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Literature summary extracted from

  • Takeuchi, N.; Ueda, T.; Watanabe, K.
    Expression and characterization of bovine mitochondrial methionyl-tRNA transformylase (1998), J. Biochem., 124, 1069-1071.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.2.9 gene encoding mitochondrial enzyme is cloned and expressed in Escherichia coli BL21(DE3) as His-tagged enzyme Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.2.9 0.00004
-
 bovine mitochondrial L-methionyl-tRNA
-
 Bos taurus
2.1.2.9 0.000097
-
 E. coli L-methionyl-tRNAmMet
-
 Bos taurus
2.1.2.9 0.0001
-
 E. coli L-methionyl-tRNAfMet
-
 Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.1.2.9 mitochondrion
-
 Bos taurus 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.2.9 40000
-
 x * 40000, SDS-PAGE Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.2.9 additional information Bos taurus translational initiation in mitochondria ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.2.9 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.2.9 purification of His-tagged recombinant enzyme, expressed in Escherichia coli BL21(DE3) Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.2.9 10-formyltetrahydrofolate + bovine mitochondrial L-methionyl-tRNA
-
 Bos taurus tetrahydrofolate + bovine mitochondrial N-formylmethionyl-tRNA
-
 r
2.1.2.9 10-formyltetrahydrofolate + E. coli L-methionyl-tRNAfMet
-
 Bos taurus tetrahydrofolate + E. coli N-formylmethionyl-tRNAfMet
-
 r
2.1.2.9 10-formyltetrahydrofolate + E. coli L-methionyl-tRNAmMet
-
 Bos taurus tetrahydrofolate + E. coli N-formylmethionyl-tRNAmMet
-
 r
2.1.2.9 10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O formylates bovine mitochondrial and E. coli initiator Met-tRNA with equal efficiency, formylates E. coli elongator Met-tRNA at a lower rate Bos taurus tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
2.1.2.9 10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O formylates methionyl-tRNAfMet from E. coli Bos taurus tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
2.1.2.9 10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O broader substrate specificity than that of E. coli enzyme Bos taurus tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
2.1.2.9 additional information translational initiation in mitochondria Bos taurus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.2.9 ? x * 40000, SDS-PAGE Bos taurus