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Literature summary extracted from

  • Templeton, M.D.; Sullivan, P.A.; Sheperd, M.G.
    The inhibition of ornithine transcarbamoylase from Escherichia coli W by phaseolotoxin (1984), Biochem. J., 224, 379-388.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.3.3 N-delta-(phosphosulfamyl)ornithylalanylhomoarginine trivial name phaseolotoxin, 0.1 mM, 93% inhibition Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.3.3 37500
-
 3 * 37500, SDS-PAGE Escherichia coli
2.1.3.3 95000
-
 gel filtration Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.1.3.3 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.3.3 N-delta-(phosphonoacetyl)ornithine affinity chromatography, Sephadex G-100 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.3.3 2914
-
-
 Escherichia coli

Storage Stability

EC Number Storage Stability Organism
2.1.3.3 -80°C, prolonged storage, no loss of activity Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.3.3 carbamoyl phosphate + L-ornithine
-
 Escherichia coli phosphate + L-citrulline
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.3.3 trimer 3 * 37500, SDS-PAGE Escherichia coli

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.1.3.3 0.0002
-
 N-delta-(phosphosulfamyl)ornithylalanylhomoarginine mixed or competitive vs. carbamoyl phosphate Escherichia coli
2.1.3.3 0.0009
-
 N-delta-(phosphosulfamyl)ornithylalanylhomoarginine noncompetitive vs. ornithine Escherichia coli