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Literature summary extracted from

  • Vial, L.; Gomez, P.; Panvert, M.; Schmitt, E.; Blanquet, S.; Mechulam, Y.
    Mitochondrial methionyl-tRNAfMet formyltransferase from Saccharomyces cerevisiae: Gene disruption and tRNA substrate specificity (2003), Biochemistry, 42, 932-939.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.2.9 cloning and expression of the gene encoding mitochondrial enzyme in Escherichia coli Pal13Tr-pMAF and in yeast, several strains lacking gene exhibit in minimal medium a thermosensitive respiratory growth and a slower growth rate Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.2.9 additional information
-
 additional information kinetic parameters of the formylation of various mutant methionyl-tRNAs Saccharomyces cerevisiae
2.1.2.9 0.0003
-
 E. coli L-methionyl-tRNAfMet
-
 Saccharomyces cerevisiae
2.1.2.9 0.0003
-
 E. coli L-isoleucyl-tRNAfMet
-
 Saccharomyces cerevisiae
2.1.2.9 0.0005
-
 E. coli L-valyl-tRNAfMet
-
 Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.1.2.9 mitochondrion
-
 Saccharomyces cerevisiae 5739
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.2.9 10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O Saccharomyces cerevisiae initiation of protein synthesis in mitochondria tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.2.9 Saccharomyces cerevisiae
-
 strains CMY214, W303, YPALS, DBY2057
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.2.9 purification of recombinant enzyme, expressed in Escherichia coli Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.2.9 10-formyltetrahydrofolate + E. coli L-isoleucyl-tRNAfMet
-
 Saccharomyces cerevisiae tetrahydrofolate + E. coli N-formylisoleucyl-tRNAfMet
-
 r
2.1.2.9 10-formyltetrahydrofolate + E. coli L-methionyl-tRNAfMet
-
 Saccharomyces cerevisiae tetrahydrofolate + E. coli N-formylmethionyl-tRNAfMet
-
 r
2.1.2.9 10-formyltetrahydrofolate + E. coli L-methionyl-tRNAfMet
-
 Saccharomyces cerevisiae tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 r
2.1.2.9 10-formyltetrahydrofolate + E. coli L-valyl-tRNAfMet
-
 Saccharomyces cerevisiae tetrahydrofolate + E. coli N-formylvalyl-tRNAfMet
-
 r
2.1.2.9 10-formyltetrahydrofolate + E. coli L-valyl-tRNAfMet
-
 Saccharomyces cerevisiae tetrahydrofolate + N-formylvalyl-tRNAfMet
-
 r
2.1.2.9 10-formyltetrahydrofolate + isoleucyl-tRNAfMet + H2O mischarged E. coli tRNAfMet Saccharomyces cerevisiae tetrahydrofolate + N-formylisoleucyl-tRNAfMet
-
 ?
2.1.2.9 10-formyltetrahydrofolate + isoleucyl-tRNAfMet + H2O 250fold reduced catalytic efficiency compared with methionyl-tRNAfMet Saccharomyces cerevisiae tetrahydrofolate + N-formylisoleucyl-tRNAfMet
-
 ?
2.1.2.9 10-formyltetrahydrofolate + L-isoleucyl-tRNAfMet
-
 Saccharomyces cerevisiae tetrahydrofolate + N-formylisoleucyl-tRNAfMet
-
 r
2.1.2.9 10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O formylates yeast mitochondrial methionyl-tRNAfMet, specific for initiator tRNAs Saccharomyces cerevisiae tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
2.1.2.9 10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O formylates methionyl-tRNAfMet from E. coli Saccharomyces cerevisiae tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
2.1.2.9 10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O enzyme recognizes nucleotidic features in the acceptor stem of mitochondrial initiator tRNA, recognition mechanism Saccharomyces cerevisiae tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
2.1.2.9 10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O initiation of protein synthesis in mitochondria Saccharomyces cerevisiae tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
2.1.2.9 10-formyltetrahydrofolate + valyl-tRNAfMet + H2O mischarged E. coli tRNAfMet Saccharomyces cerevisiae tetrahydrofolate + N-formylvalyl-tRNAfMet
-
 ?
2.1.2.9 10-formyltetrahydrofolate + valyl-tRNAfMet + H2O 25fold reduced catalytic efficiency compared with methionyl-tRNAfMet Saccharomyces cerevisiae tetrahydrofolate + N-formylvalyl-tRNAfMet
-
 ?
2.1.2.9 additional information mutant tRNAs as poor substrates Saccharomyces cerevisiae ?
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.2.9 additional information
-
 additional information kinetic parameters of the formylation of various mutant methionyl-tRNAs Saccharomyces cerevisiae
2.1.2.9 0.0005
-
 L-isoleucyl-tRNAfMet
-
 Saccharomyces cerevisiae
2.1.2.9 0.009
-
 L-valyl-tRNAfMet
-
 Saccharomyces cerevisiae
2.1.2.9 0.13
-
 E. coli L-methionyl-tRNAfMet
-
 Saccharomyces cerevisiae