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Literature summary extracted from

  • Li, Y.; Ramesh, V.; Mangroo, D.; Taneja, C.; RajBhandary, U.L.
    Suppressor mutations in Escherichia coli methionyl-tRNA formyltransferase that compensate for the formylation defect of a mutant tRNA aminoacylated with lysine (2000), Biochemistry, 39, 8039-8046.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.2.9 wild-type and mutant fmt gene encoding MTF is cloned, overexpression of wild-type enzyme in Escherichia coli Novablue Escherichia coli

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.1.2.9 crystal structure of enzyme complexed with formylmethionyl-tRNAfMet Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
2.1.2.9 A55T no suppressor mutation Escherichia coli
2.1.2.9 G178D suppressor mutation, compensates for the formylation defect of a mutant elongator lysine-tRNAmMet, it compensates for lysine that is attached to the tRNA, G178D, G178K and G178L are progressively weaker suppressors than G178E Escherichia coli
2.1.2.9 G178E suppressor mutation, compensates for the formylation defect of a mutant elongator lysine-tRNAmMet, G178D, G178K and G178L are progressively weaker suppressors Escherichia coli
2.1.2.9 G178K suppressor mutation, compensates for the formylation defect of a mutant elongator lysine-tRNAmMet, G178D, G178K and G178L are progressively weaker suppressors than G178E Escherichia coli
2.1.2.9 G178L suppressor mutation, compensates for the formylation defect of a mutant elongator lysine-tRNAmMet, G178D, G178K and G178L are progressively weaker suppressors than G178E Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.2.9 0.00051
-
 L-methionyl-tRNAfMet wild-type enzyme Escherichia coli
2.1.2.9 0.00072
-
 L-methionyl-tRNAfMet G178E mutant enzyme Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.2.9 10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O Escherichia coli involved in initiation of protein biosynthesis tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
2.1.2.9 10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O Escherichia coli JM109 involved in initiation of protein biosynthesis tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
2.1.2.9 10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O Escherichia coli CA274 involved in initiation of protein biosynthesis tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.2.9 Escherichia coli
-
-
-
2.1.2.9 Escherichia coli
-
 strains DH5Alpha, Novablue(DE3) pLysS
-
2.1.2.9 Escherichia coli CA274
-
-
-
2.1.2.9 Escherichia coli JM109
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.2.9 14fold purification of recombinant wild-type enzyme, overexpressed in Escherichia coli Novablue, purification of His-tagged G178E mutant enzyme Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.2.9 10-formyltetrahydrofolate + L-methionyl-tRNAfMet
-
 Escherichia coli tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 r
2.1.2.9 10-formyltetrahydrofolate + L-methionyl-tRNAfMet
-
 Escherichia coli JM109 tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 r
2.1.2.9 10-formyltetrahydrofolate + L-methionyl-tRNAfMet
-
 Escherichia coli CA274 tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 r
2.1.2.9 10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O specificity is restricted to methionyl-tRNAfMet Escherichia coli tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
2.1.2.9 10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O involved in initiation of protein biosynthesis Escherichia coli tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
2.1.2.9 10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O specificity is restricted to methionyl-tRNAfMet Escherichia coli JM109 tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
2.1.2.9 10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O involved in initiation of protein biosynthesis Escherichia coli JM109 tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
2.1.2.9 10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O specificity is restricted to methionyl-tRNAfMet Escherichia coli CA274 tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
2.1.2.9 10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O involved in initiation of protein biosynthesis Escherichia coli CA274 tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
2.1.2.9 additional information mutant tRNAs as poor substrates Escherichia coli ?
-
 ?
2.1.2.9 additional information enzyme structure Escherichia coli ?
-
 ?
2.1.2.9 additional information mutant tRNAs as poor substrates Escherichia coli JM109 ?
-
 ?
2.1.2.9 additional information enzyme structure Escherichia coli JM109 ?
-
 ?
2.1.2.9 additional information mutant tRNAs as poor substrates Escherichia coli CA274 ?
-
 ?
2.1.2.9 additional information enzyme structure Escherichia coli CA274 ?
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.2.9 8.9
-
 L-methionyl-tRNAfMet G178E mutant enzyme Escherichia coli
2.1.2.9 13.9
-
 L-methionyl-tRNAfMet wild-type enzyme Escherichia coli