetrahydromethanopterin + oxidized coenzyme F420 r Archaeoglobus fulgidus VC-19 / DSM 4304 5,10-methenyltetrahydromethanopterin + reduced coenzyme F420
-
 ?
2.3.1.101 formylmethanofuran + 5,6,7,8-tetrahydromethanopterin
-
 Archaeoglobus fulgidus methanofuran + N5-formyl-5,6,7,8-tetrahydromethanopterin
-
 ?
2.3.1.101 formylmethanofuran + 5,6,7,8-tetrahydromethanopterin
-
 Archaeoglobus fulgidus VC-19 / DSM 4304 methanofuran + N5-formyl-5,6,7,8-tetrahydromethanopterin
-
 ?

Subunits

EC Number Subunits Comment Organism
1.5.98.1 ? x * 32000, SDS-PAGE Archaeoglobus fulgidus
2.3.1.101 tetramer 4 * 30000 SDS-PAGE Archaeoglobus fulgidus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.5.98.1 70
-
-
 Archaeoglobus fulgidus
2.3.1.101 70
-
-
 Archaeoglobus fulgidus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.5.98.1 90
-
 thermostable up to 90°C, only in the presence of salts. Complete stability in presence of 0.4-0.5 M K2HPO4 Archaeoglobus fulgidus
2.3.1.101 80
-
 complete thermostability in presence of 0.5 M K2HPO4 Archaeoglobus fulgidus
2.3.1.101 90
-
 stable up to, in presence of salts Archaeoglobus fulgidus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.98.1 5.5
-
-
 Archaeoglobus fulgidus
2.3.1.101 5
-
-
 Archaeoglobus fulgidus

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.98.1 coenzyme F420 required Archaeoglobus fulgidus