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Literature summary extracted from

  • Caperelli, C.A.; Giroux, E.L.
    The human glycinamide ribonucleotide transformylase domain: purification, characterization, and kinetic mechanism (1997), Arch. Biochem. Biophys., 341, 98-103.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.1.2.2 medicine pharmacological importance, target enzyme for chemotherapy, development of novel antifolate drugs, to be used as anti-cancer drugs Escherichia coli
2.1.2.2 medicine pharmacological importance, target enzyme for chemotherapy, development of novel antifolate drugs, to be used as anti-cancer drugs Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.2.2 cloned and expressed in Escherichia coli BL21(DE3)pLysS Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.2.2 (alpha,beta)-hydroxyacetamide ribonucleotide
-
 Homo sapiens
2.1.2.2 10-Acetyl-5,8-dideazafolate
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.2.2 0.0011
-
 beta-glycinamide ribonucleotide
-
 Homo sapiens
2.1.2.2 0.0015
-
 10-formyl-5,8-dideazafolate recombinant enzyme, expressed in E.coli Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.2.2 21800
-
 recombinant enzyme, predicted from cDNA Homo sapiens
2.1.2.2 21840
-
 recombinant enzyme, MALDI-TOF mass spectral analysis Homo sapiens
2.1.2.2 25700
-
 SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.2.2 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide Bacillus subtilis
-
 tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
-
 ?
2.1.2.2 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide Saccharomyces cerevisiae
-
 tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
-
 ?
2.1.2.2 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide Gallus gallus essential enzyme in DNA synthesis, third enzyme in de novo purine biosynthesis pathway tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
-
 ?
2.1.2.2 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide Escherichia coli essential enzyme in DNA synthesis, third enzyme in de novo purine biosynthesis pathway tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
-
 ?
2.1.2.2 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide Homo sapiens essential enzyme in DNA synthesis, third enzyme in de novo purine biosynthesis pathway tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.2.2 Bacillus subtilis
-
-
-
2.1.2.2 Escherichia coli
-
-
-
2.1.2.2 Gallus gallus
-
 chicken
-
2.1.2.2 Homo sapiens
-
 human
-
2.1.2.2 Saccharomyces cerevisiae
-
 yeast
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.2.2 recombinant enzyme, expressed in E. coli Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.2.2 liver
-
 Gallus gallus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.2.2 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
-
 Gallus gallus tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
-
 ?
2.1.2.2 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
-
 Bacillus subtilis tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
-
 ?
2.1.2.2 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
-
 Escherichia coli tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
-
 ?
2.1.2.2 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
-
 Homo sapiens tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
-
 ?
2.1.2.2 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
-
 Saccharomyces cerevisiae tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
-
 ?
2.1.2.2 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide essential enzyme in DNA synthesis, third enzyme in de novo purine biosynthesis pathway Gallus gallus tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
-
 ?
2.1.2.2 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide essential enzyme in DNA synthesis, third enzyme in de novo purine biosynthesis pathway Escherichia coli tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
-
 ?
2.1.2.2 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide essential enzyme in DNA synthesis, third enzyme in de novo purine biosynthesis pathway Homo sapiens tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
-
 ?
2.1.2.2 additional information monofunctional enzyme Bacillus subtilis ?
-
 ?
2.1.2.2 additional information monofunctional enzyme Escherichia coli ?
-
 ?
2.1.2.2 additional information trifunctional enzyme, GAR synthetase, aminoimidazole ribonucleotide synthetase and GAR transformylase Gallus gallus ?
-
 ?
2.1.2.2 additional information trifunctional enzyme, GAR synthetase, aminoimidazole ribonucleotide synthetase and GAR transformylase Homo sapiens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.2.2 monomer
-
 Gallus gallus
2.1.2.2 monomer
-
 Bacillus subtilis
2.1.2.2 monomer
-
 Escherichia coli
2.1.2.2 monomer
-
 Saccharomyces cerevisiae
2.1.2.2 monomer 1 * 25700, SDS-PAGE Homo sapiens