BRENDA - Enzyme Database

Kinetic and equilibrium alpha-secondary tritium isotope effects on reactions catalyzed by dCMP hydroxymethylase from bacteriophage T4

Graves, K.L.; Hardy, L.W.; Biochemistry 33, 13049-13056 (1994)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
2.1.2.8
structural gene 42 encodes enzyme
Escherichia virus T4
Engineering
EC Number
Amino acid exchange
Commentary
Organism
2.1.2.8
D179A
mutant not inactivated by 5-fluoro-dUMP
Escherichia virus T4
2.1.2.8
D179N
mutant with altered substrate preference, favoring dUMP rather than dCMP; mutation of active site, 10fold increased rate of inhibition by 5-fluoro-dUMP
Escherichia virus T4
2.1.2.8
D179S
mutant not inactivated by 5-fluoro-dUMP
Escherichia virus T4
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.1.2.8
5-fluorodeoxyuridylate
inactivates by formation of a covalent complex containing enzyme, inhibitor and methylenetetrahydrofolate in a time-dependent manner, 1 mol 5-fluoro-dUMP per enzyme monomer, no substantial inhibition of wild-type enzyme in vivo, inhibition mechanism
Escherichia virus T4
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.1.2.8
28600
-
x * 28600
Escherichia virus T4
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.1.2.8
5,10-methylenetetrahydrofolate + H2O + deoxycytidylate
Escherichia virus T4
-
tetrahydrofolate + 5-hydroxymethyldeoxycytidylate
5-hydroxymethyl-dCMP is essential for DNA synthesis in phage T4
Escherichia virus T4
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.1.2.8
Escherichia virus T4
-
Escherichia coli JM101 infected with bacteriophage T4
-
Reaction
EC Number
Reaction
Commentary
Organism
2.1.2.8
5,10-methylenetetrahydrofolate + H2O + deoxycytidylate = tetrahydrofolate + 5-hydroxymethyldeoxycytidylate
mechanism
Escherichia virus T4
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.1.2.8
5,10-methylenetetrahydrofolate + H2O + deoxycytidylate
-
485789
Escherichia virus T4
tetrahydrofolate + 5-hydroxymethyldeoxycytidylate
5-hydroxymethyl-dCMP is essential for DNA synthesis in phage T4
485789
Escherichia virus T4
?
2.1.2.8
5,10-methylenetetrahydrofolate + H2O + deoxycytidylate
Cys-148 is catalytically essential
485789
Escherichia virus T4
tetrahydrofolate + 5-hydroxymethyldeoxycytidylate
-
485789
Escherichia virus T4
r
Subunits
EC Number
Subunits
Commentary
Organism
2.1.2.8
?
x * 28600
Escherichia virus T4
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.1.2.8
5,10-methylenetetrahydrofolate
-
Escherichia virus T4
2.1.2.8
tetrahydrofolate
-
Escherichia virus T4
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.1.2.8
structural gene 42 encodes enzyme
Escherichia virus T4
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.1.2.8
5,10-methylenetetrahydrofolate
-
Escherichia virus T4
2.1.2.8
tetrahydrofolate
-
Escherichia virus T4
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
2.1.2.8
D179A
mutant not inactivated by 5-fluoro-dUMP
Escherichia virus T4
2.1.2.8
D179N
mutant with altered substrate preference, favoring dUMP rather than dCMP; mutation of active site, 10fold increased rate of inhibition by 5-fluoro-dUMP
Escherichia virus T4
2.1.2.8
D179S
mutant not inactivated by 5-fluoro-dUMP
Escherichia virus T4
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.1.2.8
5-fluorodeoxyuridylate
inactivates by formation of a covalent complex containing enzyme, inhibitor and methylenetetrahydrofolate in a time-dependent manner, 1 mol 5-fluoro-dUMP per enzyme monomer, no substantial inhibition of wild-type enzyme in vivo, inhibition mechanism
Escherichia virus T4
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.1.2.8
28600
-
x * 28600
Escherichia virus T4
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.1.2.8
5,10-methylenetetrahydrofolate + H2O + deoxycytidylate
Escherichia virus T4
-
tetrahydrofolate + 5-hydroxymethyldeoxycytidylate
5-hydroxymethyl-dCMP is essential for DNA synthesis in phage T4
Escherichia virus T4
?
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.1.2.8
5,10-methylenetetrahydrofolate + H2O + deoxycytidylate
-
485789
Escherichia virus T4
tetrahydrofolate + 5-hydroxymethyldeoxycytidylate
5-hydroxymethyl-dCMP is essential for DNA synthesis in phage T4
485789
Escherichia virus T4
?
2.1.2.8
5,10-methylenetetrahydrofolate + H2O + deoxycytidylate
Cys-148 is catalytically essential
485789
Escherichia virus T4
tetrahydrofolate + 5-hydroxymethyldeoxycytidylate
-
485789
Escherichia virus T4
r
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.1.2.8
?
x * 28600
Escherichia virus T4