EC Number | General Stability | Organism |
---|---|---|
2.1.3.7 | phosphate anions stabilize during purification | Streptomyces clavuligerus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.1.3.7 | (NH4)2SO4 | - |
Streptomyces clavuligerus | |
2.1.3.7 | adenosine-alpha,beta-methylene triphosphate | - |
Streptomyces clavuligerus | |
2.1.3.7 | adenosine-beta,gamma-methylene triphosphate | - |
Streptomyces clavuligerus | |
2.1.3.7 | ATP | above 1 mM | Streptomyces clavuligerus | |
2.1.3.7 | Ba2+ | weak | Streptomyces clavuligerus | |
2.1.3.7 | Cu2+ | - |
Streptomyces clavuligerus | |
2.1.3.7 | diphosphate | - |
Streptomyces clavuligerus | |
2.1.3.7 | Fe2+ | weak | Streptomyces clavuligerus | |
2.1.3.7 | NaCl | - |
Streptomyces clavuligerus | |
2.1.3.7 | Pb2+ | weak | Streptomyces clavuligerus | |
2.1.3.7 | Sn2+ | - |
Streptomyces clavuligerus | |
2.1.3.7 | Zn2+ | - |
Streptomyces clavuligerus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.1.3.7 | cytoplasm | - |
Streptomyces clavuligerus | 5737 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.1.3.7 | Mg2+ | activation, maximal activity in the presence of Mn2+ and ATP | Streptomyces clavuligerus | |
2.1.3.7 | Mn2+ | activation, maximal activity in the presence of Mg2+ and ATP | Streptomyces clavuligerus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.3.7 | carbamoyl phosphate + a 3-hydroxymethyl ceph-3-em-4-carboxylate | Streptomyces clavuligerus | catalyzes the synthesis of a wide range of carbamoyloxymethylcephems, e.g. cefuroxime | phosphate + a 3-carbamoyloxymethylcephem | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.1.3.7 | Streptomyces clavuligerus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.1.3.7 | - |
Streptomyces clavuligerus |
EC Number | Storage Stability | Organism |
---|---|---|
2.1.3.7 | -10°C, 50 mM phosphate buffer, pH 7.0, stable for several weeks | Streptomyces clavuligerus |
2.1.3.7 | 4°C, in the presence of ATP and carbamoyl phosphate, stable for at least 3 days | Streptomyces clavuligerus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.3.7 | carbamoyl phosphate + 7-aminodeacetylcephalosporanic acid | - |
Streptomyces clavuligerus | 7-beta-acylamino-3-carbamoyloxymethylcephem + phosphate | - |
? | |
2.1.3.7 | carbamoyl phosphate + 7beta-acylamino-3-hydroxymethylcephem | - |
Streptomyces clavuligerus | 7beta-acylamino-3-carbamoyloxymethylcephem + phosphate | - |
? | |
2.1.3.7 | carbamoyl phosphate + a 3-hydroxymethyl ceph-3-em-4-carboxylate | catalyzes the synthesis of a wide range of carbamoyloxymethylcephems, e.g. cefuroxime | Streptomyces clavuligerus | phosphate + a 3-carbamoyloxymethylcephem | - |
? | |
2.1.3.7 | carbamoyl phosphate + decarbamoylcefuroxime | - |
Streptomyces clavuligerus | carbamoylcefuroxime + phosphate | - |
? | |
2.1.3.7 | carbamoyl phosphate + [3-hydroxymethyl]deacetylcephalosporin C | ATP may be a substrate or an effector | Streptomyces clavuligerus | 7beta-(5-D-aminoadipamido)-3-carbamoyloxymethylceph-3-em-4-carboxylic acid + phosphate | - |
? |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.1.3.7 | 6.8 | - |
- |
Streptomyces clavuligerus |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.1.3.7 | 5.9 | 7.4 | about 75% of maximal activity at pH 5.9 and 7.4 | Streptomyces clavuligerus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.1.3.7 | ATP | activation, up to 1 mM, may be substrate or effector, maximal activity in the presence of Mg2+ and Mn2+ | Streptomyces clavuligerus | |
2.1.3.7 | dATP | activation, can replace ATP to some extent | Streptomyces clavuligerus |