BRENDA - Enzyme Database

T4 phage-coded deoxycytidylate hydroxymethylase: purification and studies on intermolecular interactions

North, T.W.; Mathews, C.K.; Biochem. Biophys. Res. Commun. 77, 898-904 (1977)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
2.1.2.8
structural gene 42 encodes enzyme
Escherichia virus T4
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.1.2.8
additional information
enzyme is no membrane component or very loosely attached to the membrane
Escherichia virus T4
-
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.1.2.8
27000
-
2 * 27000, SDS-PAGE
Escherichia virus T4
2.1.2.8
57000
-
gel filtration
Escherichia virus T4
2.1.2.8
63000
-
sedimentation equilibrium method
Escherichia virus T4
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.1.2.8
5,10-methylenetetrahydrofolate + H2O + deoxycytidylate
Escherichia virus T4
-
tetrahydrofolate + 5-hydroxymethyldeoxycytidylate
5-hydroxymethyl-dCMP is a virus-specific DNA precursor
Escherichia virus T4
?
2.1.2.8
additional information
Escherichia virus T4
enzyme is part of a complex catalyzing DNA precursor biosynthesis
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.1.2.8
Escherichia virus T4
-
Escherichia coli B infected with bacteriophage T4D and amber mutants
-
Purification (Commentary)
EC Number
Commentary
Organism
2.1.2.8
Escherichia coli B infected with
Escherichia virus T4
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.1.2.8
4.9
-
-
Escherichia virus T4
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.1.2.8
5,10-methylenetetrahydrofolate + H2O + deoxycytidylate
-
485786
Escherichia virus T4
tetrahydrofolate + 5-hydroxymethyldeoxycytidylate
-
485786
Escherichia virus T4
?
2.1.2.8
5,10-methylenetetrahydrofolate + H2O + deoxycytidylate
-
485786
Escherichia virus T4
tetrahydrofolate + 5-hydroxymethyldeoxycytidylate
5-hydroxymethyl-dCMP is a virus-specific DNA precursor
485786
Escherichia virus T4
?
2.1.2.8
additional information
enzyme is part of a complex catalyzing DNA precursor biosynthesis
485786
Escherichia virus T4
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
2.1.2.8
dimer
2 * 27000, SDS-PAGE
Escherichia virus T4
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.1.2.8
5,10-methylenetetrahydrofolate
-
Escherichia virus T4
2.1.2.8
tetrahydrofolate
-
Escherichia virus T4
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.1.2.8
structural gene 42 encodes enzyme
Escherichia virus T4
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.1.2.8
5,10-methylenetetrahydrofolate
-
Escherichia virus T4
2.1.2.8
tetrahydrofolate
-
Escherichia virus T4
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.1.2.8
additional information
enzyme is no membrane component or very loosely attached to the membrane
Escherichia virus T4
-
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.1.2.8
27000
-
2 * 27000, SDS-PAGE
Escherichia virus T4
2.1.2.8
57000
-
gel filtration
Escherichia virus T4
2.1.2.8
63000
-
sedimentation equilibrium method
Escherichia virus T4
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.1.2.8
5,10-methylenetetrahydrofolate + H2O + deoxycytidylate
Escherichia virus T4
-
tetrahydrofolate + 5-hydroxymethyldeoxycytidylate
5-hydroxymethyl-dCMP is a virus-specific DNA precursor
Escherichia virus T4
?
2.1.2.8
additional information
Escherichia virus T4
enzyme is part of a complex catalyzing DNA precursor biosynthesis
?
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.1.2.8
Escherichia coli B infected with
Escherichia virus T4
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.1.2.8
4.9
-
-
Escherichia virus T4
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.1.2.8
5,10-methylenetetrahydrofolate + H2O + deoxycytidylate
-
485786
Escherichia virus T4
tetrahydrofolate + 5-hydroxymethyldeoxycytidylate
-
485786
Escherichia virus T4
?
2.1.2.8
5,10-methylenetetrahydrofolate + H2O + deoxycytidylate
-
485786
Escherichia virus T4
tetrahydrofolate + 5-hydroxymethyldeoxycytidylate
5-hydroxymethyl-dCMP is a virus-specific DNA precursor
485786
Escherichia virus T4
?
2.1.2.8
additional information
enzyme is part of a complex catalyzing DNA precursor biosynthesis
485786
Escherichia virus T4
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.1.2.8
dimer
2 * 27000, SDS-PAGE
Escherichia virus T4