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Literature summary extracted from

  • Sanghani, S.P.; Moran, R.G.
    Tight binding of folate substrates and inhibitors to recombinant mouse glycinamide ribonucleotide formyltransferase (1997), Biochemistry, 36, 10506-10516.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.1.2.2 medicine
-
 Escherichia coli
2.1.2.2 medicine
-
 Homo sapiens
2.1.2.2 medicine inhibitor 5,10-dideazatetrahydrofolate is antiproliferate and cytotoxic against mammalian tumor cells in culture and have broad spectrum activity against transplantable mouse tumors and human tumor xenografts Mus musculus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.2.2 cDNA, recombinant enzyme expressed in insect cells with a recombinant baculovirus Mus musculus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.1.2.2
-
 Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.2.2 10-formyl-5,8-dideazafolic acid hexaglutamate
-
 Mus musculus
2.1.2.2 2',5'-thiophene dideazatetrahydrofolate LY309887, diastereomer B Homo sapiens
2.1.2.2 2',5'-thiophene dideazatetrahydrofolate LY309887, diastereomer B Mus musculus
2.1.2.2 2-deaminodideazatetrahydrofolate
-
 Mus musculus
2.1.2.2 5,10-dideazatetrahydrofolate
-
 Escherichia coli
2.1.2.2 5,10-dideazatetrahydrofolate
-
 Homo sapiens
2.1.2.2 5,10-dideazatetrahydrofolate
-
 Mus musculus
2.1.2.2 5,8-Dideazafolate
-
 Escherichia coli
2.1.2.2 5,8-dideazafolate hexaglutamate
-
 Mus musculus
2.1.2.2 5,8-dideazapteroyl hexaglutamate
-
 Mus musculus
2.1.2.2 5-deazatetrahydrofolate
-
 Mus musculus
2.1.2.2 dideazatetrahydrofolate
-
 Mus musculus
2.1.2.2 dideazatetrahydrofolate hexaglutamate
-
 Mus musculus
2.1.2.2 homo-dideazatetrahydrofolate
-
 Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.2.2 0.000075
-
 10-formyl-5,8-dideazafolate recombinant enzyme Mus musculus
2.1.2.2 0.0013
-
 10-formyl-5,8-dideazafolate
-
 Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.2.2 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide Gallus gallus essential enzyme in DNA synthesis, third enzyme in de novo purine biosynthesis pathway tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
-
 ?
2.1.2.2 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide Mus musculus essential enzyme in DNA synthesis, third enzyme in de novo purine biosynthesis pathway tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
-
 ?
2.1.2.2 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide Escherichia coli essential enzyme in DNA synthesis, third enzyme in de novo purine biosynthesis pathway tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
-
 ?
2.1.2.2 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide Homo sapiens essential enzyme in DNA synthesis, third enzyme in de novo purine biosynthesis pathway tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
-
 ?
2.1.2.2 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide Mus musculus carries out the second, third and fifth sequential reactions of de novo purine synthesis pathway tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.2.2 Escherichia coli
-
-
-
2.1.2.2 Gallus gallus
-
 chicken
-
2.1.2.2 Homo sapiens
-
 human
-
2.1.2.2 Mus musculus
-
 mouse
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.2.2
-
 Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.2.2 L-1210 cell murine leukemia Mus musculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.2.2 7
-
-
 Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.2.2 10-formyl-5,8-dideazafolate + carbocyclic beta-glycinamide ribonucleotide
-
 Mus musculus 5,8-dideazafolate + N-formyl carbocyclic beta-glycinamide ribonucleotide
-
 ?
2.1.2.2 10-formyl-5,8-dideazafolate + carbocyclic beta-glycinamide ribonucleotide
-
 Escherichia coli 5,8-dideazafolate + N-formyl carbocyclic beta-glycinamide ribonucleotide
-
 ?
2.1.2.2 10-formyl-5,8-dideazafolate + carbocyclic beta-glycinamide ribonucleotide
-
 Homo sapiens 5,8-dideazafolate + N-formyl carbocyclic beta-glycinamide ribonucleotide
-
 ?
2.1.2.2 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
-
 Gallus gallus tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
-
 ?
2.1.2.2 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
-
 Mus musculus tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
-
 ?
2.1.2.2 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
-
 Escherichia coli tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
-
 ?
2.1.2.2 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
-
 Homo sapiens tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
-
 ?
2.1.2.2 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide essential enzyme in DNA synthesis, third enzyme in de novo purine biosynthesis pathway Gallus gallus tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
-
 ?
2.1.2.2 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide essential enzyme in DNA synthesis, third enzyme in de novo purine biosynthesis pathway Mus musculus tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
-
 ?
2.1.2.2 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide essential enzyme in DNA synthesis, third enzyme in de novo purine biosynthesis pathway Escherichia coli tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
-
 ?
2.1.2.2 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide essential enzyme in DNA synthesis, third enzyme in de novo purine biosynthesis pathway Homo sapiens tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
-
 ?
2.1.2.2 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide carries out the second, third and fifth sequential reactions of de novo purine synthesis pathway Mus musculus tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
-
 ?
2.1.2.2 additional information
-
 Escherichia coli ?
-
 ?
2.1.2.2 additional information trifunctional enzyme, GAR synthetase, aminoimidazole ribonucleotide synthetase and GAR transformylase Gallus gallus ?
-
 ?
2.1.2.2 additional information trifunctional enzyme, GAR synthetase, aminoimidazole ribonucleotide synthetase and GAR transformylase Mus musculus ?
-
 ?
2.1.2.2 additional information trifunctional enzyme, GAR synthetase, aminoimidazole ribonucleotide synthetase and GAR transformylase Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.2.2 GARFT
-
 Gallus gallus
2.1.2.2 GARFT
-
 Mus musculus
2.1.2.2 GARFT
-
 Escherichia coli
2.1.2.2 GARFT
-
 Homo sapiens