BRENDA - Enzyme Database

Deoxycytidylate hydroxymethylase gene of bacteriophage T4. Nucleotide sequence determination and over-expression of the gene

Lamm, N.; Wang, Y.; Mathews, C.K.; Ruger, W.; Eur. J. Biochem. 172, 553-563 (1988)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
2.1.2.8
cloning and overexpression of g42 in Escherichia coli K38; nucleotide sequence of g42, the dCMP hydroxymethylase gene, encodes a protein of 246 amino acids
Escherichia virus T4
2.1.2.8
nucleotide sequence of dCMP hydroxymethylase gene encoding a protein of 246 amino acids
Enterobacteria phage T2
2.1.2.8
nucleotide sequence of dCMP hydroxymethylase gene encoding a protein of 246 amino acids
Enterobacteria phage T6
Engineering
EC Number
Amino acid exchange
Commentary
Organism
2.1.2.8
additional information
transition mutations in gene 42
Escherichia virus T4
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.1.2.8
27000
-
2 * 27000, SDS-PAGE
Escherichia virus T4
2.1.2.8
28450
-
calculation from the amino acid sequence
Enterobacteria phage T2
2.1.2.8
28450
-
calculation from the amino acid sequence
Enterobacteria phage T6
2.1.2.8
28450
-
calculation from the amino acid sequence
Escherichia virus T4
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.1.2.8
Enterobacteria phage T2
-
-
-
2.1.2.8
Enterobacteria phage T6
-
-
-
2.1.2.8
Escherichia virus T4
-
BK 536 and JSW 800, grown on Escherichia coli
-
Purification (Commentary)
EC Number
Commentary
Organism
2.1.2.8
-
Enterobacteria phage T6
2.1.2.8
-
Escherichia virus T4
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
2.1.2.8
5.865
-
-
Escherichia virus T4
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.1.2.8
5,10-methylenetetrahydrofolate + H2O + deoxycytidylate
-
485783
Escherichia virus T4
tetrahydrofolate + 5-hydroxymethyldeoxycytidylate
-
485783
Escherichia virus T4
?
2.1.2.8
5,10-methylenetetrahydrofolate + H2O + deoxycytidylate
-
485783
Enterobacteria phage T2
tetrahydrofolate + 5-hydroxymethyldeoxycytidylate
-
485783
Enterobacteria phage T2
?
2.1.2.8
5,10-methylenetetrahydrofolate + H2O + deoxycytidylate
-
485783
Enterobacteria phage T6
tetrahydrofolate + 5-hydroxymethyldeoxycytidylate
-
485783
Enterobacteria phage T6
?
Subunits
EC Number
Subunits
Commentary
Organism
2.1.2.8
dimer
2 * 27000, SDS-PAGE
Escherichia virus T4
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.1.2.8
additional information
-
additional information
333 mol substrate per min per 56.8 kg protein
Escherichia virus T4
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.1.2.8
5,10-methylenetetrahydrofolate
-
Enterobacteria phage T2
2.1.2.8
5,10-methylenetetrahydrofolate
-
Enterobacteria phage T6
2.1.2.8
5,10-methylenetetrahydrofolate
-
Escherichia virus T4
2.1.2.8
tetrahydrofolate
-
Enterobacteria phage T2
2.1.2.8
tetrahydrofolate
-
Enterobacteria phage T6
2.1.2.8
tetrahydrofolate
-
Escherichia virus T4
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.1.2.8
cloning and overexpression of g42 in Escherichia coli K38; nucleotide sequence of g42, the dCMP hydroxymethylase gene, encodes a protein of 246 amino acids
Escherichia virus T4
2.1.2.8
nucleotide sequence of dCMP hydroxymethylase gene encoding a protein of 246 amino acids
Enterobacteria phage T2
2.1.2.8
nucleotide sequence of dCMP hydroxymethylase gene encoding a protein of 246 amino acids
Enterobacteria phage T6
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.1.2.8
5,10-methylenetetrahydrofolate
-
Enterobacteria phage T2
2.1.2.8
5,10-methylenetetrahydrofolate
-
Enterobacteria phage T6
2.1.2.8
5,10-methylenetetrahydrofolate
-
Escherichia virus T4
2.1.2.8
tetrahydrofolate
-
Enterobacteria phage T2
2.1.2.8
tetrahydrofolate
-
Enterobacteria phage T6
2.1.2.8
tetrahydrofolate
-
Escherichia virus T4
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
2.1.2.8
additional information
transition mutations in gene 42
Escherichia virus T4
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.1.2.8
27000
-
2 * 27000, SDS-PAGE
Escherichia virus T4
2.1.2.8
28450
-
calculation from the amino acid sequence
Enterobacteria phage T2
2.1.2.8
28450
-
calculation from the amino acid sequence
Enterobacteria phage T6
2.1.2.8
28450
-
calculation from the amino acid sequence
Escherichia virus T4
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.1.2.8
-
Enterobacteria phage T6
2.1.2.8
-
Escherichia virus T4
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
2.1.2.8
5.865
-
-
Escherichia virus T4
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.1.2.8
5,10-methylenetetrahydrofolate + H2O + deoxycytidylate
-
485783
Escherichia virus T4
tetrahydrofolate + 5-hydroxymethyldeoxycytidylate
-
485783
Escherichia virus T4
?
2.1.2.8
5,10-methylenetetrahydrofolate + H2O + deoxycytidylate
-
485783
Enterobacteria phage T2
tetrahydrofolate + 5-hydroxymethyldeoxycytidylate
-
485783
Enterobacteria phage T2
?
2.1.2.8
5,10-methylenetetrahydrofolate + H2O + deoxycytidylate
-
485783
Enterobacteria phage T6
tetrahydrofolate + 5-hydroxymethyldeoxycytidylate
-
485783
Enterobacteria phage T6
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.1.2.8
dimer
2 * 27000, SDS-PAGE
Escherichia virus T4
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.1.2.8
additional information
-
additional information
333 mol substrate per min per 56.8 kg protein
Escherichia virus T4