BRENDA - Enzyme Database

Virus-induced acquisition of metabolic function. VII. Biosynthesis de novo of deoxycytidylate hydroxymethylase

Mathews, C.K.; Brown, F.; Cohen, S.S.; J. Biol. Chem. 239, 2957-2963 (1964)

Data extracted from this reference:

Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.1.2.8
68000
-
E. coli B infected with bacteriophage T6r+, gel filtration, sedimentation and diffusion data
bacteriophage T6r+
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.1.2.8
additional information
bacteriophage T6r+
biosynthesis of enzyme de novo after infection
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.1.2.8
bacteriophage T6r+
-
Escherichia coli strain B infected with bacteriophage T6r+
-
Purification (Commentary)
EC Number
Commentary
Organism
2.1.2.8
Escherichia coli infected with
bacteriophage T6r+
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.1.2.8
additional information
-
-
bacteriophage T6r+
Storage Stability
EC Number
Storage Stability
Organism
2.1.2.8
4°C, up to 10 days, only minor loss of activity
bacteriophage T6r+
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.1.2.8
5,10-methylenetetrahydrofolate + H2O + deoxycytidylate
-
485780
bacteriophage T6r+
tetrahydrofolate + 5-hydroxymethyldeoxycytidylate
-
485780
bacteriophage T6r+
?
2.1.2.8
additional information
biosynthesis of enzyme de novo after infection
485780
bacteriophage T6r+
?
-
-
-
-
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.1.2.8
additional information
-
additional information
320 substrate molecules per min per enzyme molecule
bacteriophage T6r+
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.1.2.8
5,10-methylenetetrahydrofolate
-
bacteriophage T6r+
2.1.2.8
tetrahydrofolate
-
bacteriophage T6r+
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.1.2.8
5,10-methylenetetrahydrofolate
-
bacteriophage T6r+
2.1.2.8
tetrahydrofolate
-
bacteriophage T6r+
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.1.2.8
68000
-
E. coli B infected with bacteriophage T6r+, gel filtration, sedimentation and diffusion data
bacteriophage T6r+
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.1.2.8
additional information
bacteriophage T6r+
biosynthesis of enzyme de novo after infection
?
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.1.2.8
Escherichia coli infected with
bacteriophage T6r+
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.1.2.8
additional information
-
-
bacteriophage T6r+
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.1.2.8
4°C, up to 10 days, only minor loss of activity
bacteriophage T6r+
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.1.2.8
5,10-methylenetetrahydrofolate + H2O + deoxycytidylate
-
485780
bacteriophage T6r+
tetrahydrofolate + 5-hydroxymethyldeoxycytidylate
-
485780
bacteriophage T6r+
?
2.1.2.8
additional information
biosynthesis of enzyme de novo after infection
485780
bacteriophage T6r+
?
-
-
-
-
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.1.2.8
additional information
-
additional information
320 substrate molecules per min per enzyme molecule
bacteriophage T6r+