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Literature summary extracted from

  • Chan, C.L.; Wu, Z.; Ciardelli, T.; Eastman, A.; Bresnick, E.
    Kinetic and DNA-binding properties of recombinant human O6-methylguanine-DNA methyltransferase (1993), Arch. Biochem. Biophys., 300, 193-200.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.63 cDNA is cloned into a pET-11a plasmid and expressed in Escherichia coli Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.63 Homo sapiens
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Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.63 recombinant enzyme Homo sapiens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.63 additional information
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 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.63 DNA containing 6-O-methylguanine + [protein]-L-cysteine the binding to DNA is the rate determining step in the repair process. Approximately eight base pairs of the DNA substrate are covered by the human enzyme. Binding affinity to methylated DNA is two times higher than that to unmodified DNA. The interaction with DNA induces a conformational change in the enzyme Homo sapiens DNA lacking 6-O-methylguanine + [protein]-S-methyl-L-cysteine
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