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Literature summary extracted from

  • Nochumson, S.; Durban, E.; Kim, S.; Paik, W.K.
    Cytochrome c-specific protein methylase III from Neurospora crassa (1977), Biochem. J., 165, 11-18.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.59 0.0095
-
 S-adenosyl-L-methionine
-
 Neurospora crassa
2.1.1.59 0.12
-
 [cytochrome c]-L-lysine
-
 Neurospora crassa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.1.1.59 cytosol 74.3% of the enzyme activity Neurospora crassa 5829
-
2.1.1.59 lipid bilayer 13.6% of the enzyme activity Neurospora crassa
-
-
2.1.1.59 membrane
-
 Neurospora crassa 16020
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.59 S-adenosyl-L-methionine + cytochrome c L-lysine Neurospora crassa
-
 S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.59 Neurospora crassa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.59 partially Neurospora crassa

Reaction

EC Number Reaction Comment Organism Reaction ID
2.1.1.59 S-adenosyl-L-methionine + [cytochrome c]-L-lysine = S-adenosyl-L-homocysteine + [cytochrome c]-N6-methyl-L-lysine specific methylation site, 1 lysine in cytochrome c, comparison Neurospora crassa
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.59 mycelium
-
 Neurospora crassa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.59 0.000289
-
 partially purified enzyme Neurospora crassa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.59 S-adenosyl-L-methionine + cytochrome c L-lysine
-
 Neurospora crassa S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine
-
 ?
2.1.1.59 S-adenosyl-L-methionine + cytochrome c L-lysine horse heart cytochrome c-72 Neurospora crassa S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine mixture of epsilon-N-monomethyllysine, epsilon-N-dimethyllysine and epsilon-N-trimethyllysine in the ratio 1:3:4 ?
2.1.1.59 S-adenosyl-L-methionine + cytochrome c L-lysine does not randomly methylate cytochrome c, but shows absolute specificity for some amino acid sequences Neurospora crassa S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine mixture of epsilon-N-monomethyllysine, epsilon-N-dimethyllysine and epsilon-N-trimethyllysine in the ratio 1:3:4 ?
2.1.1.59 S-adenosyl-L-methionine + myoglobin L-lysine 10% activity compared to cytochrome c Neurospora crassa S-adenosyl-L-homocysteine + myoglobin N6-methyl-L-lysine
-
 ?
2.1.1.59 S-adenosyl-L-methionine + [cytochrome c]-L-lysine
-
 Neurospora crassa S-adenosyl-L-homocysteine + [cytochrome c]-N6-methyl-L-lysine
-
 r

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.59 9
-
-
 Neurospora crassa