Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Xu, Y.; Zhang, Y.; Liang, Z.; Van de Casteele, M.; Legrain, C.; Glansdorff, N.
    Aspartate carbamoyltransferase from a psychrophilic deep-sea bacterium, Vibrio strain 2693: properties of the enzyme, genetic organization and synthesis in Escherichia coli (1998), Microbiology, 144, 1435-1441.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.3.2 expression in Escherichia coli Vibrio sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.3.2 CTP
-
 Vibrio sp.
2.1.3.2 Phenylglyoxal
-
 Vibrio sp.
2.1.3.2 succinate
-
 Vibrio sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.3.2 0.3
-
 Carbamoyl phosphate
-
 Vibrio sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.3.2 300000
-
-
 Vibrio natriegens
2.1.3.2 320000
-
 gel filtration Vibrio sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.3.2 carbamoylphosphate + L-aspartate Vibrio sp.
-
 phosphate + N-carbamoyl-L-aspartate
-
 ?
2.1.3.2 carbamoylphosphate + L-aspartate Vibrio natriegens
-
 phosphate + N-carbamoyl-L-aspartate
-
 ?
2.1.3.2 carbamoylphosphate + L-aspartate Vibrio sp. 2693
-
 phosphate + N-carbamoyl-L-aspartate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.3.2 Vibrio natriegens
-
-
-
2.1.3.2 Vibrio sp.
-
 isolated from the deep atlantic
-
2.1.3.2 Vibrio sp. 2693
-
 isolated from the deep atlantic
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.3.2 native and recombinant enzyme Vibrio sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.3.2 carbamoyl phosphate + L-aspartate
-
 Vibrio sp. phosphate + N-carbamoyl-L-aspartate
-
 ?
2.1.3.2 carbamoyl phosphate + L-aspartate
-
 Vibrio natriegens phosphate + N-carbamoyl-L-aspartate
-
 ?
2.1.3.2 carbamoyl phosphate + L-aspartate
-
 Vibrio sp. 2693 phosphate + N-carbamoyl-L-aspartate
-
 ?
2.1.3.2 carbamoylphosphate + L-aspartate
-
 Vibrio sp. phosphate + N-carbamoyl-L-aspartate
-
 ?
2.1.3.2 carbamoylphosphate + L-aspartate
-
 Vibrio natriegens phosphate + N-carbamoyl-L-aspartate
-
 ?
2.1.3.2 carbamoylphosphate + L-aspartate
-
 Vibrio sp. 2693 phosphate + N-carbamoyl-L-aspartate
-
 ?

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.1.3.2 2 50 20% of maximal activity at 2°C, 30% at 6°C Vibrio sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.1.3.2 60
-
 more than 90% inactivation after 15 min, half-life: 4 min Vibrio sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.3.2 9 10
-
 Vibrio sp.