Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Nixon, A.E.; Benkovic, S.J.
    Improvement in the efficiency of formyl transfer of a GAR transformylase hybrid enzyme (2000), Protein Eng., 13, 323-327.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.2.2 hybrid glycinamide ribonucleotide transformylase, ribonucleotide binding domain from purN, catalytic machinery and formyl tetrahydrofolate binding domain from purU encoded formyl tetrahydrofolate hydrolase Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.2.2 0.016
-
 beta-glycinamide ribonucleotide purN/purU hybrid enzyme Escherichia coli
2.1.2.2 0.017
-
 10-formyl-5,8-dideazafolate
-
 Escherichia coli
2.1.2.2 0.019
-
 beta-glycinamide ribonucleotide
-
 Escherichia coli
2.1.2.2 0.035
-
 10-formyl-5,8-dideazafolate purN/purU hybrid enzyme Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.2.2 23000
-
 purN encoded, DNA sequencing Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.1.2.2 Escherichia coli
-
 strains DH5alpha, BL21(DE3)
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.2.2 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
-
 Escherichia coli tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
-
 ?