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Literature summary extracted from

  • Giege, R.; Ebel, J.P.; Clarke, B.F.C.
    Formylation of mischarged E. coli tRNA Met f (1973), FEBS Lett., 30, 291-295.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.2.9 tRNAfMet non-aminoacylated tRNAfMet is a competitive inhibitor Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.2.9 0.0006
-
 L-methionyl-tRNAfMet
-
 Escherichia coli
2.1.2.9 0.0013
-
 phenylalanyl-tRNAfMet
-
 Escherichia coli
2.1.2.9 0.0014
-
 valyl-tRNAfMet
-
 Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.1.2.9 cytoplasm
-
 Escherichia coli 5737
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.2.9 10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O Escherichia coli involved in initiation of protein biosynthesis tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
2.1.2.9 10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O Escherichia coli K12 MO involved in initiation of protein biosynthesis tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.2.9 Escherichia coli
-
 lot 15-290
-
2.1.2.9 Escherichia coli K12 MO
-
 lot 15-290
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.2.9 10-formyltetrahydrofolate + L-methionyl-tRNAfMet
-
 Escherichia coli tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 r
2.1.2.9 10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O formylates methionyl-tRNAfMet from E. coli Escherichia coli tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
2.1.2.9 10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O specific for methionyl-tRNAfMet, specificity depends exclusively on the nature of tRNA moiety of aminoacylated tRNAfMet Escherichia coli tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
2.1.2.9 10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O involved in initiation of protein biosynthesis Escherichia coli tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
2.1.2.9 10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O formylates methionyl-tRNAfMet from E. coli Escherichia coli K12 MO tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
2.1.2.9 10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O specific for methionyl-tRNAfMet, specificity depends exclusively on the nature of tRNA moiety of aminoacylated tRNAfMet Escherichia coli K12 MO tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
2.1.2.9 10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O involved in initiation of protein biosynthesis Escherichia coli K12 MO tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
2.1.2.9 10-formyltetrahydrofolate + phenylalanyl-tRNAfMet + H2O mischarged E. coli tRNAfMet, reduced velocity compared to the formylation of methionyl-tRNAfMet Escherichia coli tetrahydrofolate + N-formylphenylalanyl-tRNAfMet
-
 ?
2.1.2.9 10-formyltetrahydrofolate + phenylalanyl-tRNAfMet + H2O mischarged E. coli tRNAfMet, reduced velocity compared to the formylation of methionyl-tRNAfMet Escherichia coli K12 MO tetrahydrofolate + N-formylphenylalanyl-tRNAfMet
-
 ?
2.1.2.9 10-formyltetrahydrofolate + valyl-tRNAfMet + H2O mischarged E. coli tRNAfMet Escherichia coli tetrahydrofolate + N-formylvalyl-tRNAfMet
-
 ?
2.1.2.9 10-formyltetrahydrofolate + valyl-tRNAfMet + H2O reduced velocity compared to the formylation of methionyl-tRNAfMet Escherichia coli tetrahydrofolate + N-formylvalyl-tRNAfMet
-
 ?
2.1.2.9 10-formyltetrahydrofolate + valyl-tRNAfMet + H2O mischarged E. coli tRNAfMet Escherichia coli K12 MO tetrahydrofolate + N-formylvalyl-tRNAfMet
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.2.9 37
-
 assay at Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.2.9 10-formyltetrahydrofolate
-
 Escherichia coli
2.1.2.9 tetrahydrofolate
-
 Escherichia coli