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Literature summary extracted from

  • Waites, M.J.; Quayle, J.R.
    The interrelation between transketolase and dihydroxyacetone synthase activities in the methylotrophic yeast Candida boidinii (1981), J. Gen. Microbiol., 124, 309-316.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.2.1.3 thiamine diphosphate, 0.005% w/v, stabilizes slightly [Candida] boidinii
2.2.1.3 unstable, formaldehyde, EDTA, ammonium sulfate, methanol, ethanol, DTT, xylulose 5-phosphate and PMSF do not stabilize [Candida] boidinii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.2.1.3 D-xylulose 5-phosphate + formaldehyde [Candida] boidinii
-
 glyceraldehyde 3-phosphate + dihydroxyacetone i.e. 1,3-dihydroxy-2-propanone ?

Organism

EC Number Organism UniProt Comment Textmining
2.2.1.3 [Candida] boidinii
-
 methanol grown
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.2.1.3 partial [Candida] boidinii

Reaction

EC Number Reaction Comment Organism Reaction ID
2.2.1.3 D-xylulose 5-phosphate + formaldehyde = D-glyceraldehyde 3-phosphate + glycerone mechanism (yeast, ), #1,2# <1-3, 5> [Candida] boidinii
a

Storage Stability

EC Number Storage Stability Organism
2.2.1.3 0°C, t1/2: 45 h [Candida] boidinii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.2.1.3 D-xylulose 5-phosphate + formaldehyde
-
 [Candida] boidinii glyceraldehyde 3-phosphate + dihydroxyacetone i.e. 1,3-dihydroxy-2-propanone ?
2.2.1.3 hydroxypyruvate + formaldehyde hydroxypyruvate can replace D-xylulose 5-phosphate with 35% [Candida] boidinii CO2 + dihydroxyacetone
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.2.1.3 20
-
 t1/2: about 100 min [Candida] boidinii
2.2.1.3 30
-
 t1/2: about 40 min [Candida] boidinii
2.2.1.3 35
-
 t1/2: about 10 min [Candida] boidinii
2.2.1.3 40
-
 t1/2: about 5 min [Candida] boidinii