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Literature summary extracted from

  • Sonoda, T.; Tatibana, M.
    Purification of N-acetyl-L-glutamate synthetase from rat liver mitochondria and substrate and activator specificity of the enzyme (1983), J. Biol. Chem., 258, 9839-9844.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.1 arginine 5.1fold activiation of matrix enzyme Rattus norvegicus
2.3.1.1 clupein
-
 Rattus norvegicus
2.3.1.1 L-argininic acid activates to a lesser extent than L-arginine Rattus norvegicus
2.3.1.1 Polyarginine
-
 Rattus norvegicus
2.3.1.1 polylysine
-
 Rattus norvegicus
2.3.1.1 salmin
-
 Rattus norvegicus

General Stability

EC Number General Stability Organism
2.3.1.1 Triton X-100 stabilizes Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.1 0.76
-
 acetyl-CoA
-
 Rattus norvegicus
2.3.1.1 3.7
-
 L-glutamate
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.1 57000
-
 3 * 57000, SDS-PAGE Rattus norvegicus
2.3.1.1 160000
-
 gel filtration, sucrose density gradient centrifugation Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.1 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.1 ammonium sulfate, DEAE Biogel, Sephacryl S-200, aminoacetyl biogel A, affi-gel blue, sucrose gradient, isoelectric focusing Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.1 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.1 92.4
-
 in the presence of 1 mM L-arginine Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.1 acetyl-CoA + DL-2-aminopimelate
-
 Rattus norvegicus CoA + 2-acetylaminoheptanedioate
-
 ?
2.3.1.1 acetyl-CoA + glycine
-
 Rattus norvegicus CoA + acetylaminoacetate
-
 ?
2.3.1.1 acetyl-CoA + L-2-aminoadipate DL-2-aminoadipate, 5.2% of activity with L-glutamate Rattus norvegicus CoA + 2-acetylaminohexanedioate
-
 ?
2.3.1.1 acetyl-CoA + L-glutamate
-
 Rattus norvegicus CoA + N-acetyl-L-glutamate
-
 ?
2.3.1.1 acetyl-CoA + L-glutamate-gamma-hydroxamate 15.5% of activity with glutamate Rattus norvegicus CoA + N-acetyl-L-glutamate-gamma-hydroxamate
-
 ?
2.3.1.1 acetyl-CoA + L-glutamine
-
 Rattus norvegicus ?
-
 ?
2.3.1.1 propionyl-CoA + L-glutamate
-
 Rattus norvegicus CoA + N-propionyl-L-glutamate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.1 trimer 3 * 57000, SDS-PAGE Rattus norvegicus