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Literature summary extracted from

  • Ruepp, A.; Mueller, H.N.; Lottspeich, F.; Soppa, J.
    Catabolic ornithine transcarbamylase of Halobacterium halobium (salinarium): purification, characterization, sequence determination, and evolution (1995), J. Bacteriol., 177, 1129-1136.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.1.3.3 arginine 50 mM, 2fold activatin Halobacterium salinarum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.3.3
-
 Halobacterium salinarum

General Stability

EC Number General Stability Organism
2.1.3.3 unstable below 1 M KCl Halobacterium salinarum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.3.3 ADP 5 mM, 90% inhibition Halobacterium salinarum
2.1.3.3 AMP 5 mM, 90% inhibition Halobacterium salinarum
2.1.3.3 ATP 5 mM, 90% inhibition Halobacterium salinarum
2.1.3.3 CTP 5 mM, 90% inhibition Halobacterium salinarum
2.1.3.3 GTP 5 mM, 90% inhibition Halobacterium salinarum
2.1.3.3 phosphate slight inhibition Halobacterium salinarum
2.1.3.3 UTP 5 mM, 90% inhibition Halobacterium salinarum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.3.3 32000
-
 6 * 32000, deduced from nucleotide sequence Halobacterium salinarum
2.1.3.3 200000
-
 gel filtration Halobacterium salinarum

Organism

EC Number Organism UniProt Comment Textmining
2.1.3.3 Halobacterium salinarum Q48296 also known as Halobacterium salinarium, catabolic ornithine carbamoyltransferase
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.3.3 gel filtration, ammonium sulfate-mediated hydrophobic chromatography Halobacterium salinarum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.3.3 additional information
-
 highest activity at 4 M KCl Halobacterium salinarum

Storage Stability

EC Number Storage Stability Organism
2.1.3.3 25°C, high-salt solution, 1 year, no loss of activity Halobacterium salinarum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.3.3 carbamoyl phosphate + L-ornithine catabolic ornithine carbamoyltransferase, catalyzes the reverse reaction, i.e. the cleavage of citrulline Halobacterium salinarum phosphate + L-citrulline
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.3.3 hexamer 6 * 32000, deduced from nucleotide sequence Halobacterium salinarum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.3.3 8.8
-
 30% of maximal activity at pH 7.0 Halobacterium salinarum