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Literature summary extracted from

  • Ramadevi, N.; Burroughs, N.J.; Mertens, P.P.C.; Jones, I.M.
    Capping and methylation of mRNA by purified recombinant VP4 protein of bluetongue virus (1998), Proc. Natl. Acad. Sci. USA, 95, 13537-13542.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.56 expression of VP4 protein in Spodoptera frugiperda cells bluetongue virus
2.1.1.57 expression of VP4 protein in Spodoptera frugiperda cells bluetongue virus

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.56 additional information mapping of lipid-binding site, membrane binding activity of wild-type and diverse constructed mutants bluetongue virus

General Stability

EC Number General Stability Organism
2.1.1.56 cardiolipin, phosphatidylglycerol, and phosphatidylserine bluetongue virus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.56 3-[(3-cholamidopropyl) dimethylammonio]-1-propanesulfonate i.e. CHAPS, strong inhibition bluetongue virus
2.1.1.56 deoxycholate inactivation bluetongue virus
2.1.1.56 Digitonin strong inhibition bluetongue virus
2.1.1.56 additional information detergents prevent the association of S-adenosyl-L-methionine with the enzyme bluetongue virus
2.1.1.56 N,N-bis-(3-D-gluconamidopropyl)-deoxycholamide strong inhibition bluetongue virus
2.1.1.56 n-octyl-beta-D-gluconpyranoside i.e. octylglucoside, strong inhibition bluetongue virus
2.1.1.56 N-tetradecyl-N,N-dimethyl-3-ammonio-1-propane sulfonate i.e. zwittergent 3-14, strong inhibition bluetongue virus
2.1.1.56 Thesit strong inhibition bluetongue virus
2.1.1.56 Triton X-100 inactivation, reversible by addition of lipids: cardiolipin, phosphatidylglycerol, and especially phosphatidylserine bluetongue virus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.1.1.56 membrane lipid-binding site, membrane binding activity of wild-type and diverse mutants bluetongue virus 16020
-
2.1.1.56 viral nucleocapsid
-
 bluetongue virus 19013
-
2.1.1.57 viral nucleocapsid
-
 bluetongue virus 19013
-

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.56 bluetongue virus
-
 enzyme activity belongs to VP4 protein
-
2.1.1.57 bluetongue virus
-
 enzyme activity belongs to VP4 protein
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.56 recombinant from Spodoptera frugiperda cells bluetongue virus
2.1.1.57 recombinant from Spodoptera frugiperda cells bluetongue virus

Reaction

EC Number Reaction Comment Organism Reaction ID
2.1.1.56 S-adenosyl-L-methionine + a 5'-(5'-triphosphoguanosine)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N7-methyl 5'-triphosphoguanosine)-[mRNA] RNA triphosphatase, RNA guanylyltransferase, and RNA (guanine-N7-)methyltransferase form the mRNA capping enzyme complex consiting of a small and a large subunit bluetongue virus
a
2.1.1.56 S-adenosyl-L-methionine + a 5'-(5'-triphosphoguanosine)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N7-methyl 5'-triphosphoguanosine)-[mRNA] lipid-binding site, membrane binding activity of wild-type and diverse mutants bluetongue virus
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.56 S-adenosyl-L-methionine + G(5')pppN N: G bluetongue virus S-adenosyl-L-homocysteine + m7G(5')pppG
-
 ?
2.1.1.56 S-adenosyl-L-methionine + G(5')pppN unmethylated cap analogue, serves as in vitro substrate bluetongue virus S-adenosyl-L-homocysteine + m7G(5')pppG
-
 ?
2.1.1.56 S-adenosyl-L-methionine + G(5')pppR-RNA
-
 bluetongue virus S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
 ?
2.1.1.57 S-adenosyl-L-methionine + m7G(5')pppR-RNA
-
 bluetongue virus S-adenosyl-L-homocysteine + m7G(5')pppRm-RNA
-
 ?
2.1.1.57 S-adenosyl-L-methionine + m7GpppG
-
 bluetongue virus S-adenosyl-L-homocysteine + m7GpppGm
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.56 30
-
 assay at bluetongue virus
2.1.1.57 30
-
 assay at bluetongue virus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.56 7.5
-
 assay at bluetongue virus
2.1.1.57 7.5
-
 assay at bluetongue virus