EC Number | Cloned (Comment) | Organism |
---|---|---|
2.1.2.8 | gene 42 encoding enzyme is sequenced and expressed in Escherichia coli | Tequatrovirus T4 |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
2.1.2.8 | crystal structure in the presence of substrate dCMP at 1.6 A resolution | Tequatrovirus T4 |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.1.2.8 | D179N | mutant with altered substrate preference, favoring dUMP rather than dCMP | Tequatrovirus T4 |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.1.2.8 | 28450 | - |
2 * 28450, homodimer of 246-residue subunits | Tequatrovirus T4 |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.2.8 | 5,10-methylenetetrahydrofolate + H2O + deoxycytidylate | Tequatrovirus T4 | - |
tetrahydrofolate + 5-hydroxymethyldeoxycytidylate | enzyme forms part of phage DNA protection system | ? | |
2.1.2.8 | 5,10-methylenetetrahydrofolate + H2O + deoxycytidylate | Tequatrovirus T4 | - |
tetrahydrofolate + 5-hydroxymethyldeoxycytidylate | 5-hydroxymethyl-dCMP is essential for DNA synthesis in phage T4 | ? | |
2.1.2.8 | 5,10-methylenetetrahydrofolate + H2O + deoxycytidylate | Tequatrovirus T4 | - |
tetrahydrofolate + 5-hydroxymethyldeoxycytidylate | involved in biosynthesis of DNA precursor | ? | |
2.1.2.8 | additional information | Tequatrovirus T4 | enzyme functions in vivo as a component of a multienzyme complex called dNTP synthetase | ? | - |
? | |
2.1.2.8 | additional information | Tequatrovirus T4 | enzyme is part of a complex efficiently channeling DNA precursors to the replication apparatus | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.1.2.8 | Tequatrovirus T4 | P08773 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.1.2.8 | purification of His6-tagged enzyme | Tequatrovirus T4 |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
2.1.2.8 | 5,10-methylenetetrahydrofolate + H2O + deoxycytidylate = tetrahydrofolate + 5-hydroxymethyldeoxycytidylate | catalytic mechanism | Tequatrovirus T4 |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.2.8 | 5,10-methylenetetrahydrofolate + H2O + deoxycytidylate | - |
Tequatrovirus T4 | tetrahydrofolate + 5-hydroxymethyldeoxycytidylate | enzyme forms part of phage DNA protection system | ? | |
2.1.2.8 | 5,10-methylenetetrahydrofolate + H2O + deoxycytidylate | - |
Tequatrovirus T4 | tetrahydrofolate + 5-hydroxymethyldeoxycytidylate | 5-hydroxymethyl-dCMP is essential for DNA synthesis in phage T4 | ? | |
2.1.2.8 | 5,10-methylenetetrahydrofolate + H2O + deoxycytidylate | - |
Tequatrovirus T4 | tetrahydrofolate + 5-hydroxymethyldeoxycytidylate | involved in biosynthesis of DNA precursor | ? | |
2.1.2.8 | 5,10-methylenetetrahydrofolate + H2O + deoxycytidylate | enzyme structure, dCMP binding site, folate cofactor binding site, way of substrate binding in the active site, key catalytic residue Cys-148 acts as nucleophile to attack the C6-atom of the cytosine base | Tequatrovirus T4 | tetrahydrofolate + 5-hydroxymethyldeoxycytidylate | - |
? | |
2.1.2.8 | additional information | enzyme functions in vivo as a component of a multienzyme complex called dNTP synthetase | Tequatrovirus T4 | ? | - |
? | |
2.1.2.8 | additional information | enzyme is part of a complex efficiently channeling DNA precursors to the replication apparatus | Tequatrovirus T4 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.1.2.8 | homodimer | 2 * 28450, homodimer of 246-residue subunits | Tequatrovirus T4 |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.1.2.8 | 5,10-methylenetetrahydrofolate | as cofactor | Tequatrovirus T4 | |
2.1.2.8 | 5,10-methylenetetrahydrofolate | weak binding of the folate cofactor to enzyme | Tequatrovirus T4 | |
2.1.2.8 | tetrahydrofolate | - |
Tequatrovirus T4 |