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Literature summary extracted from

  • Roovers, M.; Sanchez, R.; Legrain, C.; Glansdorff, N.
    Experimental evolution of enzyme temperature activity profile: selection in vivo and characterization of low-temperature-adapted mutants of Pyrococcus furiosus ornithine carbamoyltransferase (2001), J. Bacteriol., 183, 1101-1105.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.3.3 expression of wild-type, Y227C, A240D, E277G, Y227C/E277G and A240D/E277G mutant enzyme in Saccharomyces cerevisiae Pyrococcus furiosus

Protein Variants

EC Number Protein Variants Comment Organism
2.1.3.3 A240D 10fold increase of Km at 55°C Pyrococcus furiosus
2.1.3.3 E277G 14fold increase of Km at 55°C Pyrococcus furiosus
2.1.3.3 Y227D slight increase of Km at 55°C Pyrococcus furiosus

Organism

EC Number Organism UniProt Comment Textmining
2.1.3.3 Pyrococcus furiosus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.3.3 recombinant enzyme, Mono Q, arginine-Sepharose column Pyrococcus furiosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.3.3 carbamoyl phosphate + L-ornithine
-
 Pyrococcus furiosus phosphate + L-citrulline
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.3.3 370
-
 ornithine at 30°C Pyrococcus furiosus
2.1.3.3 500
-
 ornithine at 55°C Pyrococcus furiosus
2.1.3.3 2200
-
 ornithine Y227C/E277G double mutant, at 30°C Pyrococcus furiosus
2.1.3.3 2900
-
 ornithine A240D/E277G double mutant, at 30°C Pyrococcus furiosus
2.1.3.3 3500
-
 ornithine Y227C/E277G double mutant, at 55°C Pyrococcus furiosus
2.1.3.3 4300
-
 ornithine A240D/E277G double mutant, at 55°C Pyrococcus furiosus