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Literature summary extracted from

  • Marvil, D.K.; Leisinger, T.
    N-Acetylglutamate synthase of Escherichia coli: purification, characterization, and molecular properties (1977), J. Biol. Chem., 252, 3295-3303.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.3.1.1 glycerol stabilizes Escherichia coli
2.3.1.1 L-arginine or N-acetyl-L-glutamate stabilizes the high molecular weight form of 300000 Da Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.1 arginine 0.02 mM, 50% inhibition; L-arginine Escherichia coli
2.3.1.1 Cd2+ 0.1 mM, 94% inhibition Escherichia coli
2.3.1.1 coenzyme A 2.5 mM, 50% inhibition Escherichia coli
2.3.1.1 Cu2+ 0.1 mM, 68% inhibition Escherichia coli
2.3.1.1 Hg2+ 0.1 mM; 50% inhibition Escherichia coli
2.3.1.1 high ionic strength
-
 Escherichia coli
2.3.1.1 KCl 100 mM, 36% inhibition after 1 h, 300 mM, 74% inhibition after 1 h, 500 mM, 87% inhibition after 1 h Escherichia coli
2.3.1.1 L-citrulline 10 mM, 75% inhibition Escherichia coli
2.3.1.1 N-acetylglutamate 25 mM, 50% inhibition Escherichia coli
2.3.1.1 N-ethylmaleimide
-
 Escherichia coli
2.3.1.1 p-chloromercuribenzoate
-
 Escherichia coli
2.3.1.1 p-hydroxymercuribenzoate
-
 Escherichia coli
2.3.1.1 Zn2+ 0.1 mM, 43% inhibition Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.1 51700
-
 6 * 51700, SDS-PAGE, cross-linking studies with dimethylsuberimidate, in the maximally aggregated state the enzyme exists as a hexamer Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.1 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.1 DEAE-cellulose, hydroxylapatite Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.1 133
-
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.1 acetyl-CoA + L-glutamate
-
 Escherichia coli CoA + N-acetyl-L-glutamate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.1 hexamer 6 * 51700, SDS-PAGE, cross-linking studies with dimethylsuberimidate, in the maximally aggregated state the enzyme exists as a hexamer Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.1 10
-
-
 Escherichia coli

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.3.1.1 7
-
 highest stability Escherichia coli