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Literature summary extracted from

  • Eckardt, N.A.
    Probing the mysteries of lignin biosynthesis: The crystal structure of caffeic acid/5-hydroxyferulic acid 3/5-O-methyltransferase provides new insights (2002), Plant Cell, 14, 1185-1189.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.1.1.68
-
 Medicago sativa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.68 0.005
-
 5-hydroxyconiferaldehyde
-
 Medicago sativa
2.1.1.68 0.01
-
 5-hydroxyferulic acid
-
 Medicago sativa
2.1.1.68 0.043
-
 caffeic acid
-
 Medicago sativa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.68 S-adenosyl-L-methionine + 3,4-dihydroxy-trans-cinnamate Medicago sativa i.e. caffeic acid, involved in the biosynthesis of lignin S-adenosyl-L-homocysteine + 3-methoxy-4-hydroxy-trans-cinnamate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.68 Medicago sativa
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.68 additional information bifunctional enzyme, methylation of 3-hydroxy and 5-hydroxy positions on the aromatic ring Medicago sativa ?
-
 ?
2.1.1.68 S-adenosyl-L-methionine + 3,4-dihydroxy-trans-cinnamate
-
 Medicago sativa S-adenosyl-L-homocysteine + 3-methoxy-4-hydroxy-trans-cinnamate i.e. ferulate ?
2.1.1.68 S-adenosyl-L-methionine + 3,4-dihydroxy-trans-cinnamate i.e. caffeic acid, involved in the biosynthesis of lignin Medicago sativa S-adenosyl-L-homocysteine + 3-methoxy-4-hydroxy-trans-cinnamate
-
 ?
2.1.1.68 S-adenosyl-L-methionine + 5-hydroxyferulic acid
-
 Medicago sativa S-adenosyl-L-homocysteine + sinapic acid
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.68 COMT
-
 Medicago sativa