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Literature summary extracted from

  • Takeuchi, N.; Vial, L.; Panvert, M.; Schmitt, E.; Watanabe, K.; Mechulam, Y.; Blanquet, S.
    Recognition of tRNAs by methionyl-tRNA transformylase from mammalian mitochondria (2001), J. Biol. Chem., 276, 20064-20068.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.2.9 gene encoding mitochondrial enzyme is cloned and expressed in Escherichia coli BL21(DE3) as His-tagged enzyme Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.2.9 additional information
-
 additional information kinetic parameters of the formylation of various mutant methionyl-tRNAs Bos taurus
2.1.2.9 0.0003
-
 E. coli L-isoleucyl-tRNAfMet
-
 Bos taurus
2.1.2.9 0.0005
-
 E. coli L-methionyl-tRNAfMet
-
 Bos taurus
2.1.2.9 0.0014
-
 E. coli L-valyl-tRNAfMet
-
 Bos taurus
2.1.2.9 0.01
-
 E. coli L-methionyl-tRNAmMet above Bos taurus
2.1.2.9 0.01
-
 E. coli L-methionyl-tRNA1Val(CAU) above Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.1.2.9 mitochondrion
-
 Bos taurus 5739
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.2.9 additional information Bos taurus translational initiation in mitochondria ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.2.9 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.2.9 purification of His-tagged recombinant enzyme, expressed in Escherichia coli BL21(DE3) Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.2.9 liver
-
 Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.2.9 10-formyltetrahydrofolate + E. coli L-isoleucyl-tRNAfMet
-
 Bos taurus tetrahydrofolate + E. coli N-formylisoleucyl-tRNAfMet
-
 r
2.1.2.9 10-formyltetrahydrofolate + E. coli L-methionyl-tRNA1Val(CAU)
-
 Bos taurus tetrahydrofolate + E. coli N-formylisoleucyl-tRNA1Val(CAU)
-
 r
2.1.2.9 10-formyltetrahydrofolate + E. coli L-methionyl-tRNAfMet
-
 Bos taurus tetrahydrofolate + E. coli N-formylmethionyl-tRNAfMet
-
 r
2.1.2.9 10-formyltetrahydrofolate + E. coli L-methionyl-tRNAfMet
-
 Bos taurus tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 r
2.1.2.9 10-formyltetrahydrofolate + E. coli L-methionyl-tRNAmMet
-
 Bos taurus tetrahydrofolate + E. coli N-formylmethionyl-tRNAmMet
-
 r
2.1.2.9 10-formyltetrahydrofolate + E. coli L-methionyl-tRNAmMet
-
 Bos taurus tetrahydrofolate + N-formylmethionyl-tRNAmMet
-
 r
2.1.2.9 10-formyltetrahydrofolate + E. coli L-valyl-tRNAfMet
-
 Bos taurus tetrahydrofolate + E. coli N-formylvalyl-tRNAfMet
-
 r
2.1.2.9 10-formyltetrahydrofolate + isoleucyl-tRNAfMet + H2O mischarged E. coli tRNAfMet Bos taurus tetrahydrofolate + N-formylisoleucyl-tRNAfMet
-
 ?
2.1.2.9 10-formyltetrahydrofolate + isoleucyl-tRNAfMet + H2O less efficient than with methionyl-tRNAfMet Bos taurus tetrahydrofolate + N-formylisoleucyl-tRNAfMet
-
 ?
2.1.2.9 10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O formylates bovine mitochondrial and E. coli initiator Met-tRNA with equal efficiency, formylates E. coli elongator Met-tRNA at a lower rate Bos taurus tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
2.1.2.9 10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O enzyme preferentially recognizes the methionyl moiety of its tRNA substrate, small importance of the tRNA acceptor stem for recognition, E. coli tRNA mutants as substrates Bos taurus tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
2.1.2.9 10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O broader substrate specificity than that of E. coli enzyme Bos taurus tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
2.1.2.9 10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O recognition mechanism of methionyl-tRNAfMet Bos taurus tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
2.1.2.9 10-formyltetrahydrofolate + methionyl-tRNA1Val(CAU) + H2O mischarged E. coli tRNA1Val(CAU), less efficient than with methionyl-tRNAfMet Bos taurus tetrahydrofolate + N-formylmethionyl-tRNA1Val(CAU)
-
 ?
2.1.2.9 10-formyltetrahydrofolate + methionyl-tRNAmMet + H2O E. coli elongator Met-tRNAmMet, less efficient than with methionyl-tRNAfMet Bos taurus tetrahydrofolate + N-formylmethionyl-tRNAmMet
-
 ?
2.1.2.9 10-formyltetrahydrofolate + valyl-tRNAfMet + H2O mischarged E. coli tRNAfMet Bos taurus tetrahydrofolate + N-formylvalyl-tRNAfMet
-
 ?
2.1.2.9 10-formyltetrahydrofolate + valyl-tRNAfMet + H2O less efficiency than with methionyl-tRNAfMet Bos taurus tetrahydrofolate + N-formylvalyl-tRNAfMet
-
 ?
2.1.2.9 additional information no formylation of Val-tRNA1Met Bos taurus ?
-
 ?
2.1.2.9 additional information translational initiation in mitochondria Bos taurus ?
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.2.9 additional information
-
 additional information kinetic parameters of the formylation of various mutant methionyl-tRNAs Bos taurus
2.1.2.9 0.03
-
 E. coli L-methionyl-tRNA1Val(CAU) above Bos taurus
2.1.2.9 0.04
-
 E. coli L-methionyl-tRNAmMet above Bos taurus
2.1.2.9 0.93
-
 E. coli L-methionyl-tRNAfMet
-
 Bos taurus