BRENDA - Enzyme Database

Cyclopropane fatty acid synthase of Escherichia coli. Stabilization, purification, and interaction with phospholipid vesicles

Taylor, F.R.; Cronan, J.E.; Biochemistry 18, 3292-3300 (1979)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.1.1.79
5,5'-dithiobis(2-nitrobenzoic acid)
reversed by addition of dithiothreitol
Escherichia coli
2.1.1.79
NEM
-
Escherichia coli
2.1.1.79
p-hydroxymercuribenzoate
-
Escherichia coli
2.1.1.79
S-adenosylhomocysteine
-
Escherichia coli
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.1.1.79
0.09
-
S-adenosyl-L-methionine
-
Escherichia coli
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.1.1.79
membrane
loosely associated with the inner membrane
Escherichia coli
16020
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.1.1.79
90000
-
equilibrium sedimentation, gel filtration
Escherichia coli
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.1.1.79
S-adenosyl-L-methionine + phospholipid olefinic fatty acid
Escherichia coli
methylation of unsaturated fatty acid moieties of phospholipids in the phospholipid bilayer
S-adenosyl-L-homocysteine + phospholipid cyclopropane fatty acid
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.1.1.79
Escherichia coli
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.1.1.79
partial
Escherichia coli
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.1.1.79
0.00000113
-
-
Escherichia coli
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.1.1.79
S-adenosyl-L-methionine + cardiolipin
-
485631
Escherichia coli
S-adenosyl-L-homocysteine + ?
-
-
-
?
2.1.1.79
S-adenosyl-L-methionine + phosphatidylethanolamine
-
485631
Escherichia coli
S-adenosyl-L-homocysteine + ?
-
-
-
?
2.1.1.79
S-adenosyl-L-methionine + phosphatidylglycerol
-
485631
Escherichia coli
S-adenosyl-L-homocysteine + ?
-
-
-
?
2.1.1.79
S-adenosyl-L-methionine + phospholipid olefinic fatty acid
reaction is not affected by the order-disorder state of the lipid substrate
485631
Escherichia coli
S-adenosyl-L-homocysteine + phospholipid cyclopropane fatty acid
-
485631
Escherichia coli
?
2.1.1.79
S-adenosyl-L-methionine + phospholipid olefinic fatty acid
methylation of unsaturated fatty acid moieties of phospholipids in the phospholipid bilayer
485631
Escherichia coli
S-adenosyl-L-homocysteine + phospholipid cyclopropane fatty acid
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
2.1.1.79
monomer
1 * 90000, SDS-PAGE
Escherichia coli
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.1.1.79
0.22
-
S-adenosylhomocysteine
-
Escherichia coli
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.1.1.79
5,5'-dithiobis(2-nitrobenzoic acid)
reversed by addition of dithiothreitol
Escherichia coli
2.1.1.79
NEM
-
Escherichia coli
2.1.1.79
p-hydroxymercuribenzoate
-
Escherichia coli
2.1.1.79
S-adenosylhomocysteine
-
Escherichia coli
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.1.1.79
0.22
-
S-adenosylhomocysteine
-
Escherichia coli
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.1.1.79
0.09
-
S-adenosyl-L-methionine
-
Escherichia coli
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.1.1.79
membrane
loosely associated with the inner membrane
Escherichia coli
16020
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.1.1.79
90000
-
equilibrium sedimentation, gel filtration
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.1.1.79
S-adenosyl-L-methionine + phospholipid olefinic fatty acid
Escherichia coli
methylation of unsaturated fatty acid moieties of phospholipids in the phospholipid bilayer
S-adenosyl-L-homocysteine + phospholipid cyclopropane fatty acid
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.1.1.79
partial
Escherichia coli
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.1.1.79
0.00000113
-
-
Escherichia coli
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.1.1.79
S-adenosyl-L-methionine + cardiolipin
-
485631
Escherichia coli
S-adenosyl-L-homocysteine + ?
-
-
-
?
2.1.1.79
S-adenosyl-L-methionine + phosphatidylethanolamine
-
485631
Escherichia coli
S-adenosyl-L-homocysteine + ?
-
-
-
?
2.1.1.79
S-adenosyl-L-methionine + phosphatidylglycerol
-
485631
Escherichia coli
S-adenosyl-L-homocysteine + ?
-
-
-
?
2.1.1.79
S-adenosyl-L-methionine + phospholipid olefinic fatty acid
reaction is not affected by the order-disorder state of the lipid substrate
485631
Escherichia coli
S-adenosyl-L-homocysteine + phospholipid cyclopropane fatty acid
-
485631
Escherichia coli
?
2.1.1.79
S-adenosyl-L-methionine + phospholipid olefinic fatty acid
methylation of unsaturated fatty acid moieties of phospholipids in the phospholipid bilayer
485631
Escherichia coli
S-adenosyl-L-homocysteine + phospholipid cyclopropane fatty acid
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.1.1.79
monomer
1 * 90000, SDS-PAGE
Escherichia coli