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Literature summary extracted from

  • Philippov, P.P.; Shestakova, I.K.; Tikhomirova, N.K.; Kochetov, G.A.
    Characterization and properties of pig liver transketolase (1980), Biochim. Biophys. Acta, 613, 359-369.
    View publication on PubMed

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.2.1.1 additional information
-
 Sus scrofa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.2.1.1 30000
-
 alpha2beta2, 2 * 30000 + 2 * 54000, pretreated with 1% SDS, gel filtration in the presence of 0.1% SDS Sus scrofa
2.2.1.1 54000
-
 alpha2beta2, 2 * 30000 + 2 * 54000, pretreated with 1% SDS, gel filtration in the presence of 0.1% SDS Sus scrofa
2.2.1.1 138000
-
 meniscus depletion method Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
2.2.1.1 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.2.1.1
-
 Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.2.1.1 liver
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.2.1.1 0.88
-
-
 Sus scrofa

Storage Stability

EC Number Storage Stability Organism
2.2.1.1 -20°C, several days Sus scrofa

Subunits

EC Number Subunits Comment Organism
2.2.1.1 tetramer alpha2beta2, 2 * 30000 + 2 * 54000, pretreated with 1% SDS, gel filtration in the presence of 0.1% SDS Sus scrofa
2.2.1.1 tetramer alpha2beta2, 2 * 27000-29000 + 2 * 52000-56000, SDS-PAGE Sus scrofa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.2.1.1 20
-
 assay at Sus scrofa
2.2.1.1 30
-
 assay at Sus scrofa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.2.1.1 20
-
 at pH 6.5-8.7, 20 min stable Sus scrofa
2.2.1.1 40
-
 pH 8.0, activity hardly changes for 60 min Sus scrofa
2.2.1.1 50
-
 t1/2: 10 min Sus scrofa
2.2.1.1 50
-
 1 h stable in the presence of 4 mM thiamine diphosphate Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.2.1.1 additional information
-
 pI: 7.6-7.8 Sus scrofa
2.2.1.1 7.8 8.2
-
 Sus scrofa

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.2.1.1 5
-
 thiamine diphosphate dissociates from holoenzyme Sus scrofa
2.2.1.1 6.5 8.7 20°C, 20 min stable Sus scrofa

Cofactor

EC Number Cofactor Comment Organism Structure
2.2.1.1 thiamine diphosphate 2 mol thiamine diphosphate per mol enzyme Sus scrofa