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Literature summary extracted from

  • Bystrykh, L.V.; Sokolov, A.P.; Trotsenko, Y.A.
    Purification and properties of dihydroxyacetone synthase from methylotrophic yeast Candida boidinii (1981), FEBS Lett., 132, 324-328.
No PubMed abstract available

General Stability

EC Number General Stability Organism
2.2.1.3 Ca2+ does not stabilize [Candida] boidinii
2.2.1.3 Mg2+ or NH4+ stabilizes for at least 80 h [Candida] boidinii

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.2.1.3 EDTA
-
 [Candida] boidinii
2.2.1.3 formaldehyde above 1 mM, xylulose 5-phosphate protects [Candida] boidinii
2.2.1.3 GSH
-
 [Candida] boidinii
2.2.1.3 NEM irreversible, GSH partially protects [Candida] boidinii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.2.1.3 Mg2+ 5 mM, essential for effective binding of thiamine diphosphate to enzyme molecule [Candida] boidinii
2.2.1.3 additional information no activation by Ca2+ [Candida] boidinii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.2.1.3 76000
-
 alpha2, 2 * 76000, SDS-PAGE [Candida] boidinii
2.2.1.3 145000
-
 gel filtration [Candida] boidinii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.2.1.3 D-xylulose 5-phosphate + formaldehyde [Candida] boidinii
-
 glyceraldehyde 3-phosphate + dihydroxyacetone i.e. 1,3-dihydroxy-2-propanone ir
2.2.1.3 D-xylulose 5-phosphate + formaldehyde [Candida] boidinii KD1
-
 glyceraldehyde 3-phosphate + dihydroxyacetone i.e. 1,3-dihydroxy-2-propanone ir

Organism

EC Number Organism UniProt Comment Textmining
2.2.1.3 [Candida] boidinii
-
-
-
2.2.1.3 [Candida] boidinii
-
 methanol grown
-
2.2.1.3 [Candida] boidinii KD1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.2.1.3
-
 [Candida] boidinii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.2.1.3 4
-
-
 [Candida] boidinii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.2.1.3 D-xylulose 5-phosphate + formaldehyde
-
 [Candida] boidinii glyceraldehyde 3-phosphate + dihydroxyacetone i.e. 1,3-dihydroxy-2-propanone ir
2.2.1.3 D-xylulose 5-phosphate + formaldehyde formaldehyde is the only acceptor [Candida] boidinii glyceraldehyde 3-phosphate + dihydroxyacetone i.e. 1,3-dihydroxy-2-propanone ir
2.2.1.3 D-xylulose 5-phosphate + formaldehyde no acceptors are glyceraldehyde, glyceraldehyde 3-phosphate, erythrose 4-phosphate, ribose 5-phosphate, glucose 6-phosphate, D-ribose, glutaraldehyde, dihydroxyacetone [Candida] boidinii glyceraldehyde 3-phosphate + dihydroxyacetone i.e. 1,3-dihydroxy-2-propanone ir
2.2.1.3 D-xylulose 5-phosphate + formaldehyde
-
 [Candida] boidinii KD1 glyceraldehyde 3-phosphate + dihydroxyacetone i.e. 1,3-dihydroxy-2-propanone ir
2.2.1.3 D-xylulose 5-phosphate + formaldehyde formaldehyde is the only acceptor [Candida] boidinii KD1 glyceraldehyde 3-phosphate + dihydroxyacetone i.e. 1,3-dihydroxy-2-propanone ir
2.2.1.3 D-xylulose 5-phosphate + formaldehyde no acceptors are glyceraldehyde, glyceraldehyde 3-phosphate, erythrose 4-phosphate, ribose 5-phosphate, glucose 6-phosphate, D-ribose, glutaraldehyde, dihydroxyacetone [Candida] boidinii KD1 glyceraldehyde 3-phosphate + dihydroxyacetone i.e. 1,3-dihydroxy-2-propanone ir
2.2.1.3 fructose 6-phosphate + formaldehyde
-
 [Candida] boidinii dihydroxyacetone + erythrose 4-phosphate
-
 ?
2.2.1.3 fructose 6-phosphate + formaldehyde
-
 [Candida] boidinii KD1 dihydroxyacetone + erythrose 4-phosphate
-
 ?
2.2.1.3 sedoheptulose 7-phosphate + formaldehyde
-
 [Candida] boidinii dihydroxyacetone + ribose
-
 ?
2.2.1.3 sedoheptulose 7-phosphate + formaldehyde
-
 [Candida] boidinii KD1 dihydroxyacetone + ribose
-
 ?

Subunits

EC Number Subunits Comment Organism
2.2.1.3 dimer alpha2, 2 * 76000, SDS-PAGE [Candida] boidinii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.2.1.3 additional information
-
-
 [Candida] boidinii
2.2.1.3 6.8 7.1
-
 [Candida] boidinii

Cofactor

EC Number Cofactor Comment Organism Structure
2.2.1.3 thiamine diphosphate tightly bound [Candida] boidinii
2.2.1.3 thiamine diphosphate requirement, thiamine diphosphate protein, 0.5 mM [Candida] boidinii