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Literature summary extracted from

  • Inglese, J.; Johnson, D.L.; Shiau, A.; Smith, J.M.; Benkovic, S.J.
    Subcloning, characterization, and affinity labeling of Escherichia coli glycinamide ribonucleotide transformylase (1990), Biochemistry, 29, 1436-1443.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.2.2 harboring expression vector pJS167, encoding the purN gene Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.2.2 (6S)-N10-formyltetrahydrofolate
-
 Escherichia coli
2.1.2.2 beta-thioglycinamide dideazafolate
-
 Escherichia coli
2.1.2.2 dideazafolate
-
 Escherichia coli
2.1.2.2 N10-(Bromoacetyl)-5,8-dideazafolate
-
 Escherichia coli
2.1.2.2 N10-formyl-2,4-diaminodideazafolate
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.2.2 0.008
-
 beta-glycinamide ribonucleotide pH 8.5, tetrahydrofolate as cofactor Escherichia coli
2.1.2.2 0.0122
-
 beta-glycinamide ribonucleotide pH 7.5, tetrahydrofolate as cofactor Escherichia coli
2.1.2.2 0.0167
-
 N10-formyldideazafolate pH 7.5 Escherichia coli
2.1.2.2 0.0192
-
 beta-glycinamide ribonucleotide pH 7.5, dideazafolate as cofactor Escherichia coli
2.1.2.2 0.0235
-
 beta-glycinamide ribonucleotide pH 8.5, dideazafolate as cofactor Escherichia coli
2.1.2.2 0.0365
-
 N10-formyldideazafolate pH 8.5 Escherichia coli
2.1.2.2 0.0465
-
 carbocyclic beta-glycinamide ribonucleotide
-
 Escherichia coli
2.1.2.2 0.0775
-
 (6R)-N10-formyltetrahydrofolate pH 8.5 Escherichia coli
2.1.2.2 0.0848
-
 (6R)-N10-formyltetrahydrofolate pH 7.5 Escherichia coli
2.1.2.2 0.15
-
 N10-Formyl-8-deazafolate
-
 Escherichia coli
2.1.2.2 0.19
-
 N10-formyl-5-deazafolate
-
 Escherichia coli
2.1.2.2 0.6
-
 N10-formylfolate
-
 Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.2.2 21060
-
 calculated from peptide sequence deduced from the cDNA Bacillus subtilis
2.1.2.2 23240
-
 calculated from peptide sequence deduced from the cDNA Escherichia coli
2.1.2.2 24000
-
 equilibrium sedimentation ultracentrifugation Escherichia coli
2.1.2.2 145000
-
-
 Drosophila melanogaster

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.2.2 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide Cricetulus griseus
-
 tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
-
 ?
2.1.2.2 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide Bacillus subtilis
-
 tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
-
 ?
2.1.2.2 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide Saccharomyces cerevisiae
-
 tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
-
 ?
2.1.2.2 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide Gallus gallus essential enzyme in DNA synthesis, third enzyme in de novo purine biosynthesis pathway tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
-
 ?
2.1.2.2 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide Drosophila melanogaster essential enzyme in DNA synthesis, third enzyme in de novo purine biosynthesis pathway tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
-
 ?
2.1.2.2 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide Mus musculus essential enzyme in DNA synthesis, third enzyme in de novo purine biosynthesis pathway tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
-
 ?
2.1.2.2 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide Escherichia coli essential enzyme in DNA synthesis, third enzyme in de novo purine biosynthesis pathway tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
-
 ?
2.1.2.2 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide Homo sapiens essential enzyme in DNA synthesis, third enzyme in de novo purine biosynthesis pathway tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.2.2 Bacillus subtilis
-
-
-
2.1.2.2 Cricetulus griseus
-
 chinese hamster
-
2.1.2.2 Drosophila melanogaster
-
 fruit fly
-
2.1.2.2 Escherichia coli
-
-
-
2.1.2.2 Gallus gallus
-
 chicken
-
2.1.2.2 Homo sapiens
-
 human
-
2.1.2.2 Mus musculus
-
 mouse
-
2.1.2.2 Saccharomyces cerevisiae
-
 yeast
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.2.2
-
 Escherichia coli

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.2.2 HeLa cell O cell Homo sapiens
-
2.1.2.2 L-1210 cell murine leukemia Mus musculus
-
2.1.2.2 L-5178-Y cell murine lymphoma cell line Mus musculus
-
2.1.2.2 ovary
-
 Cricetulus griseus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.2.2 11.6
-
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.2.2 (6R)-N10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
-
 Gallus gallus (6R)-tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
-
 ?
2.1.2.2 (6R)-N10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
-
 Escherichia coli (6R)-tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
-
 ?
2.1.2.2 10-formyl-5,8-dideazafolate + carbocyclic beta-glycinamide ribonucleotide
-
 Mus musculus 5,8-dideazafolate + N-formyl carbocyclic beta-glycinamide ribonucleotide
-
 ?
2.1.2.2 10-formyl-5,8-dideazafolate + carbocyclic beta-glycinamide ribonucleotide
-
 Escherichia coli 5,8-dideazafolate + N-formyl carbocyclic beta-glycinamide ribonucleotide
-
 ?
2.1.2.2 10-formyl-5,8-dideazafolate + carbocyclic beta-glycinamide ribonucleotide
-
 Homo sapiens 5,8-dideazafolate + N-formyl carbocyclic beta-glycinamide ribonucleotide
-
 ?
2.1.2.2 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
-
 Gallus gallus tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
-
 ?
2.1.2.2 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
-
 Cricetulus griseus tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
-
 ?
2.1.2.2 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
-
 Drosophila melanogaster tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
-
 ?
2.1.2.2 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
-
 Bacillus subtilis tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
-
 ?
2.1.2.2 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
-
 Mus musculus tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
-
 ?
2.1.2.2 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
-
 Escherichia coli tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
-
 ?
2.1.2.2 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
-
 Homo sapiens tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
-
 ?
2.1.2.2 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
-
 Saccharomyces cerevisiae tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
-
 ?
2.1.2.2 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide essential enzyme in DNA synthesis, third enzyme in de novo purine biosynthesis pathway Gallus gallus tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
-
 ?
2.1.2.2 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide essential enzyme in DNA synthesis, third enzyme in de novo purine biosynthesis pathway Drosophila melanogaster tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
-
 ?
2.1.2.2 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide essential enzyme in DNA synthesis, third enzyme in de novo purine biosynthesis pathway Mus musculus tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
-
 ?
2.1.2.2 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide essential enzyme in DNA synthesis, third enzyme in de novo purine biosynthesis pathway Escherichia coli tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
-
 ?
2.1.2.2 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide essential enzyme in DNA synthesis, third enzyme in de novo purine biosynthesis pathway Homo sapiens tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
-
 ?
2.1.2.2 10-formyltetrahydrofolate + beta-5-phospho-D-ribosylglycinamide
-
 Escherichia coli tetrahydrofolate + beta-5'-phosphoribosyl-N-formylglycinamide
-
 ?
2.1.2.2 additional information
-
 Bacillus subtilis ?
-
 ?
2.1.2.2 additional information
-
 Escherichia coli ?
-
 ?
2.1.2.2 additional information 3 activities in a multifunctional protein, posessing glycinamide ribonucleotide synthetase, glycinamide ribonucleotidetransformylase and aminoimidazole ribonucleotide synthetase Gallus gallus ?
-
 ?
2.1.2.2 additional information 3 activities in a multifunctional protein, posessing glycinamide ribonucleotide synthetase, glycinamide ribonucleotidetransformylase and aminoimidazole ribonucleotide synthetase Mus musculus ?
-
 ?
2.1.2.2 additional information 3 activities in a multifunctional protein, posessing glycinamide ribonucleotide synthetase, glycinamide ribonucleotidetransformylase and aminoimidazole ribonucleotide synthetase Homo sapiens ?
-
 ?
2.1.2.2 N10-formyl-5-deazafolate + glycinamide ribonucleotide
-
 Escherichia coli formylglycinamide ribonucleotide + 5-deazafolate
-
 ?
2.1.2.2 N10-formyl-8-deazafolate + glycinamide ribonucleotide
-
 Escherichia coli formylglycinamide ribonucleotide + 8-deazafolate
-
 ?
2.1.2.2 N10-formyldideazafolate + 5-phospho-D-ribosylglycinamide
-
 Escherichia coli dideazafolate + 5'-phosphoribosyl-N-formylglycinamide
-
 ?
2.1.2.2 N10-formylfolate + glycinamide ribonucleotide
-
 Escherichia coli folate + formylglycinamide ribonucleotide
-
 ?
2.1.2.2 N5,N10-anhydroformyltetrahydrofolate + glycinamide ribotide + H2O
-
 Escherichia coli N10-formyltetrahydrofolate + formylglycinamide ribotide
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.2.2 monomer
-
 Gallus gallus
2.1.2.2 monomer
-
 Bacillus subtilis
2.1.2.2 monomer
-
 Mus musculus
2.1.2.2 monomer
-
 Homo sapiens
2.1.2.2 monomer
-
 Saccharomyces cerevisiae
2.1.2.2 monomer 1 + 25000, SDS-PAGE Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.2.2 0.016
-
 N10-formylfolate
-
 Escherichia coli
2.1.2.2 0.069
-
 N10-Formyl-8-deazafolate
-
 Escherichia coli
2.1.2.2 1.52
-
 N10-formyl-5-deazafolate
-
 Escherichia coli
2.1.2.2 13.5
-
 (6R)-N10-formyltetrahydrofolate pH 7.5 Escherichia coli
2.1.2.2 16.1
-
 N10-formyl-5,8-dideazafolate pH 7.5 Escherichia coli
2.1.2.2 20.7
-
 (6R)-N10-formyltetrahydrofolate pH 8.5 Escherichia coli
2.1.2.2 33
-
 carbocyclic glycinamide ribonucleotide
-
 Escherichia coli
2.1.2.2 40
-
 N10-formyl-5,8-dideazafolate pH 8.5 Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.2.2 8.5
-
-
 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.2.2 L-(-)-10-formyltetrahydrofolate
-
 Gallus gallus
2.1.2.2 L-(-)-10-formyltetrahydrofolate
-
 Escherichia coli