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Literature summary extracted from

  • Harper, D.B.; Kennedy, J.T.
    Purification and properties of S-adenosylmethionine: aldoxime O-methyltransferase from Pseudomonas sp. N.C.I.B. 11652 (1985), Biochem. J., 226, 147-153.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.1.1.91 additional information requires thiol-protecting agents for activity Pseudomonas sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.91 Ag2+ 100% inhibition at 1 mM Pseudomonas sp.
2.1.1.91 Hg2+ 100% inhibition at 0.05 mM Pseudomonas sp.
2.1.1.91 iodoacetamide 55% inhibition at 0.5 mM Pseudomonas sp.
2.1.1.91 additional information EDTA and Mg2+ are not inhibitory Pseudomonas sp.
2.1.1.91 N-ethylmaleimide 56% inhibition at 0.05 mM Pseudomonas sp.
2.1.1.91 Phenylmercuriacetate 95% inhibition at 0.025 mM Pseudomonas sp.
2.1.1.91 S-adenosyl-L-homocysteine competitive inhibition Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.91 0.14
-
 2-Methylbutyraldoxime
-
 Pseudomonas sp.
2.1.1.91 0.15
-
 S-adenosyl-L-methionine
-
 Pseudomonas sp.
2.1.1.91 0.16
-
 3-Methylbutyraldoxime
-
 Pseudomonas sp.
2.1.1.91 0.24 0.27 Isobutyraldoxime
-
 Pseudomonas sp.
2.1.1.91 0.67
-
 n-Pentanaldoxime
-
 Pseudomonas sp.
2.1.1.91 0.98
-
 n-Butyraldoxime
-
 Pseudomonas sp.
2.1.1.91 1.11
-
 Methacrylaldoxime
-
 Pseudomonas sp.
2.1.1.91 1.54
-
 n-Propionaldoxime
-
 Pseudomonas sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.1.91 additional information Mg2+ has no significant effect Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.91 37500
-
 2 * 37500, SDS-PAGE Pseudomonas sp.
2.1.1.91 84000
-
 gel filtration Pseudomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.91 Pseudomonas sp.
-
 N.C.I.B. 11652
-
2.1.1.91 Pseudomonas sp. NCIB 11652
-
 N.C.I.B. 11652
-

Oxidation Stability

EC Number Oxidation Stability Organism
2.1.1.91 labile to O2, thiol-protecting agents are necessary Pseudomonas sp.

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.91 220fold Pseudomonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.91 247.4
-
-
 Pseudomonas sp.

Storage Stability

EC Number Storage Stability Organism
2.1.1.91 -15°C, 100 mM potassium phosphate buffer, pH 7.5, 5 mM DTT, several months Pseudomonas sp.
2.1.1.91 0°C, (NH4)2SO4-paste has a half life of 10 weeks Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.91 2-methylbutyraldoxime + S-adenosyl-L-methionine highest activity Pseudomonas sp. 2-methylbutyraldoxime methyl ether + S-adenosyl-L-homocysteine
-
 ?
2.1.1.91 2-methylbutyraldoxime + S-adenosyl-L-methionine highest activity Pseudomonas sp. NCIB 11652 2-methylbutyraldoxime methyl ether + S-adenosyl-L-homocysteine
-
 ?
2.1.1.91 3-methylbutyraldoxime + S-adenosyl-L-methionine
-
 Pseudomonas sp. 3-methylbutyraldoxime methyl ether + S-adenosyl-L-homocysteine
-
 ?
2.1.1.91 3-methylbutyraldoxime + S-adenosyl-L-methionine
-
 Pseudomonas sp. NCIB 11652 3-methylbutyraldoxime methyl ether + S-adenosyl-L-homocysteine
-
 ?
2.1.1.91 isobutyraldoxime + S-adenosyl-L-methionine
-
 Pseudomonas sp. isobutyraldoxime methyl ether + S-adenosyl-L-homocysteine
-
 ?
2.1.1.91 isobutyraldoxime + S-adenosyl-L-methionine
-
 Pseudomonas sp. NCIB 11652 isobutyraldoxime methyl ether + S-adenosyl-L-homocysteine
-
 ?
2.1.1.91 methacrylaldoxime + S-adenosyl-L-methionine
-
 Pseudomonas sp. methacrylaldoxime methyl ether + S-adenosyl-L-homocysteine
-
 ?
2.1.1.91 additional information no reaction with n-hexanaldoxime, benzaldoxime, 2-butoxime Pseudomonas sp. ?
-
 ?
2.1.1.91 additional information no reaction with n-hexanaldoxime, benzaldoxime, 2-butoxime Pseudomonas sp. NCIB 11652 ?
-
 ?
2.1.1.91 n-butyraldoxime + S-adenosyl-L-methionine
-
 Pseudomonas sp. n-butyraldoxime methyl ether + S-adenosyl-L-homocysteine
-
 ?
2.1.1.91 n-pentanaldoxime + S-adenosyl-L-methionine
-
 Pseudomonas sp. n-pentanaldoxime methyl ether + S-adenosyl-L-homocysteine
-
 ?
2.1.1.91 n-propionaldoxime + S-adenosyl-L-methionine
-
 Pseudomonas sp. n-propionaldoxime methyl ether + S-adenosyl-L-homocysteine
-
 ?
2.1.1.91 n-propionaldoxime + S-adenosyl-L-methionine
-
 Pseudomonas sp. NCIB 11652 n-propionaldoxime methyl ether + S-adenosyl-L-homocysteine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.91 dimer 2 * 37500, SDS-PAGE Pseudomonas sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.91 25
-
 assay at Pseudomonas sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.1.1.91 additional information
-
 temperature sensitive: 0-3°C are necessary during purification Pseudomonas sp.
2.1.1.91 35
-
 rapid inactivation above Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.91 10.25
-
-
 Pseudomonas sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.1.1.91 9 10.4 less than 50% of maximal activity below pH 9.0, almost no activity above pH 10.4 Pseudomonas sp.

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.1.1.91 0.027
-
 S-adenosyl-L-homocysteine
-
 Pseudomonas sp.