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Literature summary extracted from

  • Donnelly, M.I.; Wolfe, R.S.
    The role of formylmethanofuran:tetrahydromethanopterin formyltransferase in methanogenesis from carbon dioxide (1986), J. Biol. Chem., 261, 16652-16659.
No PubMed abstract available

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.101 41000
-
 4 * 41000, SDS-PAGE before and after treatment with dimethylsuberimidate Methanothermobacter thermautotrophicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.101 formylmethanofuran + 5,6,7,8-tetrahydromethanopterin Methanothermobacter thermautotrophicus involved in the formation of methane from CO2 methanofuran + N5-formyl-5,6,7,8-tetrahydromethanopterin
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.101 Methanothermobacter thermautotrophicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.101
-
 Methanothermobacter thermautotrophicus

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.1.101 formylmethanofuran + 5,6,7,8-tetrahydromethanopterin = methanofuran + 5-formyl-5,6,7,8-tetrahydromethanopterin ternary complex type mechanism Methanothermobacter thermautotrophicus
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.101 539
-
-
 Methanothermobacter thermautotrophicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.101 formylmethanofuran + 5,6,7,8-tetrahydromethanopterin equilibrium favors transfer of the formyl group to tetrahydromethanopterin at physiological pH Methanothermobacter thermautotrophicus methanofuran + N5-formyl-5,6,7,8-tetrahydromethanopterin
-
 r
2.3.1.101 formylmethanofuran + 5,6,7,8-tetrahydromethanopterin involved in the formation of methane from CO2 Methanothermobacter thermautotrophicus methanofuran + N5-formyl-5,6,7,8-tetrahydromethanopterin
-
 r

Subunits

EC Number Subunits Comment Organism
2.3.1.101 tetramer 4 * 41000, SDS-PAGE before and after treatment with dimethylsuberimidate Methanothermobacter thermautotrophicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.101 60
-
 assay at Methanothermobacter thermautotrophicus