BRENDA - Enzyme Database

On the inhibition of deoxycytidylate hydroxymethylase by 5-fluoro-2-deoxycytidine 5-monophosphate

Subramaniam, R.; Wang, Y.; Mathews, C.K.; Santi, D.V.; Arch. Biochem. Biophys. 275, 11-15 (1989)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.1.2.8
5-Fluoro-2'-deoxycytidine 5'-monophosphate
competitive inhibitor with respect to dCMP, not mechanism-based inhibitor
Escherichia virus T4
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.1.2.8
0.36
-
deoxycytidylate
-
Escherichia virus T4
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.1.2.8
5,10-methylenetetrahydrofolate + H2O + deoxycytidylate
Escherichia virus T4
-
tetrahydrofolate + 5-hydroxymethyldeoxycytidylate
enzyme is responsible for production of 5-hydroxymethyl-dCMP found in the DNA of the phage
Escherichia virus T4
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.1.2.8
Escherichia virus T4
-
Escherichia coli infected with bacteriophage T4
-
Purification (Commentary)
EC Number
Commentary
Organism
2.1.2.8
-
Escherichia virus T4
Reaction
EC Number
Reaction
Commentary
Organism
2.1.2.8
5,10-methylenetetrahydrofolate + H2O + deoxycytidylate = tetrahydrofolate + 5-hydroxymethyldeoxycytidylate
mechanism
Escherichia virus T4
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.1.2.8
1
-
-
Escherichia virus T4
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.1.2.8
5,10-methylenetetrahydrofolate + H2O + deoxycytidylate
-
485787
Escherichia virus T4
tetrahydrofolate + 5-hydroxymethyldeoxycytidylate
-
485787
Escherichia virus T4
?
2.1.2.8
5,10-methylenetetrahydrofolate + H2O + deoxycytidylate
-
485787
Escherichia virus T4
tetrahydrofolate + 5-hydroxymethyldeoxycytidylate
enzyme is responsible for production of 5-hydroxymethyl-dCMP found in the DNA of the phage
485787
Escherichia virus T4
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.1.2.8
25
-
assay at
Escherichia virus T4
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.1.2.8
5,10-methylenetetrahydrofolate
as cofactor
Escherichia virus T4
2.1.2.8
tetrahydrofolate
-
Escherichia virus T4
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.1.2.8
0.025
-
5-Fluoro-2'-deoxycytidine 5'-monophosphate
-
Escherichia virus T4
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.1.2.8
5,10-methylenetetrahydrofolate
as cofactor
Escherichia virus T4
2.1.2.8
tetrahydrofolate
-
Escherichia virus T4
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.1.2.8
5-Fluoro-2'-deoxycytidine 5'-monophosphate
competitive inhibitor with respect to dCMP, not mechanism-based inhibitor
Escherichia virus T4
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.1.2.8
0.025
-
5-Fluoro-2'-deoxycytidine 5'-monophosphate
-
Escherichia virus T4
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.1.2.8
0.36
-
deoxycytidylate
-
Escherichia virus T4
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.1.2.8
5,10-methylenetetrahydrofolate + H2O + deoxycytidylate
Escherichia virus T4
-
tetrahydrofolate + 5-hydroxymethyldeoxycytidylate
enzyme is responsible for production of 5-hydroxymethyl-dCMP found in the DNA of the phage
Escherichia virus T4
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.1.2.8
-
Escherichia virus T4
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.1.2.8
1
-
-
Escherichia virus T4
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.1.2.8
5,10-methylenetetrahydrofolate + H2O + deoxycytidylate
-
485787
Escherichia virus T4
tetrahydrofolate + 5-hydroxymethyldeoxycytidylate
-
485787
Escherichia virus T4
?
2.1.2.8
5,10-methylenetetrahydrofolate + H2O + deoxycytidylate
-
485787
Escherichia virus T4
tetrahydrofolate + 5-hydroxymethyldeoxycytidylate
enzyme is responsible for production of 5-hydroxymethyl-dCMP found in the DNA of the phage
485787
Escherichia virus T4
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.1.2.8
25
-
assay at
Escherichia virus T4