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Literature summary extracted from

  • Marshall, M.; Cohen, P.P.
    Ornithine transcarbamylase from Streptococcus faecalis and bovine liver. I. Isolation and subunit structure (1972), J. Biol. Chem., 247, 1641-1653.
    View publication on PubMed

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.3.3 37800
-
 3 * 37800, SDS-PAGE Bos taurus
2.1.3.3 39600
-
 6 * 39600, SDS-PAGE Enterococcus faecalis
2.1.3.3 108000
-
 sedimentation equilibrium measurement Bos taurus
2.1.3.3 223000
-
 sedimentation equilibrium measurement Enterococcus faecalis

Organism

EC Number Organism UniProt Comment Textmining
2.1.3.3 Bos taurus
-
-
-
2.1.3.3 Enterococcus faecalis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.3.3 ammonium sulfate Bos taurus
2.1.3.3 ammonium sulfate, streptomycin, DEAE-cellulose, heating, DEAE-Sephadex Enterococcus faecalis

Renatured (Commentary)

EC Number Renatured (Comment) Organism
2.1.3.3 effect of pH and temperature on the rate of renaturation of guanidine-HCl dissociated enzyme, approx. 90% recovery at 25°C and pH 7.0 Bos taurus
2.1.3.3 effect of pH and temperature on the rate of renaturation of guanidine-HCl dissociated enzyme, approx. 90% recovery at 25°C and pH 7.0 Enterococcus faecalis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.3.3 liver
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.3.3 780
-
-
 Bos taurus
2.1.3.3 3200
-
-
 Enterococcus faecalis

Storage Stability

EC Number Storage Stability Organism
2.1.3.3 enzyme suspension in 3.0 M ammonium sulfate, pH 5.0, 3 years, no loss of activity Enterococcus faecalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.3.3 carbamoyl phosphate + L-ornithine
-
 Bos taurus phosphate + L-citrulline
-
 ?
2.1.3.3 carbamoyl phosphate + L-ornithine
-
 Enterococcus faecalis phosphate + L-citrulline
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.3.3 hexamer 6 * 39600, SDS-PAGE Enterococcus faecalis
2.1.3.3 trimer 3 * 37800, SDS-PAGE Bos taurus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.1.3.3 9
-
 stable at low ionic strength, gradual loss of activity in 1 M KCl Enterococcus faecalis