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Literature summary extracted from

  • Song, H.K.; Sohn, S.H.; Suh, S.W.
    Crystal structure of deoxycytidylate hydroxymethylase from bacteriophage T4, a component of the deoxyribonucleoside triphosphate-synthesizing complex (1999), EMBO J., 18, 1104-1113.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.2.8 gene 42 encoding enzyme is sequenced and expressed in Escherichia coli Tequatrovirus T4

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.1.2.8 crystal structure in the presence of substrate dCMP at 1.6 A resolution Tequatrovirus T4

Protein Variants

EC Number Protein Variants Comment Organism
2.1.2.8 D179N mutant with altered substrate preference, favoring dUMP rather than dCMP Tequatrovirus T4

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.2.8 28450
-
2 * 28450, homodimer of 246-residue subunits Tequatrovirus T4

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.2.8 5,10-methylenetetrahydrofolate + H2O + deoxycytidylate Tequatrovirus T4
-
tetrahydrofolate + 5-hydroxymethyldeoxycytidylate enzyme forms part of phage DNA protection system ?
2.1.2.8 5,10-methylenetetrahydrofolate + H2O + deoxycytidylate Tequatrovirus T4
-
tetrahydrofolate + 5-hydroxymethyldeoxycytidylate 5-hydroxymethyl-dCMP is essential for DNA synthesis in phage T4 ?
2.1.2.8 5,10-methylenetetrahydrofolate + H2O + deoxycytidylate Tequatrovirus T4
-
tetrahydrofolate + 5-hydroxymethyldeoxycytidylate involved in biosynthesis of DNA precursor ?
2.1.2.8 additional information Tequatrovirus T4 enzyme functions in vivo as a component of a multienzyme complex called dNTP synthetase ?
-
?
2.1.2.8 additional information Tequatrovirus T4 enzyme is part of a complex efficiently channeling DNA precursors to the replication apparatus ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.1.2.8 Tequatrovirus T4 P08773
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.2.8 purification of His6-tagged enzyme Tequatrovirus T4

Reaction

EC Number Reaction Comment Organism Reaction ID
2.1.2.8 5,10-methylenetetrahydrofolate + H2O + deoxycytidylate = tetrahydrofolate + 5-hydroxymethyldeoxycytidylate catalytic mechanism Tequatrovirus T4

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.2.8 5,10-methylenetetrahydrofolate + H2O + deoxycytidylate
-
Tequatrovirus T4 tetrahydrofolate + 5-hydroxymethyldeoxycytidylate enzyme forms part of phage DNA protection system ?
2.1.2.8 5,10-methylenetetrahydrofolate + H2O + deoxycytidylate
-
Tequatrovirus T4 tetrahydrofolate + 5-hydroxymethyldeoxycytidylate 5-hydroxymethyl-dCMP is essential for DNA synthesis in phage T4 ?
2.1.2.8 5,10-methylenetetrahydrofolate + H2O + deoxycytidylate
-
Tequatrovirus T4 tetrahydrofolate + 5-hydroxymethyldeoxycytidylate involved in biosynthesis of DNA precursor ?
2.1.2.8 5,10-methylenetetrahydrofolate + H2O + deoxycytidylate enzyme structure, dCMP binding site, folate cofactor binding site, way of substrate binding in the active site, key catalytic residue Cys-148 acts as nucleophile to attack the C6-atom of the cytosine base Tequatrovirus T4 tetrahydrofolate + 5-hydroxymethyldeoxycytidylate
-
?
2.1.2.8 additional information enzyme functions in vivo as a component of a multienzyme complex called dNTP synthetase Tequatrovirus T4 ?
-
?
2.1.2.8 additional information enzyme is part of a complex efficiently channeling DNA precursors to the replication apparatus Tequatrovirus T4 ?
-
?

Subunits

EC Number Subunits Comment Organism
2.1.2.8 homodimer 2 * 28450, homodimer of 246-residue subunits Tequatrovirus T4

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.2.8 5,10-methylenetetrahydrofolate as cofactor Tequatrovirus T4
2.1.2.8 5,10-methylenetetrahydrofolate weak binding of the folate cofactor to enzyme Tequatrovirus T4
2.1.2.8 tetrahydrofolate
-
Tequatrovirus T4