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Literature summary extracted from

  • Khan, A.I.; Chowdhry, B.Z.; Yon, R.J.
    Wheat germ aspartate transcarbamoylase: revised purification, stability and re-evaluation of regulatory kinetics in terms of the Monod-Wyman-Changeux model (1999), Eur. J. Biochem., 259, 71-78.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.3.2 UMP
-
 Triticum aestivum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.3.2 0.023
-
 Carbamoyl phosphate in the absence of UMP Triticum aestivum
2.1.3.2 0.16
-
 L-aspartate
-
 Triticum aestivum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.3.2 carbamoylphosphate + L-aspartate Triticum aestivum
-
 phosphate + N-carbamoyl-L-aspartate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.3.2 Triticum aestivum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.3.2 ethanol, 10-carboxydecyl-Sepharose, DEAE-cellulose Triticum aestivum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.3.2 10.21
-
-
 Triticum aestivum

Storage Stability

EC Number Storage Stability Organism
2.1.3.2 4°C, 50 mM Tris-HCl, pH 7.5, 25% glycerol, 3 mM sodium azide, indefinitely, no loss of activity Triticum aestivum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.3.2 carbamoyl phosphate + L-aspartate
-
 Triticum aestivum phosphate + N-carbamoyl-L-aspartate
-
 ?
2.1.3.2 carbamoylphosphate + L-aspartate
-
 Triticum aestivum phosphate + N-carbamoyl-L-aspartate
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.3.2 47.2
-
 Carbamoyl phosphate
-
 Triticum aestivum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.1.3.2 0.00049
-
 UMP
-
 Triticum aestivum