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Literature summary extracted from

  • Ichikawa, J.K.; Clarke, S.
    A highly active protein repair enzyme from an extreme thermophile: the L-isoaspartyl methyltransferase from Thermotoga maritima (1998), Arch. Biochem. Biophys., 358, 222-231.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.1.1.77 biotechnology useful in biotechnical applications Thermotoga maritima

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.77 disruption of the pcm gene Caenorhabditis elegans
2.1.1.77 pcm gene PCR amplified, overexpressed in Escherichia coli as a fusion protein Thermotoga maritima

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.77 0.00029
-
 VYP (isoD) HA
-
 Homo sapiens
2.1.1.77 0.00052
-
 KASA (isoD) LAKY
-
 Homo sapiens
2.1.1.77 0.0019
-
 S-adenosyl-L-methionine 37°C Thermotoga maritima
2.1.1.77 0.002
-
 S-adenosyl-L-methionine
-
 Homo sapiens
2.1.1.77 0.0028
-
 KASA (isoD) LAKY 37°C Thermotoga maritima
2.1.1.77 0.0031
-
 S-adenosyl-L-methionine
-
 Caenorhabditis elegans
2.1.1.77 0.0041
-
 VYP (isoD) HA 37°C Thermotoga maritima
2.1.1.77 0.0056
-
 S-adenosyl-L-methionine 85°C Thermotoga maritima
2.1.1.77 0.00912
-
 KASA (isoD) LAKY
-
 Caenorhabditis elegans
2.1.1.77 0.0118
-
 VYP (isoD) HA
-
 Escherichia coli
2.1.1.77 0.0127
-
 KASA (isoD) LAKY
-
 Triticum aestivum
2.1.1.77 0.0194
-
 VYP (isoD) HA
-
 Caenorhabditis elegans
2.1.1.77 0.0217
-
 VYP (isoD) HA
-
 Triticum aestivum
2.1.1.77 0.025
-
 KASA (isoD) LAKY 85°C Thermotoga maritima
2.1.1.77 0.0336
-
 VYP (isoD) HA 85°C Thermotoga maritima
2.1.1.77 0.035
-
 ovalbumin
-
 Homo sapiens
2.1.1.77 0.0506
-
 KASA (isoD) LAKY
-
 Escherichia coli
2.1.1.77 0.326
-
 ovalbumin 37°C Thermotoga maritima
2.1.1.77 0.727
-
 ovalbumin
-
 Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.1.1.77 cytosol
-
 Mus musculus 5829
-
2.1.1.77 cytosol
-
 Escherichia coli 5829
-
2.1.1.77 cytosol
-
 Homo sapiens 5829
-
2.1.1.77 cytosol
-
 Rattus norvegicus 5829
-
2.1.1.77 cytosol
-
 Caenorhabditis elegans 5829
-
2.1.1.77 cytosol
-
 Thermotoga maritima 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.77 34000
-
 1 * 34000, equilibrium centrifugation Thermotoga maritima
2.1.1.77 35000
-
 SDS-PAGE Escherichia coli
2.1.1.77 36400
-
 calculated from translated amino acid sequence Thermotoga maritima

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.77 S-adenosyl-L-methionine + protein L-isoaspartate Mus musculus
-
 S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
-
 ?
2.1.1.77 S-adenosyl-L-methionine + protein L-isoaspartate Escherichia coli
-
 S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
-
 ?
2.1.1.77 S-adenosyl-L-methionine + protein L-isoaspartate Homo sapiens
-
 S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
-
 ?
2.1.1.77 S-adenosyl-L-methionine + protein L-isoaspartate Rattus norvegicus
-
 S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
-
 ?
2.1.1.77 S-adenosyl-L-methionine + protein L-isoaspartate Triticum aestivum
-
 S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
-
 ?
2.1.1.77 S-adenosyl-L-methionine + protein L-isoaspartate Methanothermobacter thermautotrophicus
-
 S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
-
 ?
2.1.1.77 S-adenosyl-L-methionine + protein L-isoaspartate Caenorhabditis elegans
-
 S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
-
 ?
2.1.1.77 S-adenosyl-L-methionine + protein L-isoaspartate Methanocaldococcus jannaschii
-
 S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
-
 ?
2.1.1.77 S-adenosyl-L-methionine + protein L-isoaspartate Archaeoglobus fulgidus
-
 S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
-
 ?
2.1.1.77 S-adenosyl-L-methionine + protein L-isoaspartate Thermotoga maritima highly active protein repair enzyme S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.77 Archaeoglobus fulgidus
-
-
-
2.1.1.77 Caenorhabditis elegans
-
-
-
2.1.1.77 Escherichia coli
-
-
-
2.1.1.77 Homo sapiens
-
 human
-
2.1.1.77 Methanocaldococcus jannaschii
-
-
-
2.1.1.77 Methanothermobacter thermautotrophicus
-
-
-
2.1.1.77 Mus musculus
-
-
-
2.1.1.77 no activity in Aquifex aeolicus
-
-
-
2.1.1.77 no activity in Bacillus subtilis
-
-
-
2.1.1.77 no activity in Borrelia burgdorferi
-
-
-
2.1.1.77 no activity in Haemophilus influenzae
-
-
-
2.1.1.77 no activity in Mycoplasma genitalium
-
-
-
2.1.1.77 no activity in Mycoplasma pneumoniae
-
-
-
2.1.1.77 no activity in Sinorhizobium meliloti
-
-
-
2.1.1.77 no activity in Treponema pallidum
-
-
-
2.1.1.77 Rattus norvegicus
-
-
-
2.1.1.77 Thermotoga maritima
-
-
-
2.1.1.77 Triticum aestivum
-
 wheat
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.77
-
 Escherichia coli
2.1.1.77
-
 Homo sapiens
2.1.1.77
-
 Triticum aestivum
2.1.1.77
-
 Thermotoga maritima

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.77 erythrocyte
-
 Mus musculus
-
2.1.1.77 erythrocyte
-
 Homo sapiens
-
2.1.1.77 erythrocyte
-
 Rattus norvegicus
-
2.1.1.77 germ
-
 Triticum aestivum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.77 0.0022
-
 37°C Thermotoga maritima
2.1.1.77 0.156
-
-
 Thermotoga maritima

Storage Stability

EC Number Storage Stability Organism
2.1.1.77 -80°C, stored in liquid nitrogen, no apparent loss of activity Thermotoga maritima

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.77 S-adenosyl-L-methionine + KASA (isoD) LAKY synthetic methyl acceptor Escherichia coli S-adenosyl-L-homocysteine + ?
-
 ?
2.1.1.77 S-adenosyl-L-methionine + KASA (isoD) LAKY synthetic methyl acceptor Homo sapiens S-adenosyl-L-homocysteine + ?
-
 ?
2.1.1.77 S-adenosyl-L-methionine + KASA (isoD) LAKY synthetic methyl acceptor Triticum aestivum S-adenosyl-L-homocysteine + ?
-
 ?
2.1.1.77 S-adenosyl-L-methionine + KASA (isoD) LAKY synthetic methyl acceptor Caenorhabditis elegans S-adenosyl-L-homocysteine + ?
-
 ?
2.1.1.77 S-adenosyl-L-methionine + KASA (isoD) LAKY synthetic methyl acceptor Thermotoga maritima S-adenosyl-L-homocysteine + ?
-
 ?
2.1.1.77 S-adenosyl-L-methionine + ovalbumin
-
 Escherichia coli S-adenosyl-L-homocysteine + ?
-
 ?
2.1.1.77 S-adenosyl-L-methionine + ovalbumin
-
 Homo sapiens S-adenosyl-L-homocysteine + ?
-
 ?
2.1.1.77 S-adenosyl-L-methionine + ovalbumin
-
 Rattus norvegicus S-adenosyl-L-homocysteine + ?
-
 ?
2.1.1.77 S-adenosyl-L-methionine + ovalbumin
-
 Triticum aestivum S-adenosyl-L-homocysteine + ?
-
 ?
2.1.1.77 S-adenosyl-L-methionine + ovalbumin
-
 Caenorhabditis elegans S-adenosyl-L-homocysteine + ?
-
 ?
2.1.1.77 S-adenosyl-L-methionine + ovalbumin
-
 Thermotoga maritima S-adenosyl-L-homocysteine + ?
-
 ?
2.1.1.77 S-adenosyl-L-methionine + protein L-isoaspartate
-
 Mus musculus S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
-
 ?
2.1.1.77 S-adenosyl-L-methionine + protein L-isoaspartate
-
 Escherichia coli S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
-
 ?
2.1.1.77 S-adenosyl-L-methionine + protein L-isoaspartate
-
 Homo sapiens S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
-
 ?
2.1.1.77 S-adenosyl-L-methionine + protein L-isoaspartate
-
 Rattus norvegicus S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
-
 ?
2.1.1.77 S-adenosyl-L-methionine + protein L-isoaspartate
-
 Triticum aestivum S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
-
 ?
2.1.1.77 S-adenosyl-L-methionine + protein L-isoaspartate
-
 Methanothermobacter thermautotrophicus S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
-
 ?
2.1.1.77 S-adenosyl-L-methionine + protein L-isoaspartate
-
 Caenorhabditis elegans S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
-
 ?
2.1.1.77 S-adenosyl-L-methionine + protein L-isoaspartate
-
 Methanocaldococcus jannaschii S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
-
 ?
2.1.1.77 S-adenosyl-L-methionine + protein L-isoaspartate
-
 Archaeoglobus fulgidus S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
-
 ?
2.1.1.77 S-adenosyl-L-methionine + protein L-isoaspartate
-
 Thermotoga maritima S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
-
 ?
2.1.1.77 S-adenosyl-L-methionine + protein L-isoaspartate highly active protein repair enzyme Thermotoga maritima S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
-
 ?
2.1.1.77 S-adenosyl-L-methionine + VYP (isoD) HA
-
 Escherichia coli S-adenosyl-L-homocysteine + ?
-
 ?
2.1.1.77 S-adenosyl-L-methionine + VYP (isoD) HA
-
 Homo sapiens S-adenosyl-L-homocysteine + ?
-
 ?
2.1.1.77 S-adenosyl-L-methionine + VYP (isoD) HA
-
 Triticum aestivum S-adenosyl-L-homocysteine + ?
-
 ?
2.1.1.77 S-adenosyl-L-methionine + VYP (isoD) HA
-
 Caenorhabditis elegans S-adenosyl-L-homocysteine + ?
-
 ?
2.1.1.77 S-adenosyl-L-methionine + VYP (isoD) HA
-
 Thermotoga maritima S-adenosyl-L-homocysteine + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.77 monomer
-
 Escherichia coli
2.1.1.77 monomer
-
 Homo sapiens
2.1.1.77 monomer 1 * 34000, equilibrium centrifugation Thermotoga maritima

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.77 85
-
-
 Thermotoga maritima

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.1.1.77 37 93
-
 Thermotoga maritima

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.1.1.77 100
-
 extremely heat stable, no loss of activity after 60 min Thermotoga maritima

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.77 5.5
-
-
 Escherichia coli
2.1.1.77 5.6
-
-
 Thermotoga maritima
2.1.1.77 6 7
-
 Rattus norvegicus
2.1.1.77 7.5
-
-
 Rattus norvegicus
2.1.1.77 7.5
-
-
 Triticum aestivum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.1.1.77 5 8.5
-
 Thermotoga maritima