Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Nilsson, U.; Meshalkina, L.; Lindqvist, Y.; Schneider, G.
    Examination of substrate binding in thiamin diphosphate-dependent transketolase by protein crystallography and site-directed mutagenesis (1997), J. Biol. Chem., 272, 1864-1869.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.2.1.1 cocrystallization of apotransketolase with 5 mM thiamine diphosphate, 5 mM CaCl2, 50 mM fructose-6-phosphate, 13-16% (w/W) polyethylenglycol 6000 in 50 mM glycyl-glycine buffer, pH 7.6, 0.0075 ml of a 20 mg/ml solution mixed with the same amount of mother liquid, space group: P212121 Saccharomyces cerevisiae

Protein Variants

EC Number Protein Variants Comment Organism
2.2.1.1 D477A lower activity than wild-type enzyme Saccharomyces cerevisiae
2.2.1.1 H469A lower activity than wild-type enzyme Saccharomyces cerevisiae
2.2.1.1 R359A lower activity than wild-type enzyme Saccharomyces cerevisiae
2.2.1.1 R528A lower activity than wild-type enzyme Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.2.1.1 0.0005
-
 thiamine diphosphate H469A Saccharomyces cerevisiae
2.2.1.1 0.0006
-
 thiamine diphosphate wild-type Saccharomyces cerevisiae
2.2.1.1 0.0012
-
 thiamine diphosphate D477A Saccharomyces cerevisiae
2.2.1.1 0.0019
-
 thiamine diphosphate R359A Saccharomyces cerevisiae
2.2.1.1 0.0024
-
 thiamine diphosphate R528A Saccharomyces cerevisiae
2.2.1.1 0.073
-
 D-xylulose 5-phosphate wild-type Saccharomyces cerevisiae
2.2.1.1 0.146
-
 D-ribose 5-phosphate wild-type Saccharomyces cerevisiae
2.2.1.1 0.163
-
 D-xylulose 5-phosphate R359A Saccharomyces cerevisiae
2.2.1.1 0.318
-
 D-xylulose 5-phosphate R528A Saccharomyces cerevisiae
2.2.1.1 0.595
-
 D-xylulose 5-phosphate D477A Saccharomyces cerevisiae
2.2.1.1 0.829
-
 D-xylulose 5-phosphate H469A Saccharomyces cerevisiae
2.2.1.1 1.75
-
 D-ribose 5-phosphate D477A Saccharomyces cerevisiae
2.2.1.1 5.65
-
 D-ribose 5-phosphate R359A Saccharomyces cerevisiae
2.2.1.1 5.97
-
 D-ribose 5-phosphate H469A Saccharomyces cerevisiae
2.2.1.1 7
-
 D-ribose 5-phosphate R528A Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.2.1.1 74200
-
 alpha2, 2 * 74200, SDS-PAGE Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
2.2.1.1 Saccharomyces cerevisiae P23254 baker's yeast
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.2.1.1 D-xylulose 5-phosphate + D-ribose 5-phosphate
-
 Saccharomyces cerevisiae sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
 r

Subunits

EC Number Subunits Comment Organism
2.2.1.1 dimer alpha2, 2 * 74200, SDS-PAGE Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.2.1.1 6.2 7.8 R359A, R528A Saccharomyces cerevisiae
2.2.1.1 6.6 7.8 H469A Saccharomyces cerevisiae
2.2.1.1 6.8 7.8 D477A Saccharomyces cerevisiae
2.2.1.1 7.6 8.2 wild-type Saccharomyces cerevisiae