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Literature summary extracted from

  • Morizono, H.; Tuchman, M.; Rajagopal, B.S.; McCann, M.T.; Listrom, C.D.; Yuan, X.; Venugopal, D.; Barany, G.; Allewell, N.M.
    Expression, purification and kinetic characterization of wild-type human ornithine transcarbamylase and a recurrent mutant that produces 'late onset' hyperammonemia (1997), Biochem. J., 322, 625-631.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.1.3.3 additional information activity increases with ionic strength up to 100 mM Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.3.3 coexpression with GroES and GroEL in Escherichia coli Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.1.3.3 R277W shows no substrate inhibition by ornithine, 70fold lower affinity for L-ornithine Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.3.3 2-mercaptoethanol
-
 Homo sapiens
2.1.3.3 dithiothreitol
-
 Homo sapiens
2.1.3.3 ornithine recombinant wild-type enzyme Homo sapiens
2.1.3.3 Zn2+
-
 Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.1.3.3 Homo sapiens
-
 ornithine transcarbamoyltransferase deficiency is the most common cause of inherited urea cycle disorders
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.3.3 recombinant wild-type and R277W mutant enzyme, ammonium sulfate, delta-N-(phosphonacetyl)-L-ornithine affinity chromatography Homo sapiens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.3.3 40.2
-
 recombinant enzyme Homo sapiens

Storage Stability

EC Number Storage Stability Organism
2.1.3.3 -20°C, 20 mM Tris acetate, 2 mM EDTA, pH 7.4, 50% glycerol, no loss of activity Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.3.3 carbamoyl phosphate + L-ornithine
-
 Homo sapiens phosphate + L-citrulline
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.1.3.3 51
-
 R277W mutant enzyme, 50% loss of activity Homo sapiens
2.1.3.3 56
-
 recombinant enzyme, 50% loss of activity Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.3.3 8
-
 gradual loss of activity above pH 8.5 or below pH 6.5 Homo sapiens