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Literature summary extracted from

  • Van der Meijden, P.; te Brömmelstroet, B.W.; Poirot, C.M.; van der Drift, C.; Vogels, G.D.
    Purification and properties of methanol:5-hydroxybenzimidazolylcobamide methyltransferase from Methanosarcina barkeri (1984), J. Bacteriol., 160, 629-635.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.1.1.90 ATP half-maximal activation at 0.16 mM ATP Methanosarcina barkeri
2.1.1.90 additional information reductive activation by a mixture of H2, ferredoxin needed in the reductive activation process Methanosarcina barkeri
2.1.1.90 additional information reductive activation by a mixture of H2, ferredoxin and hydrogenase but also by CO, ATP, GTP or CTP needed in the reductive activation process Methanosarcina barkeri

General Stability

EC Number General Stability Organism
2.1.1.90 presence of divalent cations e.g. Mg2+, Mn2+, Sr2+, Ca2+ or Ba2+ are required for stability Methanosarcina barkeri

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.90 HgCl2 1 mM, 90% inhibition Methanosarcina barkeri
2.1.1.90 Na2SO3 1 mM, 50% inhibition Methanosarcina barkeri
2.1.1.90 NaNO2 0.1 mM, 90% inhibition Methanosarcina barkeri
2.1.1.90 NH4Cl 400 mM, 93% inhibition Methanosarcina barkeri
2.1.1.90 O2 inactivation by O2 and other oxidizing agents Methanosarcina barkeri
2.1.1.90 pyridoxal 5'-phosphate 1 mM, 74% inhibition Methanosarcina barkeri

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.1.90 Ba2+ metal-free enzyme preparation has no activity, addition of Ba2+ restores 27% of the original activity Methanosarcina barkeri
2.1.1.90 Ca2+ metal-free enzyme preparation has no activity, addition of Ca2+ restores 35% of the original activity Methanosarcina barkeri
2.1.1.90 Co2+ metal-free enzyme preparation has no activity, addition of Co+ restores 46% of the original activity Methanosarcina barkeri
2.1.1.90 Mg2+ metal-free enzyme preparation has no activity, addition of Mg2+ restores 66% of the original activity Methanosarcina barkeri
2.1.1.90 Mn2+ metal-free enzyme preparation has no activity, addition of Mn2+ restores 27% of the original activity Methanosarcina barkeri
2.1.1.90 Ni2+ metal-free enzyme preparation has no activity, addition of Ni2+ restores 21% of the original activity Methanosarcina barkeri
2.1.1.90 Sr2+ metal-free enzyme preparation has no activity, addition of Sr2+ restores 20% of the original activity Methanosarcina barkeri

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.90 34000
-
alpha2,beta, 2 * 34000 + 1 * 53000, SDS-PAGE in presence of 2-mercaptoethanol Methanosarcina barkeri
2.1.1.90 53000
-
alpha2,beta, 2 * 34000 + 1 * 53000, SDS-PAGE in presence of 2-mercaptoethanol Methanosarcina barkeri
2.1.1.90 122000
-
PAGE with different gel concentrations Methanosarcina barkeri

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.90 methanol + 5-hydroxybenzimidazolylcobamide Methanosarcina barkeri one of the enzymes responsible for the transmethylation from methanol to coenzyme M Co-methyl-Co-5-hydroxybenzimidazolylcobamide + H2O
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.90 Methanosarcina barkeri Q46EH3
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
2.1.1.90 oxygen-sensitive Methanosarcina barkeri

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.90
-
Methanosarcina barkeri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.90 methanol + 5-hydroxybenzimidazolylcobamide
-
Methanosarcina barkeri Co-methyl-Co-5-hydroxybenzimidazolylcobamide + H2O
-
?
2.1.1.90 methanol + 5-hydroxybenzimidazolylcobamide one of the enzymes responsible for the transmethylation from methanol to coenzyme M Methanosarcina barkeri Co-methyl-Co-5-hydroxybenzimidazolylcobamide + H2O
-
?

Subunits

EC Number Subunits Comment Organism
2.1.1.90 trimer alpha2,beta, 2 * 34000 + 1 * 53000, SDS-PAGE in presence of 2-mercaptoethanol Methanosarcina barkeri

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.90 37
-
assay at Methanosarcina barkeri

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.90 7.2
-
assay at Methanosarcina barkeri

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.90 5-hydroxybenzimidazolylcobamide 3-4 molecules bound, acting as methyl acceptor Methanosarcina barkeri