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Literature summary extracted from

  • Bhattacharyya, D.; Hazra, T.K.; Behnke, W.D.; Chong, P.L.; Kurosky, A.; Lee, J.C.; Mitra, S.
    Reversible folding of Ada protein (O6-methylguanine-DNA methyltransferase) of Escherichia coli (1998), Biochemistry, 37, 1722-1730.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.1.1.63 enzyme is denatured by 8 M urea at pH 7.5 for 30 s or by 6 M guanidine hydrochloride at pH 7.5 for 16-24 h Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.63 39356
-
 x * 39356, inactive enzyme, electrospray mass spectrometry Escherichia coli
2.1.1.63 39377
-
 x * 39377, active enzyme, electrospray mass spectrometry Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.63 DNA containing 6-O-methylguanine + [protein]-L-cysteine Escherichia coli the enzyme functions in DNA repair by direct dealkylation of mutagenic 6-O-alkylguanine. The protein methylated at Cys69 becomes a transcriptional activator of the genes in the ada regulon, including its own DNA lacking 6-O-methylguanine + [protein]-S-methyl-L-cysteine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.63 Escherichia coli
-
-
-

Renatured (Commentary)

EC Number Renatured (Comment) Organism
2.1.1.63 renaturation by dilution with buffer from 8 M urea or 6 M guanidine HCl instantly restores the secondary structure of the Ada protein to a state that is almost indistinguishable from the native state. Kinetics of renaturation is fast, at 0°C the restoration is quantitative in less than 30 s. The heat-coagulated protein can be restored to full activity by cycling it through treatment with 8 M urea or 6 M guanidine Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.63 DNA containing 6-O-methylguanine + [protein]-L-cysteine the enzyme functions in DNA repair by direct dealkylation of mutagenic 6-O-alkylguanine. The protein methylated at Cys69 becomes a transcriptional activator of the genes in the ada regulon, including its own Escherichia coli DNA lacking 6-O-methylguanine + [protein]-S-methyl-L-cysteine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.63 ? x * 39356, inactive enzyme, electrospray mass spectrometry Escherichia coli
2.1.1.63 ? x * 39377, active enzyme, electrospray mass spectrometry Escherichia coli

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.1.1.63 additional information
-
 temperature-dependent inactivation of the enzyme is inhibited by DNA Escherichia coli
2.1.1.63 37
-
 spontaneously loses about 30% of its secondary structure in less than 30 min concomitant with a complete loss of activity Escherichia coli
2.1.1.63 70
-
 protein immediately coagulates Escherichia coli