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Literature summary extracted from

  • Durban, E.; Nochumson, S.; Kim, S.; Ki Paik, W.
    Cytochrome c-specific protein-lysine methyltransferase from Neurospora crassa. Purification, characterization, and substrate requirements (1978), J. Biol. Chem., 253, 1427-1435.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.59 S-adenosyl-L-homocysteine competitive versus S-adenosyl-L-methionine Neurospora crassa
2.1.1.59 S-adenosyl-L-methionine
-
 Neurospora crassa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.59 0.007
-
 CNBr peptide residue 1-80 of horse heart cytochrome c Neurospora crassa
2.1.1.59 0.03
-
 [cytochrome c]-L-lysine of horse heart, heme-free Neurospora crassa
2.1.1.59 0.04
-
 CNBr peptide residue 1-65 or residue 66-104 of horse heart cytochrome c Neurospora crassa
2.1.1.59 0.2
-
 [cytochrome c]-L-lysine of horse heart, ethanol-denatured Neurospora crassa
2.1.1.59 0.32
-
 [cytochrome c]-L-lysine of horse heart, native Neurospora crassa
2.1.1.59 1.7
-
 [cytochrome c]-L-lysine
-
 Neurospora crassa
2.1.1.59 19
-
 S-adenosyl-L-methionine
-
 Neurospora crassa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.1.1.59 cytosol
-
 Neurospora crassa 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.59 120000
-
 gel filtration Neurospora crassa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.59 S-adenosyl-L-methionine + cytochrome c L-lysine Neurospora crassa
-
 S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.59 Neurospora crassa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.59
-
 Neurospora crassa

Reaction

EC Number Reaction Comment Organism Reaction ID
2.1.1.59 S-adenosyl-L-methionine + [cytochrome c]-L-lysine = S-adenosyl-L-homocysteine + [cytochrome c]-N6-methyl-L-lysine specific methylation site, 1 lysine in cytochrome c, comparison Neurospora crassa
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.59 0.0295
-
 purified enzyme Neurospora crassa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.59 S-adenosyl-L-methionine + CNBr peptide
-
 Neurospora crassa ?
-
 ?
2.1.1.59 S-adenosyl-L-methionine + cytochrome c L-lysine
-
 Neurospora crassa S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine
-
 ?
2.1.1.59 S-adenosyl-L-methionine + cytochrome c L-lysine horse heart cytochrome c-72 Neurospora crassa S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine a single lysine residue at position 72 of horse heart cytochrome c is methylated ?
2.1.1.59 S-adenosyl-L-methionine + cytochrome c L-lysine horse heart cytochrome c-72 Neurospora crassa S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine mixture of epsilon-N-monomethyllysine, epsilon-N-dimethyllysine and epsilon-N-trimethyllysine in the ratio 1:3:4 ?
2.1.1.59 S-adenosyl-L-methionine + cytochrome c L-lysine important features in determining methyl-accepting activity: 1. reactive site such as-X-Lys-Lys-Y-, 2. length of polypeptide chain, 3. tertiary structure Neurospora crassa S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine a single lysine residue at position 72 of horse heart cytochrome c is methylated ?
2.1.1.59 S-adenosyl-L-methionine + cytochrome c L-lysine important features in determining methyl-accepting activity: 1. reactive site such as-X-Lys-Lys-Y-, 2. length of polypeptide chain, 3. tertiary structure Neurospora crassa S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine mixture of epsilon-N-monomethyllysine, epsilon-N-dimethyllysine and epsilon-N-trimethyllysine in the ratio 1:3:4 ?
2.1.1.59 S-adenosyl-L-methionine + cytochrome c L-lysine does not randomly methylate cytochrome c, but shows absolute specificity for some amino acid sequences Neurospora crassa S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine a single lysine residue at position 72 of horse heart cytochrome c is methylated ?
2.1.1.59 S-adenosyl-L-methionine + cytochrome c L-lysine does not randomly methylate cytochrome c, but shows absolute specificity for some amino acid sequences Neurospora crassa S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine mixture of epsilon-N-monomethyllysine, epsilon-N-dimethyllysine and epsilon-N-trimethyllysine in the ratio 1:3:4 ?
2.1.1.59 S-adenosyl-L-methionine + cytochrome c L-lysine also CNBr peptides of horse heart cytochrome c can serve as substrates, overview Neurospora crassa S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine a single lysine residue at position 72 of horse heart cytochrome c is methylated ?
2.1.1.59 S-adenosyl-L-methionine + cytochrome c L-lysine also CNBr peptides of horse heart cytochrome c can serve as substrates, overview Neurospora crassa S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine mixture of epsilon-N-monomethyllysine, epsilon-N-dimethyllysine and epsilon-N-trimethyllysine in the ratio 1:3:4 ?
2.1.1.59 S-adenosyl-L-methionine + cytochrome c L-lysine overview: substrate activity of various cytochromes c Neurospora crassa S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine a single lysine residue at position 72 of horse heart cytochrome c is methylated ?
2.1.1.59 S-adenosyl-L-methionine + cytochrome c L-lysine overview: substrate activity of various cytochromes c Neurospora crassa S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine mixture of epsilon-N-monomethyllysine, epsilon-N-dimethyllysine and epsilon-N-trimethyllysine in the ratio 1:3:4 ?
2.1.1.59 S-adenosyl-L-methionine + [cytochrome c]-L-lysine
-
 Neurospora crassa S-adenosyl-L-homocysteine + [cytochrome c]-N6-methyl-L-lysine
-
 r

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.59 37
-
 assay at Neurospora crassa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.59 9
-
-
 Neurospora crassa

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.1.1.59 0.002
-
 S-adenosyl-L-methionine
-
 Neurospora crassa