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Literature summary extracted from

  • Adair, L.B.; Jones, M.E.
    Aspartate carbamyltransferase (Pseudomonas fluorescens) (1978), Methods Enzymol., 51, 51-58.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.3.2 ATP
-
 Pseudomonas fluorescens
2.1.3.2 CDP
-
 Pseudomonas fluorescens
2.1.3.2 CTP
-
 Pseudomonas fluorescens
2.1.3.2 diphosphate
-
 Pseudomonas fluorescens
2.1.3.2 phosphate
-
 Pseudomonas fluorescens
2.1.3.2 ribose-5-P
-
 Pseudomonas fluorescens
2.1.3.2 UTP
-
 Pseudomonas fluorescens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.3.2 180000
-
 2 * 180000, SDS-PAGE Pseudomonas fluorescens
2.1.3.2 360000
-
 sedimentation equilibrium Pseudomonas fluorescens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.3.2 carbamoylphosphate + L-aspartate Pseudomonas fluorescens
-
 phosphate + N-carbamoyl-L-aspartate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.3.2 Pseudomonas fluorescens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.3.2 Streptomycin sulfate, ammonium sulfate, Sephadex G-200, hydroxylapatite, preparative gel electrophoresis Pseudomonas fluorescens

Renatured (Commentary)

EC Number Renatured (Comment) Organism
2.1.3.2 dialysis after SDS-denaturation, 1% of original activity is restored Pseudomonas fluorescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.3.2 carbamoyl phosphate + L-aspartate
-
 Pseudomonas fluorescens phosphate + N-carbamoyl-L-aspartate
-
 ?
2.1.3.2 carbamoylphosphate + L-aspartate
-
 Pseudomonas fluorescens phosphate + N-carbamoyl-L-aspartate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.3.2 dimer 2 * 180000, SDS-PAGE Pseudomonas fluorescens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.1.3.2 0.0004
-
 diphosphate when carbamoyl phosphate is limiting Pseudomonas fluorescens
2.1.3.2 0.007
-
 CTP when carbamoyl phosphate is limiting Pseudomonas fluorescens
2.1.3.2 0.0085
-
 ATP when carbamoyl phosphate is limiting Pseudomonas fluorescens
2.1.3.2 0.0085
-
 UTP when carbamoyl phosphate is limiting Pseudomonas fluorescens
2.1.3.2 0.1
-
 phosphate when carbamoyl phosphate is limiting Pseudomonas fluorescens
2.1.3.2 0.1
-
 CDP when carbamoyl phosphate is limiting Pseudomonas fluorescens
2.1.3.2 1.5
-
 ribose-5-P when carbamoyl phosphate is limiting Pseudomonas fluorescens