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Literature summary extracted from

  • Vergis, J.M.; Bulock, K.G.; Fleming, K.G.; Beardsley, G.P.
    Human 5-aminoimidazole-4-carboxamide ribonucleotide transformylase/inosine 5'-monophosphate cyclohydrolase. A bifunctional protein requiring dimerization for transformylase activity but not for cyclohydrolase activity (2001), J. Biol. Chem., 276, 7727-7733.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.1.2.3 medicine
-
 Gallus sp.
2.1.2.3 medicine target for development of inhibitors with potential use as chemotherapeutic agents Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.2.3 cloned from a hepatoma cDNA library Homo sapiens
2.1.2.3 cloned from a placenta cDNA library, overproduced in Escherichia coli Homo sapiens
3.5.4.10
-
 Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.1.2.3
-
 Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.5.4.10 D125A dramatically reduced kcat-value Homo sapiens
3.5.4.10 D125E dramatically reduced kcat-value Homo sapiens
3.5.4.10 D125N dramatically reduced kcat-value Homo sapiens
3.5.4.10 K137A dramatically reduced kcat-value Homo sapiens
3.5.4.10 K137R dramatically reduced kcat-value Homo sapiens
3.5.4.10 K66A dramatically reduced kcat-value Homo sapiens
3.5.4.10 Y104A dramatically reduced kcat-value Homo sapiens
3.5.4.10 Y104A/D125A enzyme activity very low but detectable, contrary to wild type a minimal dependence of specific activity on concentration Homo sapiens
3.5.4.10 Y104F dramatically reduced kcat-value Homo sapiens
3.5.4.10 Y104F/D125A enzyme activity very low but detectable, contrary to wild type a minimal dependence of specific activity on concentration Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.2.3 (6S/R)-5,10-dideaza-5,6,7,8,-tetrahydrofolate
-
 Homo sapiens
2.1.2.3 4-amino-10-methylpteroylglutamic acid methotrexate, non-competitive inhibitor Gallus sp.
2.1.2.3 4-amino-10-methylpteroylglutamic acid methotrexate, non-competitive inhibitor Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.4.10 0.0009
-
 5-Formamido-1-(5-phosphoribosyl)imidazole-4-carboxamide pH 7.5, wild type Homo sapiens
3.5.4.10 0.0027
-
 5-Formamido-1-(5-phosphoribosyl)imidazole-4-carboxamide pH 7.5, mutant D125N Homo sapiens
3.5.4.10 0.0027
-
 5-Formamido-1-(5-phosphoribosyl)imidazole-4-carboxamide pH 7.5, mutant K137R Homo sapiens
3.5.4.10 0.003
-
 5-Formamido-1-(5-phosphoribosyl)imidazole-4-carboxamide pH 7.5, mutant Y104A Homo sapiens
3.5.4.10 0.0036
-
 5-Formamido-1-(5-phosphoribosyl)imidazole-4-carboxamide pH 7.5, mutant Y104F Homo sapiens
3.5.4.10 0.0052
-
 5-Formamido-1-(5-phosphoribosyl)imidazole-4-carboxamide pH 7.5, mutant D125E Homo sapiens
3.5.4.10 0.0091
-
 5-Formamido-1-(5-phosphoribosyl)imidazole-4-carboxamide pH 7.5, mutant D125A Homo sapiens
3.5.4.10 0.031
-
 5-Formamido-1-(5-phosphoribosyl)imidazole-4-carboxamide pH 7.5, mutant K137A Homo sapiens
3.5.4.10 0.115
-
 5-Formamido-1-(5-phosphoribosyl)imidazole-4-carboxamide pH 7.5, mutant K66A Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.2.3 10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazolecarboxamide Saccharomyces cerevisiae
-
 tetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide
-
 r
2.1.2.3 10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazolecarboxamide Homo sapiens penultimate and final steps in the de novo purine biosynthesis pathway tetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide
-
 r
2.1.2.3 10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazolecarboxamide Gallus sp. penultimate and final steps in the de novo purine biosynthesis pathway tetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide
-
 r
2.1.2.3 10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazolecarboxamide Homo sapiens de novo synthesis of purine nucleotides tetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide
-
 r
2.1.2.3 10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazolecarboxamide Gallus sp. de novo synthesis of purine nucleotides tetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.1.2.3 Gallus sp.
-
 avian
-
2.1.2.3 Homo sapiens
-
 human
-
2.1.2.3 Saccharomyces cerevisiae
-
 yeast
-
3.5.4.10 Homo sapiens
-
 bifunctional enzyme 5-aminoimidazole-4-carboxamide ribonucleotide transformylase/inosine 5'-monophosphate cyclohydrolase
-

Reaction

EC Number Reaction Comment Organism Reaction ID
3.5.4.10 IMP + H2O = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide orbital steering mechanism Homo sapiens
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.2.3 Hep-G2 cell hepatocellular carcinoma cell line Homo sapiens
-
2.1.2.3 placenta
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.2.3 10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazolecarboxamide
-
 Saccharomyces cerevisiae tetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide
-
 r
2.1.2.3 10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazolecarboxamide penultimate and final steps in the de novo purine biosynthesis pathway Homo sapiens tetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide
-
 r
2.1.2.3 10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazolecarboxamide penultimate and final steps in the de novo purine biosynthesis pathway Gallus sp. tetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide
-
 r
2.1.2.3 10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazolecarboxamide de novo synthesis of purine nucleotides Homo sapiens tetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide
-
 r
2.1.2.3 10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazolecarboxamide de novo synthesis of purine nucleotides Gallus sp. tetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide
-
 r
2.1.2.3 additional information in all organisms studied to date AICARFT activity is accompanied by inosine monophosphate cyclohydrolase located on the same polypeptide encoded by the purH gene Homo sapiens ?
-
 ?
2.1.2.3 additional information in all organisms studied to date AICARFT activity is accompanied by inosine monophosphate cyclohydrolase located on the same polypeptide encoded by the purH gene Saccharomyces cerevisiae ?
-
 ?
2.1.2.3 additional information in all organisms studied to date AICARFT activity is accompanied by inosine monophosphate cyclohydrolase located on the same polypeptide encoded by the purH gene Gallus sp. ?
-
 ?
2.1.2.3 N10-formyltetrahydrofolic acid + 5-amino-4-imidazole carboxamide ribonucleotide
-
 Homo sapiens tetrahydrofolic acid + 5-formamido-4-imidazole carboxamide ribonucleotide
-
 r
2.1.2.3 N10-formyltetrahydrofolic acid + 5-amino-4-imidazole carboxamide ribonucleotide
-
 Saccharomyces cerevisiae tetrahydrofolic acid + 5-formamido-4-imidazole carboxamide ribonucleotide
-
 r
2.1.2.3 N10-formyltetrahydrofolic acid + 5-amino-4-imidazole carboxamide ribonucleotide
-
 Gallus sp. tetrahydrofolic acid + 5-formamido-4-imidazole carboxamide ribonucleotide
-
 r
3.5.4.10 5-formamido-1-(5-phosphoribosyl)imidazole-4-carboxamide
-
 Homo sapiens IMP + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.2.3 dimer
-
 Homo sapiens
2.1.2.3 dimer
-
 Saccharomyces cerevisiae
2.1.2.3 dimer
-
 Gallus sp.
2.1.2.3 monomer
-
 Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
2.1.2.3 5-aminoimidazole-4-carboxamide ribonucleotide transformylase/inosine 5'-monophosphate cyclohydrolase
-
 Homo sapiens
2.1.2.3 ATIC
-
 Homo sapiens
2.1.2.3 ATIC
-
 Gallus sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.4.10 0.014
-
 5-Formamido-1-(5-phosphoribosyl)imidazole-4-carboxamide pH 7.5, mutant D125A Homo sapiens
3.5.4.10 0.036
-
 5-Formamido-1-(5-phosphoribosyl)imidazole-4-carboxamide pH 7.5, mutant Y104F Homo sapiens
3.5.4.10 0.062
-
 5-Formamido-1-(5-phosphoribosyl)imidazole-4-carboxamide pH 7.5, mutant K66A Homo sapiens
3.5.4.10 0.136
-
 5-Formamido-1-(5-phosphoribosyl)imidazole-4-carboxamide pH 7.5, mutant Y104A Homo sapiens
3.5.4.10 0.147
-
 5-Formamido-1-(5-phosphoribosyl)imidazole-4-carboxamide pH 7.5, mutant D125E Homo sapiens
3.5.4.10 0.276
-
 5-Formamido-1-(5-phosphoribosyl)imidazole-4-carboxamide pH 7.5, mutant K137A Homo sapiens
3.5.4.10 0.507
-
 5-Formamido-1-(5-phosphoribosyl)imidazole-4-carboxamide pH 7.5, mutant K137R Homo sapiens
3.5.4.10 0.603
-
 5-Formamido-1-(5-phosphoribosyl)imidazole-4-carboxamide pH 7.5, mutant D125N Homo sapiens
3.5.4.10 6.08
-
 5-Formamido-1-(5-phosphoribosyl)imidazole-4-carboxamide pH 7.5, mutant D125N Homo sapiens
3.5.4.10 6.08
-
 5-Formamido-1-(5-phosphoribosyl)imidazole-4-carboxamide pH 7.5, mutant K137R Homo sapiens
3.5.4.10 8.6
-
 5-Formamido-1-(5-phosphoribosyl)imidazole-4-carboxamide pH 7.5, wild type Homo sapiens