Literature summary extracted from

Adair, L.B.; Jones, M.E.
Purification and characteristics of aspartate transcarbamylase from Pseudomonas fluorescens (1972), J. Biol. Chem., 247, 2308-2315.

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
2.1.3.2	ATP	-	Pseudomonas fluorescens
2.1.3.2	CDP	-	Pseudomonas fluorescens
2.1.3.2	CTP	-	Pseudomonas fluorescens
2.1.3.2	diphosphate	-	Pseudomonas fluorescens
2.1.3.2	phosphate	-	Pseudomonas fluorescens
2.1.3.2	ribose-5-P	-	Pseudomonas fluorescens
2.1.3.2	UTP	-	Pseudomonas fluorescens

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
2.1.3.2	0.014	-	Carbamoyl phosphate	-	Pseudomonas fluorescens
2.1.3.2	2.75	-	L-aspartate	-	Pseudomonas fluorescens

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
2.1.3.2	180000	-	2 * 180000, SDS-PAGE	Pseudomonas fluorescens
2.1.3.2	360000	-	-	Pseudomonas fluorescens

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
2.1.3.2	carbamoylphosphate + L-aspartate	Pseudomonas fluorescens	-	phosphate + N-carbamoyl-L-aspartate	-	?

Organism

EC Number	Organism	UniProt	Comment	Textmining
2.1.3.2	Pseudomonas fluorescens	-	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
2.1.3.2	-	Pseudomonas fluorescens

Renatured (Commentary)

EC Number	Renatured (Comment)	Organism
2.1.3.2	dialysis after SDS-denaturation, 1% of original activity is restored	Pseudomonas fluorescens

Specific Activity [micromol/min/mg]

EC Number	Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
2.1.3.2	3.16	-	-	Pseudomonas fluorescens

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2.1.3.2	carbamoyl phosphate + L-aspartate	-	Pseudomonas fluorescens	phosphate + N-carbamoyl-L-aspartate	-	?
2.1.3.2	carbamoylphosphate + L-aspartate	-	Pseudomonas fluorescens	phosphate + N-carbamoyl-L-aspartate	-	?

Subunits

EC Number	Subunits	Comment	Organism
2.1.3.2	dimer	2 * 180000, SDS-PAGE	Pseudomonas fluorescens

Temperature Stability [°C]

EC Number	Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
2.1.3.2	60	-	loss of approx. 60% activity after 20°C	Pseudomonas fluorescens

pH Optimum

EC Number	pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
2.1.3.2	8.5	-	-	Pseudomonas fluorescens

pH Range

EC Number	pH Minimum	pH Maximum	Comment	Organism
2.1.3.2	7.5	9.5	-	Pseudomonas fluorescens

Ki Value [mM]

EC Number	Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
2.1.3.2	0.0004	-	diphosphate	-	Pseudomonas fluorescens
2.1.3.2	0.007	-	CTP	-	Pseudomonas fluorescens
2.1.3.2	0.0085	-	ATP	-	Pseudomonas fluorescens
2.1.3.2	0.0085	-	UTP	-	Pseudomonas fluorescens
2.1.3.2	0.1	-	phosphate	-	Pseudomonas fluorescens
2.1.3.2	0.1	-	CDP	-	Pseudomonas fluorescens
2.1.3.2	1.5	-	ribose-5-P	-	Pseudomonas fluorescens

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Release 2023.1 (January 2023)