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Literature summary extracted from

  • Marolewski, A.; Smith, J.M.; Benkovic, S.J.
    Cloning and characterization of a new purine biosynthetic enzyme: a non-folate glycinamide ribonucleotide transformylase from E. coli (1994), Biochemistry, 33, 2531-2537.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.2.2 second GAR transformylase encoded by the Escherichia coli purT gene Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.2.2 0.00368
-
 acetate purT gene product Escherichia coli
2.1.2.2 0.0101
-
 beta-glycinamide ribonucleotide purT gene product Escherichia coli
2.1.2.2 0.012
-
 beta-glycinamide ribonucleotide pH 7.5, tetrahydrofolate as cofactor Escherichia coli
2.1.2.2 0.0774
-
 ATP purT gene product Escherichia coli
2.1.2.2 0.319
-
 formate purT gene product Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.2.2 Mg2+ purT gene product Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.2.2 41900
-
 product of the purT gene, SDS-PAGE Escherichia coli
2.1.2.2 42000
-
 product of the purT gene, deduced from predicted amino acid sequence Escherichia coli
2.1.2.2 42400
-
 product of the purT gene Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.1.2.2 Escherichia coli
-
-
-
2.1.2.2 Escherichia coli TX635
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.2.2 transformylase, product of the purT gene Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.2.2 additional information
-
 purT gene product, 0.052 mg /min.mg.mL Escherichia coli
2.1.2.2 14
-
 purN gene product Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.2.2 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
-
 Escherichia coli tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
-
 ?
2.1.2.2 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
-
 Escherichia coli TX635 tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
-
 ?
2.1.2.2 acetate + ATP novel non-folate glycinamide ribonucleotide transformylase purT gene product, no significant homology to known EC 2.1.2.2 but significant homology to the purK protein also involved in purine biosynthesis Escherichia coli acetyl phosphate + ADP
-
 ?
2.1.2.2 acetate + ATP novel non-folate glycinamide ribonucleotide transformylase purT gene product, no significant homology to known EC 2.1.2.2 but significant homology to the purK protein also involved in purine biosynthesis Escherichia coli TX635 acetyl phosphate + ADP
-
 ?
2.1.2.2 formate + ATP + beta-glycinamide ribonucleotide novel non-folate glycinamide ribonucleotide transformylase purT gene product, no significant homology to known EC2.1.2.2 but significant homology to the purK protein also involved in purine biosynthesis Escherichia coli beta-formylglycinamide ribonucleotide + ADP
-
 ?
2.1.2.2 formate + ATP + beta-glycinamide ribonucleotide novel non-folate glycinamide ribonucleotide transformylase purT gene product, no significant homology to known EC2.1.2.2 but significant homology to the purK protein also involved in purine biosynthesis Escherichia coli TX635 beta-formylglycinamide ribonucleotide + ADP
-
 ?
2.1.2.2 additional information 10-formyl-dideazaformate, 5-formyltetrahydroformate, formylaminoimidazolecarboxamide ribonucleotide, formylmethionine, formiminoglutamate, GTP, CTP, TTP, ITP and ZTP are no substrates for the purT GAR transformylase Escherichia coli ?
-
 ?
2.1.2.2 additional information purT GAR transformylase is the first unknown de novo purine biosynthetic enzyme to be discovered in the last 30 years and represents another step forward in understanding cellular control of purine levels Escherichia coli ?
-
 ?
2.1.2.2 additional information 10-formyl-dideazaformate, 5-formyltetrahydroformate, formylaminoimidazolecarboxamide ribonucleotide, formylmethionine, formiminoglutamate, GTP, CTP, TTP, ITP and ZTP are no substrates for the purT GAR transformylase Escherichia coli TX635 ?
-
 ?
2.1.2.2 additional information purT GAR transformylase is the first unknown de novo purine biosynthetic enzyme to be discovered in the last 30 years and represents another step forward in understanding cellular control of purine levels Escherichia coli TX635 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.2.2 monomer 1 * 42000, product of the purT gene Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.2.2 8
-
 purTgene product Escherichia coli