Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Leclere, M.M.; Nishioka, M.; Yuasa, T.; Fujiwara, S.; Takagi, M.; Imanaka, T.
    The O6-methylguanine-DNA methyltransferase from the hyperthermophilic archaeon Pyrococcus sp. KOD1: a thermostable repair enzyme (1998), Mol. Gen. Genet., 258, 69-77.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.63 the gene is placed under the control of the lac promoter and overexpressed in the methyltransferase-deficient Escherichia coli strain KT233 Pyrococcus sp.

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.63 Pyrococcus sp.
-
 KOD1
-
2.1.1.63 Pyrococcus sp. KOD1
-
 KOD1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.63 recombinant enzyme Pyrococcus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.63 DNA containing 6-O-methylguanine + [protein]-L-cysteine
-
 Pyrococcus sp. DNA lacking 6-O-methylguanine + [protein]-S-methyl-L-cysteine
-
 ?
2.1.1.63 DNA containing 6-O-methylguanine + [protein]-L-cysteine
-
 Pyrococcus sp. KOD1 DNA lacking 6-O-methylguanine + [protein]-S-methyl-L-cysteine
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.1.1.63 75
-
 60 min, 25% loss of activity Pyrococcus sp.
2.1.1.63 90
-
 stable for at least 30 min. 66% loss of activity after 60 min Pyrococcus sp.