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Literature summary extracted from

  • Jacobson, G.R.; Stark, G.R.
    Aspartate transcarbamylases (1973), The Enzymes, 3rd Ed. (Boyer, P. D. , ed. ), 9, 225-308.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.1.3.2 adenosine very weak activation Escherichia coli

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.1.3.2 X-ray structure at 5.5 A resolution Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.3.2 2-ThioUMP
-
 Escherichia coli
2.1.3.2 CTP 800-1000 mM urea lower or eliminate CTP inhibition Escherichia coli
2.1.3.2 CTP
-
 Halobacterium salinarum
2.1.3.2 CTP
-
 Pseudomonas aeruginosa
2.1.3.2 CTP
-
 Pseudomonas fluorescens
2.1.3.2 CTP
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.1.3.2 cytidine
-
 Escherichia coli
2.1.3.2 diphosphate
-
 Enterococcus faecalis
2.1.3.2 diphosphate
-
 Escherichia coli
2.1.3.2 diphosphate
-
 Pseudomonas fluorescens
2.1.3.2 GTP
-
 Escherichia coli
2.1.3.2 GTP
-
 Pseudomonas aeruginosa
2.1.3.2 GTP
-
 Vigna radiata var. radiata
2.1.3.2 Inosine
-
 Escherichia coli
2.1.3.2 ITP weak inhibition Escherichia coli
2.1.3.2 N-(Phosphonoacetyl)-L-aspartate
-
 Bacillus subtilis
2.1.3.2 N-(Phosphonoacetyl)-L-aspartate
-
 Escherichia coli
2.1.3.2 N-(Phosphonoacetyl)-L-aspartate
-
 Mus musculus
2.1.3.2 N-(Phosphonoacetyl)-L-aspartate
-
 Vigna radiata var. radiata
2.1.3.2 N-methyl phosphonoacetamide
-
 Escherichia coli
2.1.3.2 nucleotides
-
 Citrobacter freundii
2.1.3.2 nucleotides
-
 Escherichia coli
2.1.3.2 nucleotides
-
 Pseudomonas aeruginosa
2.1.3.2 nucleotides
-
 Pseudomonas fluorescens
2.1.3.2 phosphonoacetamide
-
 Escherichia coli
2.1.3.2 UTP weak inhibition Escherichia coli
2.1.3.2 UTP
-
 Pseudomonas aeruginosa
2.1.3.2 UTP
-
 Pseudomonas fluorescens
2.1.3.2 UTP
-
 Saccharomyces cerevisiae
2.1.3.2 UTP
-
 Vigna radiata var. radiata

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.3.2 Zn2+ regulatory subunit contains 6 Zn2+ per 300000 Da protein, Zn2+ may be replaced by Hg2+ or Cd2+ Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.3.2 93000
-
-
 Vigna radiata var. radiata
2.1.3.2 93000
-
 250000 Da and 93000 Da isoforms Citrobacter freundii
2.1.3.2 100000
-
 100000 Da and 300000 Da isofomrms Pseudomonas vulgaris
2.1.3.2 120000 140000
-
 Enterococcus faecalis
2.1.3.2 250000
-
 250000 Da and 93000 Da isoforms Citrobacter freundii
2.1.3.2 300000
-
 100000 Da and 300000 Da isoforms Salmonella enterica subsp. enterica serovar Typhimurium
2.1.3.2 300000
-
 100000 Da and 300000 Da isoforms Pseudomonas aeruginosa
2.1.3.2 300000
-
 100000 Da and 300000 Da isoforms Serratia marcescens
2.1.3.2 300000
-
 100000 Da and 300000 Da isoforms Azotobacter vinelandii
2.1.3.2 300000
-
 100000 Da and 300000 Da isoforms Pseudomonas vulgaris
2.1.3.2 310000
-
 sedimentation equilibrium Escherichia coli
2.1.3.2 360000
-
-
 Pseudomonas fluorescens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.3.2 carbamoylphosphate + L-aspartate Salmonella enterica subsp. enterica serovar Typhimurium
-
 phosphate + N-carbamoyl-L-aspartate
-
 ?
2.1.3.2 carbamoylphosphate + L-aspartate Vigna radiata var. radiata
-
 phosphate + N-carbamoyl-L-aspartate
-
 ?
2.1.3.2 carbamoylphosphate + L-aspartate Bacillus subtilis
-
 phosphate + N-carbamoyl-L-aspartate
-
 ?
2.1.3.2 carbamoylphosphate + L-aspartate Mus musculus
-
 phosphate + N-carbamoyl-L-aspartate
-
 ?
2.1.3.2 carbamoylphosphate + L-aspartate Rattus norvegicus
-
 phosphate + N-carbamoyl-L-aspartate
-
 ?
2.1.3.2 carbamoylphosphate + L-aspartate Saccharomyces cerevisiae
-
 phosphate + N-carbamoyl-L-aspartate
-
 ?
2.1.3.2 carbamoylphosphate + L-aspartate Triticum aestivum
-
 phosphate + N-carbamoyl-L-aspartate
-
 ?
2.1.3.2 carbamoylphosphate + L-aspartate Oryctolagus cuniculus
-
 phosphate + N-carbamoyl-L-aspartate
-
 ?
2.1.3.2 carbamoylphosphate + L-aspartate Neurospora crassa
-
 phosphate + N-carbamoyl-L-aspartate
-
 ?
2.1.3.2 carbamoylphosphate + L-aspartate Pseudomonas fluorescens
-
 phosphate + N-carbamoyl-L-aspartate
-
 ?
2.1.3.2 carbamoylphosphate + L-aspartate Klebsiella aerogenes
-
 phosphate + N-carbamoyl-L-aspartate
-
 ?
2.1.3.2 carbamoylphosphate + L-aspartate Pseudomonas aeruginosa
-
 phosphate + N-carbamoyl-L-aspartate
-
 ?
2.1.3.2 carbamoylphosphate + L-aspartate Halobacterium salinarum
-
 phosphate + N-carbamoyl-L-aspartate
-
 ?
2.1.3.2 carbamoylphosphate + L-aspartate Serratia marcescens
-
 phosphate + N-carbamoyl-L-aspartate
-
 ?
2.1.3.2 carbamoylphosphate + L-aspartate Enterococcus faecalis
-
 phosphate + N-carbamoyl-L-aspartate
-
 ?
2.1.3.2 carbamoylphosphate + L-aspartate Azotobacter vinelandii
-
 phosphate + N-carbamoyl-L-aspartate
-
 ?
2.1.3.2 carbamoylphosphate + L-aspartate Lactuca sativa
-
 phosphate + N-carbamoyl-L-aspartate
-
 ?
2.1.3.2 carbamoylphosphate + L-aspartate Citrobacter freundii
-
 phosphate + N-carbamoyl-L-aspartate
-
 ?
2.1.3.2 carbamoylphosphate + L-aspartate Pseudomonas vulgaris
-
 phosphate + N-carbamoyl-L-aspartate
-
 ?
2.1.3.2 carbamoylphosphate + L-aspartate Escherichia coli catalyzes the formation of carbamoyl-L-aspartate, the first compound unique to the biosynthetic pathway for pyrimidine nucleotides phosphate + N-carbamoyl-L-aspartate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.3.2 Azotobacter vinelandii
-
-
-
2.1.3.2 Bacillus subtilis
-
-
-
2.1.3.2 Citrobacter freundii
-
-
-
2.1.3.2 Enterococcus faecalis
-
-
-
2.1.3.2 Escherichia coli
-
-
-
2.1.3.2 Halobacterium salinarum
-
-
-
2.1.3.2 Klebsiella aerogenes
-
-
-
2.1.3.2 Lactuca sativa
-
-
-
2.1.3.2 Mus musculus
-
-
-
2.1.3.2 Neurospora crassa
-
-
-
2.1.3.2 Oryctolagus cuniculus
-
-
-
2.1.3.2 Pseudomonas aeruginosa
-
-
-
2.1.3.2 Pseudomonas fluorescens
-
-
-
2.1.3.2 Pseudomonas vulgaris
-
-
-
2.1.3.2 Rattus norvegicus
-
-
-
2.1.3.2 Saccharomyces cerevisiae
-
-
-
2.1.3.2 Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-
2.1.3.2 Serratia marcescens
-
-
-
2.1.3.2 Triticum aestivum
-
 wheat
-
2.1.3.2 Vigna radiata var. radiata
-
 mung bean
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.3.2
-
 Escherichia coli

Reaction

EC Number Reaction Comment Organism Reaction ID
2.1.3.2 carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate reaction mechanism Salmonella enterica subsp. enterica serovar Typhimurium
a
2.1.3.2 carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate reaction mechanism Vigna radiata var. radiata
a
2.1.3.2 carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate reaction mechanism Bacillus subtilis
a
2.1.3.2 carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate reaction mechanism Mus musculus
a
2.1.3.2 carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate reaction mechanism Escherichia coli
a
2.1.3.2 carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate reaction mechanism Rattus norvegicus
a
2.1.3.2 carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate reaction mechanism Saccharomyces cerevisiae
a
2.1.3.2 carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate reaction mechanism Triticum aestivum
a
2.1.3.2 carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate reaction mechanism Oryctolagus cuniculus
a
2.1.3.2 carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate reaction mechanism Neurospora crassa
a
2.1.3.2 carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate reaction mechanism Pseudomonas fluorescens
a
2.1.3.2 carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate reaction mechanism Klebsiella aerogenes
a
2.1.3.2 carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate reaction mechanism Pseudomonas aeruginosa
a
2.1.3.2 carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate reaction mechanism Halobacterium salinarum
a
2.1.3.2 carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate reaction mechanism Serratia marcescens
a
2.1.3.2 carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate reaction mechanism Enterococcus faecalis
a
2.1.3.2 carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate reaction mechanism Azotobacter vinelandii
a
2.1.3.2 carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate reaction mechanism Lactuca sativa
a
2.1.3.2 carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate reaction mechanism Citrobacter freundii
a
2.1.3.2 carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate reaction mechanism Pseudomonas vulgaris
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.3.2 erythrocyte
-
 Oryctolagus cuniculus
-
2.1.3.2 germ
-
 Vigna radiata var. radiata
-
2.1.3.2 germ
-
 Triticum aestivum
-
2.1.3.2 seedling
-
 Vigna radiata var. radiata
-
2.1.3.2 seedling
-
 Lactuca sativa
-
2.1.3.2 spleen
-
 Mus musculus
-
2.1.3.2 spleen
-
 Rattus norvegicus
-
2.1.3.2 spleen
-
 Oryctolagus cuniculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.3.2 carbamoyl phosphate + L-aspartate
-
 Salmonella enterica subsp. enterica serovar Typhimurium phosphate + N-carbamoyl-L-aspartate
-
 ?
2.1.3.2 carbamoyl phosphate + L-aspartate
-
 Vigna radiata var. radiata phosphate + N-carbamoyl-L-aspartate
-
 ?
2.1.3.2 carbamoyl phosphate + L-aspartate
-
 Bacillus subtilis phosphate + N-carbamoyl-L-aspartate
-
 ?
2.1.3.2 carbamoyl phosphate + L-aspartate
-
 Mus musculus phosphate + N-carbamoyl-L-aspartate
-
 ?
2.1.3.2 carbamoyl phosphate + L-aspartate
-
 Escherichia coli phosphate + N-carbamoyl-L-aspartate
-
 ?
2.1.3.2 carbamoyl phosphate + L-aspartate
-
 Rattus norvegicus phosphate + N-carbamoyl-L-aspartate
-
 ?
2.1.3.2 carbamoyl phosphate + L-aspartate
-
 Saccharomyces cerevisiae phosphate + N-carbamoyl-L-aspartate
-
 ?
2.1.3.2 carbamoyl phosphate + L-aspartate
-
 Triticum aestivum phosphate + N-carbamoyl-L-aspartate
-
 ?
2.1.3.2 carbamoyl phosphate + L-aspartate
-
 Oryctolagus cuniculus phosphate + N-carbamoyl-L-aspartate
-
 ?
2.1.3.2 carbamoyl phosphate + L-aspartate
-
 Neurospora crassa phosphate + N-carbamoyl-L-aspartate
-
 ?
2.1.3.2 carbamoyl phosphate + L-aspartate
-
 Pseudomonas fluorescens phosphate + N-carbamoyl-L-aspartate
-
 ?
2.1.3.2 carbamoyl phosphate + L-aspartate
-
 Klebsiella aerogenes phosphate + N-carbamoyl-L-aspartate
-
 ?
2.1.3.2 carbamoyl phosphate + L-aspartate
-
 Pseudomonas aeruginosa phosphate + N-carbamoyl-L-aspartate
-
 ?
2.1.3.2 carbamoyl phosphate + L-aspartate
-
 Halobacterium salinarum phosphate + N-carbamoyl-L-aspartate
-
 ?
2.1.3.2 carbamoyl phosphate + L-aspartate
-
 Serratia marcescens phosphate + N-carbamoyl-L-aspartate
-
 ?
2.1.3.2 carbamoyl phosphate + L-aspartate
-
 Enterococcus faecalis phosphate + N-carbamoyl-L-aspartate
-
 ?
2.1.3.2 carbamoyl phosphate + L-aspartate
-
 Azotobacter vinelandii phosphate + N-carbamoyl-L-aspartate
-
 ?
2.1.3.2 carbamoyl phosphate + L-aspartate
-
 Lactuca sativa phosphate + N-carbamoyl-L-aspartate
-
 ?
2.1.3.2 carbamoyl phosphate + L-aspartate
-
 Citrobacter freundii phosphate + N-carbamoyl-L-aspartate
-
 ?
2.1.3.2 carbamoyl phosphate + L-aspartate
-
 Pseudomonas vulgaris phosphate + N-carbamoyl-L-aspartate
-
 ?
2.1.3.2 carbamoylphosphate + L-aspartate
-
 Salmonella enterica subsp. enterica serovar Typhimurium phosphate + N-carbamoyl-L-aspartate
-
 ?
2.1.3.2 carbamoylphosphate + L-aspartate
-
 Vigna radiata var. radiata phosphate + N-carbamoyl-L-aspartate
-
 ?
2.1.3.2 carbamoylphosphate + L-aspartate
-
 Bacillus subtilis phosphate + N-carbamoyl-L-aspartate
-
 ?
2.1.3.2 carbamoylphosphate + L-aspartate
-
 Mus musculus phosphate + N-carbamoyl-L-aspartate
-
 ?
2.1.3.2 carbamoylphosphate + L-aspartate
-
 Rattus norvegicus phosphate + N-carbamoyl-L-aspartate
-
 ?
2.1.3.2 carbamoylphosphate + L-aspartate
-
 Saccharomyces cerevisiae phosphate + N-carbamoyl-L-aspartate
-
 ?
2.1.3.2 carbamoylphosphate + L-aspartate
-
 Triticum aestivum phosphate + N-carbamoyl-L-aspartate
-
 ?
2.1.3.2 carbamoylphosphate + L-aspartate
-
 Oryctolagus cuniculus phosphate + N-carbamoyl-L-aspartate
-
 ?
2.1.3.2 carbamoylphosphate + L-aspartate
-
 Neurospora crassa phosphate + N-carbamoyl-L-aspartate
-
 ?
2.1.3.2 carbamoylphosphate + L-aspartate
-
 Pseudomonas fluorescens phosphate + N-carbamoyl-L-aspartate
-
 ?
2.1.3.2 carbamoylphosphate + L-aspartate
-
 Klebsiella aerogenes phosphate + N-carbamoyl-L-aspartate
-
 ?
2.1.3.2 carbamoylphosphate + L-aspartate
-
 Pseudomonas aeruginosa phosphate + N-carbamoyl-L-aspartate
-
 ?
2.1.3.2 carbamoylphosphate + L-aspartate
-
 Halobacterium salinarum phosphate + N-carbamoyl-L-aspartate
-
 ?
2.1.3.2 carbamoylphosphate + L-aspartate
-
 Serratia marcescens phosphate + N-carbamoyl-L-aspartate
-
 ?
2.1.3.2 carbamoylphosphate + L-aspartate
-
 Enterococcus faecalis phosphate + N-carbamoyl-L-aspartate
-
 ?
2.1.3.2 carbamoylphosphate + L-aspartate
-
 Azotobacter vinelandii phosphate + N-carbamoyl-L-aspartate
-
 ?
2.1.3.2 carbamoylphosphate + L-aspartate
-
 Lactuca sativa phosphate + N-carbamoyl-L-aspartate
-
 ?
2.1.3.2 carbamoylphosphate + L-aspartate
-
 Citrobacter freundii phosphate + N-carbamoyl-L-aspartate
-
 ?
2.1.3.2 carbamoylphosphate + L-aspartate
-
 Pseudomonas vulgaris phosphate + N-carbamoyl-L-aspartate
-
 ?
2.1.3.2 carbamoylphosphate + L-aspartate catalyzes the formation of carbamoyl-L-aspartate, the first compound unique to the biosynthetic pathway for pyrimidine nucleotides Escherichia coli phosphate + N-carbamoyl-L-aspartate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.3.2 More
-
 Serratia marcescens
2.1.3.2 More 2 distinct kinds of separable subunits, a catalytically active C subunit of 33000 Da and a regulatory R subunit of 17000 Da, native structure: (C3)2(R2)3 i.e. a dimer of C subunits and a trimer of R subunits Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.3.2 8.5
-
-
 Bacillus subtilis
2.1.3.2 8.5
-
-
 Pseudomonas fluorescens
2.1.3.2 8.5
-
 maxima at pH 8.5 and pH 10.2 Escherichia coli
2.1.3.2 10.2
-
-
 Vigna radiata var. radiata
2.1.3.2 10.2
-
 maxima at pH 8.5 and pH 10.2 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.3.2 ATP activates Escherichia coli
2.1.3.2 dATP stimulates Escherichia coli

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.1.3.2 0.000027
-
 N-(phosphonacetyl)-L-aspartate
-
 Escherichia coli
2.1.3.2 0.037
-
 CTP
-
 Escherichia coli
2.1.3.2 0.09
-
 diphosphate
-
 Escherichia coli
2.1.3.2 0.66
-
 phosphonoacetamide
-
 Escherichia coli
2.1.3.2 1.54
-
 N-methyl phosphonoacetamide
-
 Escherichia coli