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Literature summary extracted from

  • Smith, G.K.; Mueller, W.T.; Wasserman, G.F.; Taylor, W.D.; Benkovic, S.J.
    Characterization of the enzyme complex involving the folate-requiring enzymes of de novo purine biosynthesis (1980), Biochemistry, 19, 4313-4321.
    View publication on PubMed

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.2.2 110000 120000 gel filtration, sucrose density gradient ultracentrifugation, sedimentation velocity Gallus gallus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.2.2 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide Gallus gallus essential enzyme in DNA synthesis, third enzyme in de novo purine biosynthesis pathway tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
-
 ?
2.1.2.2 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide Escherichia coli essential enzyme in DNA synthesis, third enzyme in de novo purine biosynthesis pathway tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
-
 ?
3.5.4.9 5,10-methenyltetrahydrofolate + H2O Gallus gallus
-
 10-formyltetrahydrofolate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.2.2 Escherichia coli
-
-
-
2.1.2.2 Gallus gallus
-
 chicken
-
3.5.4.9 Gallus gallus
-
 chicken, trifunctional enzyme
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.2.2 copurification of trifunctional 5,10-methenyl-, 5,10-methylene, and 10-formylH4folate synthetase, aminoimidazole carboxamide ribotide transformylase and serine transhydroxymethylase Gallus gallus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.4.9 30.6
-
-
 Gallus gallus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.2.2 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
-
 Gallus gallus tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
-
 ?
2.1.2.2 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
-
 Escherichia coli tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
-
 ?
2.1.2.2 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide essential enzyme in DNA synthesis, third enzyme in de novo purine biosynthesis pathway Gallus gallus tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
-
 ?
2.1.2.2 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide essential enzyme in DNA synthesis, third enzyme in de novo purine biosynthesis pathway Escherichia coli tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
-
 ?
2.1.2.2 additional information trifunctional enzyme, GAR synthetase, aminoimidazole ribonucleotide synthetase and GAR transformylase Gallus gallus ?
-
 ?
3.5.4.9 5,10-methenyltetrahydrofolate + H2O
-
 Gallus gallus 10-formyltetrahydrofolate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.2.2 dimer
-
 Escherichia coli
2.1.2.2 dimer 2 * 55000-57000, SDS-PAGE Gallus gallus