EC Number | Cloned (Comment) | Organism |
---|---|---|
2.1.2.9 | fmt gene encoding MTF is cloned and sequenced, expression in Escherichia coli JM109 as His6-tagged enzyme | Escherichia coli |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
2.1.2.9 | - |
Escherichia coli |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.1.2.9 | G41D | no suppressor mutation, mutant is stably overproduced in Escherichia coli CA274 | Escherichia coli |
2.1.2.9 | G41K | suppressor mutation within a 16-amino acid insertion, compensates for the formylation defect of a U35A36/G72G73 mutant initiator tRNA, lacking a critical determinant in the acceptor stem | Escherichia coli |
2.1.2.9 | G41L | no suppressor mutation, mutant is stably overproduced in Escherichia coli CA274 | Escherichia coli |
2.1.2.9 | G41Q | no suppressor mutation, mutant is stably overproduced in Escherichia coli CA274 | Escherichia coli |
2.1.2.9 | G41R | suppressor mutation within a 16-amino acid insertion, compensates for the formylation defect of a U35A36/G72G73 mutant initiator tRNA, lacking a critical determinant in the acceptor stem | Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.1.2.9 | additional information | - |
additional information | values for wild-type and mutant enzyme with wild-type and mutant initiator tRNAs | Escherichia coli | |
2.1.2.9 | 0.00056 | - |
L-methionyl-tRNAfMet | - |
Escherichia coli |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.2.9 | 10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O | Escherichia coli | involved in initiation of protein biosynthesis | tetrahydrofolate + N-formylmethionyl-tRNAfMet | - |
? | |
2.1.2.9 | 10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O | Escherichia coli DH5-alphaF | involved in initiation of protein biosynthesis | tetrahydrofolate + N-formylmethionyl-tRNAfMet | - |
? | |
2.1.2.9 | 10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O | Escherichia coli JM109 | involved in initiation of protein biosynthesis | tetrahydrofolate + N-formylmethionyl-tRNAfMet | - |
? | |
2.1.2.9 | 10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O | Escherichia coli CA274 | involved in initiation of protein biosynthesis | tetrahydrofolate + N-formylmethionyl-tRNAfMet | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.1.2.9 | Escherichia coli | - |
- |
- |
2.1.2.9 | Escherichia coli | - |
strain DH5AlphaF' | - |
2.1.2.9 | Escherichia coli CA274 | - |
- |
- |
2.1.2.9 | Escherichia coli DH5-alphaF | - |
strain DH5AlphaF' | - |
2.1.2.9 | Escherichia coli JM109 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.1.2.9 | purification of wild-type enzyme and G41R, G41K mutant enzymes | Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.2.9 | 10-formyltetrahydrofolate + L-methionyl-tRNAfMet | - |
Escherichia coli | tetrahydrofolate + N-formylmethionyl-tRNAfMet | - |
r | |
2.1.2.9 | 10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O | recognition mechanism of methionyl-tRNAfMet | Escherichia coli | tetrahydrofolate + N-formylmethionyl-tRNAfMet | - |
? | |
2.1.2.9 | 10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O | important role of a 16-amino acid insertion loop in enzyme for tRNA recognition | Escherichia coli | tetrahydrofolate + N-formylmethionyl-tRNAfMet | - |
? | |
2.1.2.9 | 10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O | involved in initiation of protein biosynthesis | Escherichia coli | tetrahydrofolate + N-formylmethionyl-tRNAfMet | - |
? | |
2.1.2.9 | 10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O | recognition mechanism of methionyl-tRNAfMet | Escherichia coli DH5-alphaF | tetrahydrofolate + N-formylmethionyl-tRNAfMet | - |
? | |
2.1.2.9 | 10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O | important role of a 16-amino acid insertion loop in enzyme for tRNA recognition | Escherichia coli DH5-alphaF | tetrahydrofolate + N-formylmethionyl-tRNAfMet | - |
? | |
2.1.2.9 | 10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O | involved in initiation of protein biosynthesis | Escherichia coli DH5-alphaF | tetrahydrofolate + N-formylmethionyl-tRNAfMet | - |
? | |
2.1.2.9 | 10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O | recognition mechanism of methionyl-tRNAfMet | Escherichia coli JM109 | tetrahydrofolate + N-formylmethionyl-tRNAfMet | - |
? | |
2.1.2.9 | 10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O | important role of a 16-amino acid insertion loop in enzyme for tRNA recognition | Escherichia coli JM109 | tetrahydrofolate + N-formylmethionyl-tRNAfMet | - |
? | |
2.1.2.9 | 10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O | involved in initiation of protein biosynthesis | Escherichia coli JM109 | tetrahydrofolate + N-formylmethionyl-tRNAfMet | - |
? | |
2.1.2.9 | 10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O | recognition mechanism of methionyl-tRNAfMet | Escherichia coli CA274 | tetrahydrofolate + N-formylmethionyl-tRNAfMet | - |
? | |
2.1.2.9 | 10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O | important role of a 16-amino acid insertion loop in enzyme for tRNA recognition | Escherichia coli CA274 | tetrahydrofolate + N-formylmethionyl-tRNAfMet | - |
? | |
2.1.2.9 | 10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O | involved in initiation of protein biosynthesis | Escherichia coli CA274 | tetrahydrofolate + N-formylmethionyl-tRNAfMet | - |
? | |
2.1.2.9 | additional information | mutant tRNAs as poor substrates | Escherichia coli | ? | - |
? | |
2.1.2.9 | additional information | enzyme structure | Escherichia coli | ? | - |
? | |
2.1.2.9 | additional information | mutant tRNAs as poor substrates | Escherichia coli DH5-alphaF | ? | - |
? | |
2.1.2.9 | additional information | enzyme structure | Escherichia coli DH5-alphaF | ? | - |
? | |
2.1.2.9 | additional information | mutant tRNAs as poor substrates | Escherichia coli JM109 | ? | - |
? | |
2.1.2.9 | additional information | enzyme structure | Escherichia coli JM109 | ? | - |
? | |
2.1.2.9 | additional information | mutant tRNAs as poor substrates | Escherichia coli CA274 | ? | - |
? | |
2.1.2.9 | additional information | enzyme structure | Escherichia coli CA274 | ? | - |
? |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.1.2.9 | additional information | - |
additional information | values for wild-type and mutant enzyme with wild-type and mutant initiator tRNAs | Escherichia coli | |
2.1.2.9 | 41.5 | - |
L-methionyl-tRNAfMet | - |
Escherichia coli |